Aged keratinocyte phenotyping: Morphology, biochemical markers and effects of Dead Sea minerals

Soroka, Yoram; Ma’or, Ze’evi; Leshem, Yael; Verochovsky, Lilian; Neuman, Rami; Brégégère, François; Milner, Yoram

doi:10.1016/j.exger.2008.08.003

Cited by 54 publications

(52 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An additional anti-aging cream, Dermud™ (Ahava Co. Israel), composed of Dead Sea minerals and plant extracts was also used in these experiments. This cream has been tested in skin explants in vitro using molecular markers as a readout for its anti-apoptotic effect (Portugal-Cohen et al 2009;Soroka et al 2008). The creams were spread at about 2 mg/cm 2 on saran wrap that was placed between the UV source and the Petri dish with the cells.…”

Section: Methodsmentioning

confidence: 99%

“…In these experiments, the explants were pretreated for 48 h prior to UV irradiation with "Mineral Skin Osmoter," a solution rich in 28 elements, and were irradiated in the absence of the product. The authors found that the pretreatment stimulated cell proliferation and activity of mitochondria, decreased the expression of markers associated with aging, and limited UVB-induced apoptosis (Soroka et al 2008). In subsequent experiments, human skin organ cultures pretreated with Dermud™ (Ahava Co.), composed of Dead Sea mud, 1% Osmoter solution, zinc oxide, aloe vera extract, provitamin B5, vitamin E, and additional plant extracts, were protected against oxidizing radicals and showed reduced photodamage and photoaging after UVB irradiation (Portugal-Cohen et al 2009).…”

Section: Protection Of Cells Irradiated With Uvb By Sunscreen Compoundsmentioning

confidence: 98%

“…Cosmetic products that include mineral-rich mud from the Dead Sea were reported to display anti-aging properties in epidermal keratinocyte cultures and skin explants in vitro (Soroka et al 2008). In these experiments, the explants were pretreated for 48 h prior to UV irradiation with "Mineral Skin Osmoter," a solution rich in 28 elements, and were irradiated in the absence of the product.…”

Section: Protection Of Cells Irradiated With Uvb By Sunscreen Compoundsmentioning

confidence: 99%

“…To determine whether this yeast bioluminescence assay could be used to determine the efficacy of sunscreen creams of different SPF, two different Dr Fischer sunscreens of SPF15 and SPF100 and Dermud™ (Ahava Co.) cream reported to antagonize the biological effects of UVB irradiation on skin (Portugal-Cohen et al 2009;Soroka et al 2008) were spread on saran wrap between the UV source and the Petri dish containing the engineered yeast cells. Body cream (Indola Co.) was used as a control.…”

Section: Protection Of Cells Irradiated With Uvb By Sunscreen Compoundsmentioning

confidence: 99%

See 3 more Smart Citations

UV and arsenate toxicity: a specific and sensitive yeast bioluminescence assay

et al. 2011

View full text Add to dashboard Cite

We describe a Saccharomyces cerevisiae bioluminescence assay for UV and arsenate in which bacterial luciferase genes are regulated by the promoter of the yeast gene, UFO1. UFO1 encodes the F-box subunit of the Skp1–Cdc53–F-box protein ubiquitin ligase complex and is induced by DNA damage and by arsenate. We engineered the UFO1 promoter into an existing yeast bioreporter that employs human genes for detection of steroid hormone-disrupting compounds in water bodies. Our analysis indicates that use of an endogenous yeast promoter in different mutant backgrounds allows discrimination between different environmental signals. The UFO1-engineered yeast give a robust bioluminescence response to UVB and can be used for evaluating UV protective sunscreens. They are also effective in detecting extremely low concentrations of arsenate, particularly in pdr5Δ mutants that lack a mechanism to extrude toxic chemicals; however, they do not respond to cadmium or mercury. Combined use of endogenous yeast promoter elements and mutants of stress response pathways may facilitate development of high-specificity yeast bioreporters able to discriminate between closely related chemicals present together in the environment.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Protection Of Cells Irradiated With Uvb By Sunscreen Compoundsmentioning

confidence: 98%

Section: Protection Of Cells Irradiated With Uvb By Sunscreen Compoundsmentioning

confidence: 99%

Section: Protection Of Cells Irradiated With Uvb By Sunscreen Compoundsmentioning

confidence: 99%

See 2 more Smart Citations

UV and arsenate toxicity: a specific and sensitive yeast bioluminescence assay

et al. 2011

View full text Add to dashboard Cite

show abstract

“…Actin was detected using mouse anti-actin clone C-4 (MP Biomedicals Electron microscopy. Electron microscopy was performed as previously described (31).…”

