Ageing, but not yet senescent, rats exhibit reduced muscle quality and sarcoplasmic reticulum function

Russ, David W.; Grandy, Jessica S.; Toma, Kumika; Ward, Christopher W.

doi:10.1111/j.1748-1716.2010.02191.x

Cited by 56 publications

(64 citation statements)

References 70 publications

(130 reference statements)

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“…The rats in our study comprised two age groups (all males): younger adult (6-8months, N5, 417±48.4g) and early-stage aging (24months, N5, 578±10.6g). A 24-month-old rat in this strain is physiologically similar to a 55-to 60-year-old human, and muscle mass and fiber number is reported to be statistically similar to muscles of young adult rats (Russ et al, 2011). Significant signs of sarcopenia in the Fischer 344 ϫ Brown Norway rats do not fully manifest until 33months of age (Lushaj et al, 2008).…”

Section: Materials and Methods Animalsmentioning

confidence: 96%

“…Force divided by muscle mass is used to measure changes in muscle force isolated from changes in muscle size and is referred to as muscle quality (e.g. Dormer et al, 2009;Kan et al, 2005;Russ et al, 2011). We used muscle mass as our denominator because we tested the plantar flexor muscles as a functional group, and thus we could not determine the optimal length for producing force for individual muscles or estimate physiological cross-sectional area (PCSA).…”

Section: Muscle Forcesmentioning

confidence: 99%

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Effects of early-stage aging on locomotor dynamics and hindlimb muscle force production in the rat

Hörner

Russ

Biknevicius

2011

Journal of Experimental Biology

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SUMMARYAttenuation of locomotor function is common in many species of animals as they age. Dysfunctions may emerge from a constellation of age-related impairments, including increased joint stiffness, reduced ability to repair muscle tissue, and decreasing fine motor control capabilities. Any or all of these factors may contribute to gait abnormalities and substantially limit an animal's speed and mobility. In this study we examined the effects of aging on whole-animal locomotor performance and hindlimb muscle mechanics in young adult rats aged 6-8months and 'early aged' 24-month-old rats (Rattus norvegicus, Fischer 344 ϫ Brown Norway crosses). Analyses of gaits and kinematics demonstrated that aged rats moved significantly more slowly, sustained longer hindlimb support durations, moved with a greater proportion of asymmetrical gaits, were more plantigrade, and moved with a more kyphotic spinal posture than the young rats. Additionally, the external mechanical energy profiles of the aged animals were variable across trials, whereas the younger rats moved predominantly with bouncing mechanics. In situ analyses of the ankle extensor/plantar flexor muscle group (soleus, plantaris, and medial and lateral gastrocnemii) revealed reduced maximum force generation with aging, despite minimal changes in muscle mass. The weakened muscles were implicated in the degradation of hindfoot posture, as well as variability in center-of-mass mechanics. These results demonstrate that the early stages of aging have consequences for whole-body performance, even before age-related loss of muscle mass begins.

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Section: Materials and Methods Animalsmentioning

confidence: 96%

Section: Muscle Forcesmentioning

confidence: 99%

Effects of early-stage aging on locomotor dynamics and hindlimb muscle force production in the rat

Hörner

Russ

Biknevicius

2011

Journal of Experimental Biology

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“…Many reports focus on alterations to the intrinsic properties of single muscle fibers to explain changes in muscle quality (MQ) (Canepari et al 2010;Russ et al 2011) and others recognize that much of this change is secondary to declines in the number of functioning motor units (MUs), one factor leading to fiber atrophy (Larsson 1995;Lexell et al 1988). The relationship between intrinsic contractile changes and extrinsic neuroanatomical factors is not fully appreciated and few studies have attempted to assess both aspects in concert (Campbell et al 1973;Lexell et al 1988).…”

Section: Introductionmentioning

confidence: 99%

The influence on sarcopenia of muscle quality and quantity derived from magnetic resonance imaging and neuromuscular properties

