Aggregation-Induced Electrochemiluminescence Based on a Zinc-Based Metal–Organic Framework and a Double Quencher Au@UiO-66-NH₂ for the Sensitive Detection of Amyloid β 42 via Resonance Energy Transfer

Abstract

A novel sandwich electrochemiluminescence (ECL) biosensor based on aggregation-induced electrochemiluminescence resonance energy transfer (AIECL-RET) was designed for the sensitive detection of amyloid β42 (Aβ42). The synthesized silver nanoparticle-functionalized zinc metal−organic framework (Ag@ ZnPTC) and gold nanoparticle-functionalized zirconium organic framework (Au@UiO-66-NH 2 ) were used as the ECL donor and acceptor, respectively. AgNPs were generated in situ on the surface of ZnPTC, which further improved the ECL intensity and the loading of antibody 1 (Ab1). Under the optimized experimental conditions, the linear detection range of Aβ42 concentration was 10 fg/mL to 100 ng/mL, and the detection limit was 2.4 fg/mL (S/N = 3). The recoveries of Aβ42 were 99.5−104%. The method has good stability, repeatability, and specificity.

Synergistic Multieffect Catalytic Amplified Cathodic Electrochemiluminescence Biosensor via Target Binding-Induced Aptamer Conformational Changes for the Ultrasensitive Detection of Synthetic Cathinone

“…Following that, investigation of the electron paramagnetic resonance (EPR) then allowed us to demonstrate the presence of OH · in the reaction system. Herein, the 5,5-dimethyl-1-pyrroline N -oxide (DMPO) free-radical trapping agent was used to analyze the EPR spectrum of the Ag@CuCo 2 O 4 /PTCA-COF/S 2 O 8 2– system. , As observed in Figure D, it was a typical quadruple peak with a peak intensity ratio of 1:2:2:1, which corresponded to the DMPO–OH adducts and confirmed the production of hydroxyl radicals (OH · ). Meanwhile, between the four above-mentioned typical peaks, there were two additional smaller peaks that were indicative of DMPO–SO 4 adducts and showed the existence of SO 4 ·– .…”

Amyloid detection in neurodegenerative diseases using MOFs

“…37 These sensors comprise two layers, with one or both layers potentially containing an MOF. 78 Layer 1 is constructed by coating a glass carbon electrode surface with either a modified graphitic carbon nitride nanosheet (g-C 3 N 4 ) or an MOF functionalized with a specific aptamer (Apt1) or antibody (Ab 1 ) targeting the specific amyloid of interest. 79 Subsequently, the target protein, peptide, or its oligomer binds to layer 1.…”

Silver Nanoparticles In Situ Enhanced Electrochemiluminescence of the Porphyrin Organic Matrix for Highly Sensitive and Rapid Monitoring of Tetracycline Residues

“…Silver nanoparticles (Ag NPs) have been reported to be an ideal co-reaction accelerator. They can significantly boost the reaction efficiency of S 2 O 8 2– and the yield of SO 4 •– , and then they can enhance the ECL emission of emitters . Yuan’s group used Ag NPs as the co-reaction accelerator for increasing the ECL intensity of 1-pyrenecarboxaldehyde dots .…”

“…They can significantly boost the reaction efficiency of S 2 O 8 2− and the yield of SO , and then they can enhance the ECL emission of emitters. 19 Yuan's group used Ag NPs as the co-reaction accelerator for increasing the ECL intensity of 1-pyrenecarboxaldehyde dots. 20 Chen and co-workers synthesized Ag NPs as dual-regulating co-reaction accelerators to improve the bipolar ECL emissions of iridium(III) nanorods.…”