Agglutination of Bacterial Cells ofClostridium innocuum, Bifidobacterium longum, andMicrococcus luteusby Lactoferrin and Ovotransferrin

Binding of Lactoferrin to Bacterial Cells of theClostridiumSpecies and Their Agglutination

“…These sites are also included in lactoferricin that had strong bactericidal action and was isolated from a pepsin-treated hydrolysate of Lf.25) On the other hand, Lf, which chemically modified the guanidino groups of arginine residues or the e-amino groups of lysine residues could not cause the agglutination of C. innocuum cells. 7) In the case of the C. pei:fringens strain, it seems that these residues of the Lf molecules may have participated in binding to the cell surface of the bacteria. Several workers have reported that binding proteins specific to Lf existed on the cell surface of such bacterial strains as E. coli,26) IVleisseria meningitidts,2') and Stuphylococcus auretLs.28-29} Further research is needed to identify whether a specific binding receptor to bLf exists on the cell surface of C, pei:t}'ingens and other strains.…”

Characterization of the Protein and Glycan Moieties in Different Forms of Bovine Lactoferrin

“…28) The glycans in lactoferrin have also been shown to play an important role in the agglutination of bacterial cells. 8) This agglutination effect of LF -a is presumed to be stronger than that of LF-b, because the content of glycans in the former was higher than that in the latter. However, the difference in biological functions between LFs-a and -b is the next aspect to be clarified.…”

Glycans of bovine lactoferrin function as receptors for the type 1 fimbrial lectin of Escherichia coli

“…These results suggest that the polypeptide chain of bLF as well as its oligomannoside-type glycans may have a role in bacterial agglutination. Several studies have demonstrated that LF agglutinates certain bacteria (17,18,25). In the case of E. coli, this phenomenon has been observed only in the presence of lysozyme (21).…”