Aged Garlic Extract Inhibits Angiogenesis and Proliferation of Colorectal Carcinoma Cells

Abstract

Because colorectal cancer is likely to develop in many people at some point during their lives, prevention has become a high priority. Diet and nutrition play an important role during the multistep colon carcinogenic process. Garlic has been traditionally used as a spice and is well known for its medicinal properties; several studies have indicated its pharmacologic functions, including its anticarcinogenic properties. However, the mechanisms by which garlic can prevent colorectal cancer remain to be elucidated. This study investigated the effect of aged garlic extract (AGE) on the growth of colorectal cancer cells and their angiogenesis, which are important microenvironmental factors in carcinogenesis. AGE suppressed the proliferation of 3 different colorectal cancer cell lines-HT29, SW480, and SW620-in the same way, but its effects on the invasive activities of these 3 cell lines were different. the invasive activities of SW480 and SW620 cells were inhibited by AGE, whereas AGE had no effect on the invasive activity of Ht29 cells. The action of AGE appears to be dependent on the type of cancer cell. On the other hand, AGE enhanced the adhesion of endothelial cells to collagen and fibronectin and suppressed cell motility and invasion. AGE also inhibited the proliferation and tube formation of endothelial cells potently. These results suggest that AGE could prevent tumor formation by inhibiting angiogenesis through the suppression of endothelial cell motility, proliferation, and tube formation. AGE would be a good chemopreventive agent for colorectal cancer because of its antiproliferative action on colorectal carcinoma cells and inhibitory activity on angiogenesis.

Concordant VS. Discordant Xeno Cardiac Transplantation; The past, the present and future Trends

“…Garlic is traditionally used as a spice and is known for its medicinal properties and varied pharmacologic functions including antimicrobial, antithrombotic, hypolipidimic and anticarcinogenic effects (28)(29)(30)(31)(32)(33)(34)(35) . Epidemiologic studies have also provided evidence that increased intake of garlic is associated with decreased cancer risk (36) .…”

Ultrapathological evaluation of the anticancer effect of blackseed (Nigella sativa) and garlic (Allium sativum) in mice

“…These findings were similar to those obtained by Khan and Sultana,31 who recorded that Nigella sativa is a potent chemopreventive agent and suppresses Fe-NTA-induced oxidative stress, hyperproliferative response and renal carcinogenesis in Wistar rats. Garlic and its allyl sulfur may exert their protective effects on carcinogenesis in animal and in vitro by several mechanisms, including inhibition of carcinogen-induced DNA adduct formation, 32 blockage of cell growth, blockage of cell proliferation, and blockage of angiogenesis, 33 induction of differentiation and/or apoptosis, 5,34 enhancement of carcinogen-detoxifying enzymes, 35 and/or suppression of carcinogenactivating enzymes, 36 inhibition of cyclooxygenase-2 expression, 37 scavenging carcinogeninduced free radicals, 38,39 and inhibition of lipid peroxidation. 39,40 Another possible mechanism of cancer prevention and suppression of cancer progression is immune enhancement by garlic, 9 and Nigella sativa, 21 and these agreed with our hematologic and histopathological findings.…”

Targeting Colon Drug Delivery by Natural Products

“…Aged garlic extract (AGE) has manifold biological activities including immune-modulated and anti-oxidative effects. It is used as a major component of nonprescription tonics and cold-prevention medicines or dietary supplements [30,31].…”