Aggregation induced nucleic acids recognition by homodimeric asymmetric monomethyne cyanine fluorochromes in mesenchymal stem cells

Ishkitiev, Nikolay; Miteva, Marina; Micheva, Maria; Stoyanova, Tanya; Lozanova, Vesela V.; Lozanov, Valentin; Mihaylova, Zornitsa; Cheshmedzhieva, Diana; Kandinska, Meglena I.; Rangelov, Miroslav; Todorova, Nadezhda; Ilieva, Sylvia; Baluschev, Stanislav; Gargallo, Raimundo; Calenic, Bogdan; Constantinescu, Ileana; Landfester, Katharina; Vasilev, Aleksey

doi:10.1016/j.ijbiomac.2023.126094

Cited by 3 publications

(5 citation statements)

References 38 publications

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“…TO is an established strong intercalator. T1 dye has also been proven as an intercalator using CD spectroscopy and fluorescence titration in our previous work [ 24 ]. The change in fluorescence of the T1-DNA complex upon addition of TO is shown in Figure 5 .…”

Section: Resultsmentioning

confidence: 99%

Asymmetric Monomethine Cyanine Dyes with Hydrophobic Functionalities for Fluorescent Intercalator Displacement Assay

Ilieva,

Bozova,

Rangelov

et al. 2023

Molecules

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A new green procedure has been applied for the synthesis and purification of asymmetric monomethine cyanine dyes. The photophysical properties of the newly synthesized compounds have been examined by combined application of spectroscopic and theoretical methods. The structural characteristics of the molecules and dimer formation were characterized by quantum chemical computation and juxtaposed to the aggregachromism in UV/Vis spectra. The applicability of the dyes as fluorogenic nucleic acid probes has been proven by fluorescence titration, and their binding constants have been calculated. The mode of ligand–dsDNA/RNA interaction was rationalized by means of CD spectroscopy, molecular docking analysis, and fluorescent intercalator displacement experiments.

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Section: Resultsmentioning

confidence: 99%

Asymmetric Monomethine Cyanine Dyes with Hydrophobic Functionalities for Fluorescent Intercalator Displacement Assay

Ilieva,

Bozova,

Rangelov

et al. 2023

Molecules

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“…MCDs are also used in real-time PCR analysis of nucleic acids [ 12 , 13 , 14 , 15 ]. A wide range of biological and biomedical systems currently being studied with the use of spectral fluorescent probes stimulates a permanent emergence of research aimed at developing new efficient and selective dye probes for the detection and staining of biomolecules in such systems (see, e.g., [ 16 , 17 , 18 ]). There are a large number of works by different research groups devoted to the synthesis and spectral fluorescent study of various MCDs (symmetrical and unsymmetrical) in order to develop probes for nucleic acids [ 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 ].…”

Section: Introductionmentioning

confidence: 99%

“…A wide range of biological and biomedical systems currently being studied with the use of spectral fluorescent probes stimulates a permanent emergence of research aimed at developing new efficient and selective dye probes for the detection and staining of biomolecules in such systems (see, e.g., [ 16 , 17 , 18 ]). There are a large number of works by different research groups devoted to the synthesis and spectral fluorescent study of various MCDs (symmetrical and unsymmetrical) in order to develop probes for nucleic acids [ 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 ]. There are also some works directed to the study of primary photophysical and photochemical processes in MCD molecules, both free and noncovalently bound to nucleic acids [ 35 , 36 , 37 , 38 , 39 , 40 ] (see also the review [ 41 ]).…”

Section: Introductionmentioning

confidence: 99%

“…Sci. 2023, 24, x FOR PEER REVIEW 2 of 27 molecules in such systems (see, e.g., [16][17][18]). There are a large number of works by different research groups devoted to the synthesis and spectral fluorescent study of various MCDs (symmetrical and unsymmetrical) in order to develop probes for nucleic acids [16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34].…”

Section: Introductionmentioning

confidence: 99%

“…2023, 24, x FOR PEER REVIEW 2 of 27 molecules in such systems (see, e.g., [16][17][18]). There are a large number of works by different research groups devoted to the synthesis and spectral fluorescent study of various MCDs (symmetrical and unsymmetrical) in order to develop probes for nucleic acids [16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34]. There are also some works directed to the study of primary photophysical and photochemical processes in MCD molecules, both free and noncovalently bound to nucleic acids [35][36][37][38][39][40] (see also the review [41]).…”

Section: Introductionmentioning

confidence: 99%

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Photonics of Some Monomethine Cyanine Dyes in Solutions and in Complexes with Biomolecules

Pronkin,

Tatikolov

2023

IJMS

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In search of new probes for biomolecules, the spectral fluorescent study of four monomethine cyanine dyes (MCD), both unsymmetrical and symmetrical, has been carried out in different organic solvents, in aqueous buffer solutions, and in the presence of DNA and HSA. The complexation of MCD with biomacromolecules leads to a steep growth of the fluorescence intensity. Complexes of MCD with dsDNA and HSA of various types were modeled in silico by molecular docking. Experiments on thermal dissociation of dsDNA in the presence of MCD showed the formation of intercalative complexes of MCD with DNA. Quenching of intrinsic fluorescence of HSA by MCD occurred with rate constants much higher than the diffusion limit, that is, in dye–HSA complexes. Effective constants of MCD complexation with the biomacromolecules were estimated. MCD 1 has the best characteristics as a possible fluorescent probe for dsDNA and can serve as a sensitive and selective probe for dsDNA in the presence of HSA. Photochemical properties of MCD complexed with DNA have been also studied. An increase in the quantum yield of the triplet states of MCD in complexes with DNA has been found, which may be important for using these dyes as potential candidates in photodynamic therapy.

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Quinolino[1,2-a]quinolinium bromide and isoquinolino[2,1-a]quinolinium bromide derivatives as DNA ligands and photocytotoxic agents

Perevozchikova,

Chernikova,

Fedorov

et al. 2025

Journal of Photochemistry and Photobiology A: Chemistry

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Aggregation induced nucleic acids recognition by homodimeric asymmetric monomethyne cyanine fluorochromes in mesenchymal stem cells

Cited by 3 publications

References 38 publications

Asymmetric Monomethine Cyanine Dyes with Hydrophobic Functionalities for Fluorescent Intercalator Displacement Assay

Asymmetric Monomethine Cyanine Dyes with Hydrophobic Functionalities for Fluorescent Intercalator Displacement Assay

Photonics of Some Monomethine Cyanine Dyes in Solutions and in Complexes with Biomolecules

Quinolino[1,2-a]quinolinium bromide and isoquinolino[2,1-a]quinolinium bromide derivatives as DNA ligands and photocytotoxic agents

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