Section: Methodsmentioning

confidence: 99%

Jun Proteins Are Starvation-Regulated Inhibitors of Autophagy

Yogev¹,

Goldberg²,

Anzi³

et al. 2010

Cancer Research

View full text Add to dashboard Cite

The growing number of biological functions affected by autophagy ascribes a special significance to identification of factors regulating it. The activator protein-1 (AP-1) transcription factors are involved in most aspects of cellular proliferation, death, or survival, yet no information regarding their involvement in autophagy is available. Here, we show that the AP-1 proteins JunB and c-Jun, but not JunD, c-Fos, or Fra-1, inhibit autophagy. JunB inhibits autophagy induced by starvation, overexpression of a short form of ARF (smARF), a potent inducer of autophagy, or even after rapamycin treatment. In agreement, acute repression of JunB expression, by JunB knockdown, potently induces autophagy. As expected from autophagy-inhibiting proteins, Jun B and c-Jun expression is reduced by starvation. Decrease in JunB mRNA expression and posttranscriptional events downregulate JunB protein expression after starvation. The inhibition of autophagy by JunB is not mediated by mammalian target of rapamycin (mTOR) regulation, as it occurs also in the absence of mTOR activity, and autophagy induced by JunB knockdown is not correlated with changes in mTOR activity. Nevertheless, the transcriptional activities of c-Jun and JunB are required for autophagy inhibition, and JunB incapable of heterodimerizing is a less effective inhibitor of autophagy. Most importantly, inhibition of autophagy in starved HeLa cells by JunB enhances apoptotic cell death. We suggest that JunB and c-Jun are regulators of autophagy whose expression responds to autophagy-inducing signals.

show abstract

Nanoparticle platforms for dermal antiaging technologies: Insights in cellular and molecular mechanisms

Bhatia

Kumari

Sharma

et al. 2021

WIREs Nanomed Nanobiotechnol

View full text Add to dashboard Cite

Aging is a continuous process defined by a progressive functional decline in physiological parameters. Skin, being one of the most vulnerable organs, shows early signs of aging which are predominantly affected by intrinsic factors like hormone, gender, mood, enzymes, and genetic predisposition, and extrinsic factors like exposure to radiation, air pollution, and heat. Visible morphological and anatomical changes associated with skin aging occur due to underlying physiological aberrations governed by numerous complex interactions at cellular and subcellular levels. Nanoparticles are perceived as a powerful tool in the cosmeceutical industry both for augmenting the efficacy of existing agents and as a novel standalone therapy. Both organic and inorganic nanoparticles have been extensively investigated in antiaging applications. The use of nanoparticles helps to enhance the activity of antiaging molecules by selectively targeting cellular and molecular pathways. On the other hand, the nanoparticle platforms also gained increasing popularity as the skin protectant against extrinsic factors such as UV radiation and pollutants. This review comprehensively discusses skin aging and its mechanism by highlighting the impact on cellular, subcellular, and epigenetic elements. Importantly, the review elaborates on the examples of organic and inorganic nanoparticle-based formulations developed for antiaging application and provides mechanistic insights on how they modulate the mechanisms of skin aging. The clinical progress of nanoparticle antiaging technologies and factors that impact clinical translation are also explored.

show abstract

Aged keratinocyte phenotyping: Morphology, biochemical markers and effects of Dead Sea minerals

Cited by 54 publications

References 46 publications

UV and arsenate toxicity: a specific and sensitive yeast bioluminescence assay

UV and arsenate toxicity: a specific and sensitive yeast bioluminescence assay

Jun Proteins Are Starvation-Regulated Inhibitors of Autophagy

Nanoparticle platforms for dermal antiaging technologies: Insights in cellular and molecular mechanisms

Contact Info

Product

Resources

About