Aggregation-Induced Electrochemiluminescence Frame of Silica-Confined Tetraphenylethylene Derivative Matrixes for CD44 Detection via Peptide Recognition

Abstract

The theory of aggregation-induced electrochemiluminescence (AIECL) has introduced new vitality into preparing new electrochemiluminescence (ECL) emitters. However, the progress in the application of biosensing analysis has been slow owing to the lack of AIECL-based functional nanomaterials. Herein, a biosensor was fabricated using mesoporous silica nanosphere (MSN) matrix-confined 1,1,2,2-tetra(4carboxylphenyl)ethylene (TPE) as a well-ordered ECL emitter and selfdesigned WHPWSYC (WC-7) heptapeptide as the target capturer for CD44 detection. TPE and its co-reactant, triethylamine (TEA), were encapsulated in the MSN nanomatrix to enhance the radiation transition by limiting the intramolecular rotation of TPE molecule benefit from the spatial confinement effect, and the ECL intensity is self-enhanced by replacing electron free diffusion in the conventional ECL system. MSN−TPE−TEA can act as satisfactory sensing substrates that improve the reproducibility and batch-to-batch consistency of biosensors and functions as a stable signal label for trace analysis of biomarkers. As a substitute for antibody and hyaluronic acid, the WC-7 heptapeptide significantly reduced the steric hindrance of the sensing interface in CD44 affinity tests. Combined with the DNA strand displacement reaction, this strategy shows a good ECL response to standard CD44 antigen and MCF-7 cells with different concentrations, which is another feasible method for detecting CD44 in body fluids or living cells.

Screening-Capture-Integrated Electrochemiluminescent Aptasensor Based on Mesoporous Silica Nanochannels for the Ultrasensitive Detection of Deoxynivalenol in Wheat

“…Mesoporous silica nanomaterials (MSNMs) are porous materials that are widely used in the field of biosensing because of their stable physical and chemical properties and large specific surface area. , A common strategy for constructing MSNM-based ECL sensors is to employ MSNMs as porous carriers to wrap ECL emitters and use biological materials, such as aptamers (Apts) and antibodies, for hole sealing such that only specific target molecules can recognize them, triggering the inhibition or release of ECL emitters, which is recorded as the signal output changes. , Hong et al loaded tris­(2,2′-bipyridyl)­ruthenium­(II) (Ru­(bpy) 3 2+ ) into spherical MSNMs and attached the troponin I (TnI) antibody to their surface for the specific recognition of TnI . When Ru­(bpy) 3 2+ was loaded inside the MSNMs and reacted with tripropylamine in the electrolyte, a strong ECL signal was observed.…”

Developing a Hetero-Trinuclear Erbium(III)–Copper(II) Complex Based on Apoferritin: Targeted Photoacoustic Imaging and Multimodality Therapy of Tumor

“…Cancer is one of the major threats to human health with the highest mortality worldwide of all causes of death because it remains challenging to accurately diagnose and effectively treat, − leading to cancer progression. To solve these problems, theranostics that integrate diagnostic and therapeutic moieties have been extensively explored. , To date, the vast majority of theranostics are realized by independent diagnosis and therapeutic agents, which not only lead to discrepant pharmacokinetics, selectivity, and biodistribution between different agents but also produce uncertain systemic cytotoxicity. , Single-molecule theranostic agents were shown to be a promising strategy − that avoided the drawbacks of independent diagnostic and therapeutic agents. − …”

Nickel Single-Atom Catalyst Boosts Electrochemiluminescence of Graphitic Carbon Nitride for Sensitive Detection of HBV DNA

“…Electrochemiluminescence (ECL) is a redox-triggered luminescent process . The unique luminescence mechanism enables ECL technology to exhibit high sensitivity and near-zero background signal, thus being extensively applied in the biosensing field. − ECL luminophore is an essential player in the construction of ECL biosensors. Among the various ECL luminophores that have been developed, graphitic carbon nitride (g-C 3 N 4 ) has aroused considerable attention because of its low toxicity, good biocompatibility, and adjustable luminescence characteristics. − Since Cheng et al first reported the ECL property of g-C 3 N 4 , various g-C 3 N 4 -based nanocomposites and vacancy-modified g-C 3 N 4 have sprung up and been successfully used to construct ECL sensors. − These g-C 3 N 4 -based ECL sensing platforms mainly relied on their cathode ECL emission with peroxydisulfate (S 2 O 8 2– ) as coreactant.…”