Aged Ripe Pu-erh Tea Reduced Oxidative Stress-Mediated Inflammation in Dextran Sulfate Sodium-Induced Colitis Mice by Regulating Intestinal Microbes

Abstract

Ripened pu-erh tea has the biological activity of antioxidation and anti-inflammation, which inhibits the related parameters of colitis. However, the role of storage-induced changes in bioactive ingredients of ripened pu-erh tea in colitis remains unclear. In this study, 3.5% dextran sulfate sodium-induced colitis mice were treated with 10 mg/kg bw/day extracts, aged 14 years (P2006) and unaged (P2020) ripened pu-erh tea, respectively, for 1 week. We found that ripened pu-erh tea, especially P2006, inhibited the intestinal oxidative stress-mediated inflammation pathway (TLR4/MyD88/ROS/p38MAPK/NF-κB p65), upregulated the expression of intestinal tight junction proteins (Mucin-2, ZO-1, occludin), promoted M2 polarization of macrophages, and in turn, improved the intestinal immune barrier, which stemmed from the reshaping of intestinal microbiota (e.g., increased Lachnospiraceae_NK4A136_group and Akkermansia levels). Our results speculate that drinking aged ripe pu-erh tea (10 mg/kg bw/ day in mice, a human equivalent dose of 7 g/60 kg bw/day) has a practical effect on alleviating and preventing the development of intestinal inflammation.

Theabrownin Alleviates Colorectal Tumorigenesis in Murine AOM/DSS Model via PI3K/Akt/mTOR Pathway Suppression and Gut Microbiota Modulation

“…Meanwhile, TB elevated the Prevotellaceae family and Alloprevotella genus level in rats which consumed a high-fat and high-sugar diet [ 31 , 53 ]. Our data aligns with previous studies that the administration of TB enriched SCFA producing Prevotellaceae family and Alloprevotella genus in the AOM/DSS model [ 54 ]. Short chain fatty acids (SCFA) in the gut, mainly consisting of acetate, propionate, and butyrate, have shown regulatory effects on CRC by alleviating colon inflammation, strengthening the intestinal barrier and regulating the survival of both normal and cancerous colon cells [ 55 , 56 , 57 ].…”

Hawk Tea Flavonoids as Natural Hepatoprotective Agents Alleviate Acute Liver Damage by Reshaping the Intestinal Microbiota and Modulating the Nrf2 and NF-κB Signaling Pathways

“…It seems a there is a possible involvement of the gut-liver axis through the LPS/TLR-4/pro-inflammatory cytokines axis in the pathogenesis of inflammatory processes. Our lab's early studies have shown that the reduction of intestinal inflammation and liver toxicity by tea (Camella sinensis) and hawk tea (Litsea coreana) is due to the reshaping of gut microbes [17,23,54,84], which further supported the finding reported in this paper that the ALD-mediated intestinal microorganism composition affects intestinal and liver inflammation. The intestinal-flora-disorder-mediated activation of TLR4 signaling pathways aggravates lipid metabolic disorders, further causing liver lipid deposition.…”

“…The process of Western blot analysis refers to previous research methods of our laboratory [23]. The liver tissue of mice was washed with cold phosphate buffer solution (PBS) and then treated with a RIPA buffer (RIPA; Servicebio, Wuhan, China) containing a protease inhibitor cocktail.…”

The underlying mechanism of A‐type procyanidins from peanut skin on DSS ‐induced ulcerative colitis mice by regulating gut microbiota and metabolism

“…Besides, Roseburia can regulate the immune system of individuals, and decreases in UC patients (Zhang et al, 2017). Lachnospiraceae has been well recognized as a producer of SCFAs and an inducer of regulatory T cells (Yilmaz & Juillerat, 2019) and Lachnospiraceae_NK4A136_group is an SFCAs-producing bacteria as well, which is related to butyric acid production (Hu et al, 2021). In addition to serving as a source of nutrition for intestinal epithelial cells, SCFAs can also increase the expression of MUC2 and restore the protective effect of the mucus layer against external injury (Buisinea et al, 2001).…”