Aged garlic extract modulates glutathione redox cycle and superoxide dismutase activity in vascular endothelial cells

Geng, Zhi-Hui; Lau, Benjamin H.S.

doi:10.1002/(sici)1099-1573(199702)11:1<54::aid-ptr950>3.0.co;2-y

Cited by 56 publications

(5 citation statements)

References 9 publications

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“…It inhibited the oxidation of isolated human LDL-C by scavenging peroxides and inhibiting the formation of lipid peroxides [45]. AGE (aged garlic extract) acted as an antioxidant by scavenging the reactive oxygen species [121] and increasing the activity of antioxidant enzymes in vascular endothelial cells [122]. AGE also prevented the reduction of intracellular glutathione (GSH) concentration when incubating endothelial cells with oxidized LDL-C [123].…”

Section: Animal Studymentioning

confidence: 99%

The Influence of Plants from the Alliaceae Family on Morphological Parameters of the Intestine in Atherogenic Rats

Najman

Leontowicz

2021

Nutrients

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Bulbs from the Alliaceae family have been well-known and valued spices for thousands of years, not only for their unique flavor and aroma features, but also for their high nutritional and health-promoting values. Long-term or excessive consumption of these vegetables, especially raw garlic, can have side effects in the body (including in the digestive tract), causing a number of pathological changes in the intestinal wall; these changes lead, in turn, to its damage, dysfunction, and disorder development. Therefore, the aim of this study was to investigate the effect of the addition of freeze-dried vegetables from the Alliaceae family, i.e., garlic (Allium sativum L.), white onion, and red onion (Allium cepa L.) on the morphometric parameters (intestinal villi length, crypt depth, thickness of tunica mucosa, and the thickness of tunica muscle) of the jejunum of rats fed a semi-synthetic atherogenic diet (1% dietary cholesterol). In freeze-dried vegetables administered to rats, the contents of selected bioactive ingredients and their antioxidant potentials were determined. The effect of the onion vegetable supplements on growth parameters, serum lipid profile, plasma antioxidant potential, and the intestinal morphological parameters of rats loaded with cholesterol was determined. In an animal experiment, 30 male Wistar rats were divided into 5 diet groups, diet consumption and FER were studied. Supplementation of the atherogenic diet with vegetables improved the blood plasma lipid profiles and atherogenic indices, in a manner that was dependent on the type of supplementation used, with the best hypolipidemic and anti-atherosclerotic effects found in garlic use. The atherogenic diet, as well as the supplementation of this diet with the tested vegetables from the Alliaceae family, influenced the histological changes in the epithelium of the jejunum of rats. The damage to the intestinal mucosa was the greatest in animals fed an atherogenic diet supplemented with garlic. Bearing in mind that the desired beneficial therapeutic or prophylactic effects of onion vegetables (in particular garlic) in the course of various metabolic ailments (including atherosclerosis) are achieved during long-term supplementation, it is important to remember their possible cytotoxic effects (e.g., on the digestive tract) in order to achieve real benefits related to the supplementation with vegetables from the Alliaceae family.

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Section: Animal Studymentioning

confidence: 99%

The Influence of Plants from the Alliaceae Family on Morphological Parameters of the Intestine in Atherogenic Rats

Najman

Leontowicz

2021

Nutrients

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“…The intrinsic antioxidant activity of garlic (Rietz et al 1993), garlic extracts (Prasad et al 1996) and some garlic constituents (Rabinkov et al 1998) has been widely documented in-vivo (Augusti & Sheela 1996) and in-vitro (Prasad et al 1996;Rabinkov et al 1998). Garlic extracts increase superoxide dismutase (SOD) (Geng & Lau 1997), glutathione peroxidase (GPx) (Wei & Lau 1998) and catalase (CAT) activity (Wei & Lau 1998) in vascular cell cultures, while a garlic compound, S-allyl-cysteine sulfoxide (alliin), prevents the decreases in hepatic SOD and CAT activity observed in diabetic rats (Augusti & Sheela 1996).…”

Section: Introductionmentioning

confidence: 99%

Aqueous garlic extract alleviates ischaemia-reperfusion-induced oxidative hepatic injury in rats