Power

Allen

Booth

et al. 2014

AGE

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The relative contributions of intrinsic and extrinsic neuromuscular factors on sarcopenia are poorly understood. The associations among age-related declines of strength, muscle mass, and muscle quality in response to motor unit (MU) loss have not been systematically investigated in the same groups of subjects. The purpose was to assess MU loss, MRI-derived muscle cross-sectional area (CSA), muscle protein quantity (MPQ), and normalized strength of the dorsiflexors in one group of young (~25 years) adult males compared with two groups of healthy men aged 60-85 years. Muscle strength was assessed on a dynamometer and was~25 % lower in both older groups, but CSA was less only in the older (>75 years) men, with no differences between the young and old (60-73 years). Normalized strength tended to be lower in both groups of aged men compared to young. For MPQ, only the older men showed~8 % lower values than the young and old men. Older men had fewer functioning MUs than old, and both groups of aged men had fewer MUs than young men. Muscle quality appears to be maintained in the old likely due to compensatory MU remodeling, but in the older group (>75 years), MU loss was higher and MPQ was lower.

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“…Dysregulation of skeletal muscle [Ca 2 + ] i has been implicated in the deleterious changes in muscle function with aging (Delbono et al, 1995;Wang et al, 2000;Gonzalez et al, 2003;Ryall et al, 2004;Schertzer et al, 2005;Weisleder et al, 2006;Russ et al, 2011). PV is a high-affinity, slow buffer of Ca 2 + , whose expression decreases in rat fasttwitch skeletal muscle with aging (by 63% in EDL muscles from 8-month-old rats compared with 24-month-old rats) (Cai et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

“…One of the contributing factors is that the loss of muscle mass in aging is preceded by a slowing in the maximal rate of relaxation of the twitch and tetanus (Ryall et al, 2004;Schertzer et al, 2005;Russ et al, 2011) and a reduction in both peak tetanic intracellular Ca 2 + concentration ([Ca 2 + ] i ) and maximal tetanic force (Gonzalez et al, 2003;Ryall et al, 2004;Schertzer et al, 2005;Russ et al, 2011). The reduced peak tetanic [Ca 2 + ] i is attributed to an elevated resting [Ca 2 + ] i , which is 25% and 74% higher in soleus and extensor digitorum longus (EDL) muscles from old (27-30 months) compared with young rats (4-6 months), respectively (Fraysse et al, 2006).…”

mentioning

confidence: 99%

Parvalbumin Gene Transfer Impairs Skeletal Muscle Contractility in Old Mice

et al. 2012

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Sarcopenia is the progressive age-related loss of skeletal muscle mass associated with functional impairments that reduce mobility and quality of life. Overt muscle wasting with sarcopenia is usually preceded by a slowing of the rate of relaxation and a reduction in maximum force production. Parvalbumin (PV) is a cytosolic Ca 2 + buffer thought to facilitate relaxation in muscle. We tested the hypothesis that restoration of PV levels in muscles of old mice would increase the magnitude and hasten relaxation of submaximal and maximal force responses. The tibialis anterior (TA) muscles of young (6 month), adult (13 month), and old (26 month) C57BL/6 mice received electroporation-assisted gene transfer of plasmid encoding PV or empty plasmid (pcDNA3.1). Contractile properties of TA muscles were assessed in situ 14 days after transfer. In old mice, muscles with increased PV expression had a 40% slower rate of tetanic force development ( p < 0.01), and maximum twitch and tetanic force were 22% and 16% lower than control values, respectively ( p < 0.05). Muscles with increased PV expression from old mice had an 18% lower maximum specific (normalized) force than controls, and absolute force was *26% lower at higher stimulation frequencies (150-300 Hz, p < 0.05). In contrast, there was no effect of increased PV expression on TA muscle contractile properties in young and adult mice. The impairments in skeletal muscle function in old mice argue against PV overexpression as a therapeutic strategy for ameliorating aspects of contractile dysfunction with sarcopenia and help clarify directions for therapeutic interventions for age-related changes in skeletal muscle structure and function.

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Ageing, but not yet senescent, rats exhibit reduced muscle quality and sarcoplasmic reticulum function

Abstract: These data suggest a possible role for impaired SR Ca(2+) release in ageing-related loss of muscle quality, although not through loss of RYR expression.

Cited by 56 publications

References 70 publications

Effects of early-stage aging on locomotor dynamics and hindlimb muscle force production in the rat

Effects of early-stage aging on locomotor dynamics and hindlimb muscle force production in the rat

The influence on sarcopenia of muscle quality and quantity derived from magnetic resonance imaging and neuromuscular properties

Parvalbumin Gene Transfer Impairs Skeletal Muscle Contractility in Old Mice

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