Ageing Induced Hyperproduction of Reactive Oxygen Species and Dysbalance in Enzymatic Link of Antioxidant Defense System of Skin and Therapeutic Efficacy of Artichoke Extract

Abstract

Skin aging is multitarget persistence processing that immediately involve hyperproduction of free radicals under influence of intrinsic and extrinsic factors and deterioration in intimal antioxidant defense system. The goal of the study was the evaluation of the anti-oxidant potential of artichoke standartizated extracts, 2%, as a protective strategy against skin age-associated oxidative damage caused by D-galactose (D-gal) in rats. 58 female Wistar rats included in the experimental design. D-gal-induced aging was reproduced in 36 animals of main group, and 12 rats included in control group. All animals in main group were randomized for 3 groups: I – animals with skin aging reproduced model receive saline, II – animals with skin aging rats receive artichoke extracts (with content of chloroagenic acid 2.0%) in a dose of intradermal injection 0.13 mg/kg and main III group - animals with skin aging receive 1.3 mg/kg artichoke extract twice at weeks during 4 weeks. Influence of artichoke extracts restores skin relative weight and leads to decreasing the rate of generation of superoxide anion, hydrogen peroxide and lipid peroxidation (LPx), increasing activity of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and reverse ratio SOD/(catalase+GPx) to the production of H2O2 from superoxide dismutation coupling with the decrease ratio of generated O2-/H2O2. Low-dose of intradermally microinjection of artichoke extracts, 2%, activated the enzymatic link in innate antioxidant defense system in D-gal-induced skin aging model and could be recommended for applications in cosmetics as antiaging mesotherapy.

Vegetables and Their Bioactive Compounds as Anti-Aging Drugs

“…In addition, Sukoyan et al [ 151 ] evaluated the antioxidant potential of standardized artichoke extracts (2%) as a protective strategy against skin age-associated oxidative damage caused by d -galactose in rats. These researchers discovered that a low dose of artichoke extracts (2%), by intradermal microinjection, triggers the enzymatic link in the innate antioxidant defense system in a skin aging model, thus suggesting its use in cosmetics as anti-aging mesotherapy.…”

Functional Status of Magallana Gigas Infected by Pione Vastifica

“…С другой стороны, избыточное производство АФК может привести к повреждению внутриклеточных структур организма [18]. В ответ на гиперпродукцию АФК в организме активизируется антиоксидантная система защиты, которая инактивирует свободные радикалы [19,20]. Одним из основных ферментов, участвующих в каскаде антиоксидантных реакций, является каталаза (КАТ) [21].…”