Şener

Şehirli

İpçi

et al. 2005

Journal of Pharmacy and Pharmacology

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This study was designed to examine the effects of aqueous garlic extract (AGE) on hepatic ischaemia-reperfusion (I/R) injury in rats. For this purpose, Wistar albino rats were subjected to 45 min of hepatic ischaemia, followed by a 60-min reperfusion period. AGE (1 mL kg(-1), i. p., corresponding to 500 mg kg(-1)) or saline was administered twice, 15 min before ischaemia and immediately before the reperfusion period. Serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels were determined to assess liver functions. Liver tissues were taken for the determination of malondialdehyde (MDA) levels, an end product of lipid peroxidation; glutathione (GSH) levels, a key antioxidant; and myeloperoxidase (MPO) activity, as an indirect index of neutrophil infiltration. Hepatic collagen content, as a fibrosis marker, was also determined. Plasma ALT and AST activities were elevated in the I/R group as compared with the control group, while these increases were significantly decreased by AGE treatment. Hepatic GSH levels, significantly depressed by I/R, were elevated back to control levels in the AGE-treated I/R group. Increases in tissue MDA levels and MPO activity due to I/R injury were reduced back to control levels by AGE treatment. Similarly, increased hepatic collagen content in the I/R group was reduced to the control level with AGE treatment. Since AGE administration alleviated the I/R-induced injury of the liver and improved the hepatic structure and function, it seems likely that AGE, with its antioxidant and oxidant-scavenging properties, may be of potential therapeutic value in protecting the liver against oxidative injury due to ischaemia-reperfusion.

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“…The intrinsic antioxidant activity of garlic (Rietz et al 1993), garlic extracts (Numagami et al 1996;Prasad et al 1996) and some garlic constituents (Ide et al 1996;Rabinkov et al 1998) has been widely documented in-vivo (Augusti & Sheela 1996;Iqbal & Athar 1998) and in-vitro (Prasad et al 1996;Rabinkov et al 1998). Garlic extracts increase superoxide dismutase (Geng & Lau 1997), glutathione peroxidase (Wei & Lau 1998), and catalase activity (Wei & Lau 1998) in vascular cells in culture, and S-allyl-cysteine sulfoxide (alliin), a garlic compound, prevents the decrease in hepatic superoxide dismutase and catalase activity observed in diabetic rats (Augusti & Sheela 1996). S¸ener et al (2003a, b) previously demonstrated that aqueous garlic extract (AGE) protects against oxidative tissue damage induced by thermal trauma or ischaemia/reperfusion in rats.…”

Section: Introductionmentioning

confidence: 99%

Protective effect of aqueous garlic extract against naphthalene-induced oxidative stress in mice

Omurtag

Güranlioǧlu

Şehirli

et al. 2005

Journal of Pharmacy and Pharmacology

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The aim of this study was to investigate the possible protective effects of aqueous garlic extract (AGE) against naphthalene-induced oxidative changes in liver, kidney, lung and brain of mice. Balb/c mice (25-30 g) of either sex were divided into five groups each comprising 10 animals. Mice received for 30 days: 0.9% NaCl, i.p. (control); corn oil, i.p; AGE in a dose of 125 mg kg-1, i.p.; naphthalene in a dose of 100 mg kg-1, i.p. (dissolved in corn oil); and AGE (in a dose of 125 mg kg-1, i.p.) plus naphthalene (in a dose of 100 mg kg-1, i.p.). After decapitation, liver, kidney, lung and brain tissues were excised. Malondialdehyde (MDA) and glutathione (GSH) levels and myeloperoxidase activity (MPO) were determined in the tissues, while oxidant-induced tissue fibrosis was determined by collagen content. Tissues were also examined microscopically. Serum aspartate aminotransferase, alanine aminotransferase levels and blood urea nitrogen and creatinine concentrations were measured for the evaluation of hepatic and renal function, respectively. MDA and GSH levels were also assayed in serum samples. In the naphthalene-treated group, GSH levels decreased significantly, while MDA levels, MPO activity and collagen content increased in the tissues (P<0.01-0.001), suggesting oxidative organ damage, which was also verified histologically. In the AGE-treated naphthalene group, all of these oxidant responses were reversed significantly (P<0.05-0.01). Hepatic and renal function test parameters, which increased significantly (P<0.001) following naphthalene administration, decreased (P<0.05-0.001) after AGE treatment. The results demonstrate the role of oxidative mechanisms in naphthalene-induced tissue damage. The antioxidant properties of AGE ameliorated oxidative organ injury due to naphthalene toxicity.

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Aged garlic extract modulates glutathione redox cycle and superoxide dismutase activity in vascular endothelial cells

Cited by 56 publications

References 9 publications

The Influence of Plants from the Alliaceae Family on Morphological Parameters of the Intestine in Atherogenic Rats

The Influence of Plants from the Alliaceae Family on Morphological Parameters of the Intestine in Atherogenic Rats

Aqueous garlic extract alleviates ischaemia-reperfusion-induced oxidative hepatic injury in rats

Protective effect of aqueous garlic extract against naphthalene-induced oxidative stress in mice

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