Agglutinating and Precipitating Capacity of Rabbit Anti-Salmonella typhosaγG and γM Antibodies During Prolonged Immunization

Abstract

Agglutinating and precipitating capacity of rabbit anti-Salmonella typhosa yG and yM antibodies during prolonged immunization. J. Bacteriol. 92:880-886. 1966.-Antibody produced in rabbits immunized with acetone-dried typhoid bacilli was followed over a period of 445 days by agglutination and by quantitative precipitation. Repeated injections of vaccine resulted in suppression of antibody titers. Both-yG and yM antibodies were rapidly increased by booster injections after rest periods during which titers had decreased to low levels. The 0 agglutinin titers and the amount of antibody protein, as determined by precipitation with endotoxin, generally were parallel, except in serum specimens in which unusually large proportions of the agglutinating activity were found in the yG fraction. These exceptions were explained by the greater agglutinating capacity of the-yM. Endotoxin precipitated about 10 times as much antibody from-yG preparations as it did from yM fractions of equivalent agglutinating strength. A much higher proportion of the serological

Serological Properties of γG and γM Antibodies to the Somatic Antigen of Vibrio cholerae During the Course of Immunization of Rabbits

“…Although the somatic antigens of gram-negative bacilli elicit an immune response which appears to be predominantly yM, a considerable proportion of the antibody activity may be found in the yG fraction of serum (7, 22, 23, 26). The apparent distribution of antibody activity among the immunoglobulins depends to a large extent on the serological procedure used (21,25). Thus, in the present study, the greater portion of the activity of late sera (pools 4, 5, and 6) as measured by CF appeared to be in the -yG fractions, although the agglutinating and vibriocidal activities were greater in the yM fractions ( Fig.…”

The Protective Effect against E. coli of O and K Antibodies of Different Immunoglobulin Classes

“…This is illustrated in Table I, where it can be seen that the agglutinating antibodies are mainly of the IgM class in contrast to the precipitating antibodies which are predominantly of the IgG class. In relation to the amount of antibody, however, it has been reported that for detectable agglutination, some 20 times more IgG than IgM antibodies appears to he needed (15,17). If these estimations are valid our results point to a similar protective effect of the 19S (IgM) and 7S (IgG) antibodies.…”

Regulatory Effect of Specific Antibody on Anti‐Bacterial Antibody Response to Salmonella in Mice

“…This has been regarded as a variety of the "feedback mechanism ," in which specific 7S antibody suppresses further synthesis of 19S antibody [10]. In the immune response to the somatic antigens of salmonella, a single injection of the chrome-vaccine [1] prepared from salmonella or multiple injections with killed salmonella organisms elicits predominantly 19S antibody formation in animals, though a small amount of 7S antibody may appear later [5,6,8,9]. The production of 19S antibody appears to continue for a long time without being terminated by the presence of 7S antibody [7,8].…”

“…In the immune response to the somatic antigens of salmonella, a single injection of the chrome-vaccine [1] prepared from salmonella or multiple injections with killed salmonella organisms elicits predominantly 19S antibody formation in animals, though a small amount of 7S antibody may appear later [5,6,8,9]. The production of 19S antibody appears to continue for a long time without being terminated by the presence of 7S antibody [7,8]. To investigate this rather peculiar pattern of antibody formation to salmonella 0 antigens, we examined whether specific antibodies, passively administered, suppressed further antibody production to these salmonella O antigens in mice.…”