Aggregation of red blood cells studied by ultrasound backscattering

Boynard, M.; Lelièvre, Jean-Claude; Guillet, R.

doi:10.3233/bir-1987-24503

Cited by 38 publications

(10 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…26,27 Due to its importance in hemorheology, the RBC aggregation process has many approaches. Measurement of optical microscopy, 28 zeta sedimentation rate, 29 image analysis in a flow chamber, 30 ultrasound backscattering, 31,32 and photometry [19][20][21][22]33 are among the methods that have been employed to quantitatively study the aggregation. Despite the fact that RBC aggregation can be assessed using different techniques, two main approaches have been developed in the aggregometry.…”

Section: Introductionmentioning

confidence: 99%

Aggregation of red blood cells in suspension: study by light-scattering technique at small angles

Pop

Neamţu

2008

J. Biomed. Opt.

View full text Add to dashboard Cite

Red blood cells (RBCs) in the presence of plasma proteins or other macromolecules have a tendency to form aggregates. Light-scattering technique was used to investigate the RBC aggregation process. A highly diluted suspension of RBCs was illuminated with a 632.8-nm HeNe laser. Angular-resolved measurements of light intensity scattered by an RBC suspension from a 200-microm thick optical glass cuvette during 10 min of their aggregation process were performed at 1 to 4 off-axis deg with a very high angular resolution, at hematocrits in the range of 3.5 x 10(-2) to 10(-1). The angular spreading of forward-scattered light at small angles during the RBC aggregation process was described in terms of a new, effective phase function model that has been used for fitting the experimental data. The aggregated RBCs' optical properties, such as effective scattering anisotropy and scattering cross section, were determined. The results were compared with prediction of Mie theory for equivolumetric spherical particles. The time dependence of the aggregates mean radius and of the mean number of cells per aggregate was also calculated. Last, the potential of the proposed technique (forward-scattering light technique) as a new quantitative investigation of cellular aggregation process was estimated.

show abstract

Section: Introductionmentioning

confidence: 99%

Aggregation of red blood cells in suspension: study by light-scattering technique at small angles

Pop

Neamţu

2008

J. Biomed. Opt.

View full text Add to dashboard Cite

show abstract

“…The addition of plasma allowed the formation of SEC, with increased concentrations of fibrinogen facilitating more pronounced SEC, whereas RBC‐depleted blood components, including platelet‐rich plasma, were unable to generate SEC. Other studies have emphasized the importance of hemoglobin concentration or HCT on the development of SEC, in addition to fibrinogen concentration 4,5 …”

Section: Discussionmentioning

confidence: 99%

“…Spontaneous echocardiographic contrast (SEC), or ‘smoke,’ is a described finding in the atrium or atrial appendage of people 1 and cats 2 with significant cardiac disease and hypercoagulability. SEC is described as a dynamic echogenic swirling pattern within cardiac chambers or blood vessels that is distinct from white noise artifact 3–6 . This pattern is thought to represent aggregation of RBCs, with larger aggregates of RBCs increasing the amplitude of ultrasonographic signal backscatter 4,7 …”

Section: Introductionmentioning

confidence: 99%

Spontaneous echocardiographic contrast in three dogs

Ralph¹,

Saunders²,

Hariu³

et al. 2011

J Vet Emergen Crit Care

View full text Add to dashboard Cite

show abstract

“…Erythrocytes, which naturally tend to form rouleaux-type structures, do form, however, more compact clump-type aggregates, whenever inflammatory plasma molecules and conditions affecting their cellular membrane's properties are present [3][4][5]. It has long been demonstrated that ultrasound is sensitive to the presence of RBC aggregation and that the backscattered signal from flowing blood is enhanced with the aggregate size [6][7][8][9]. Quantitative ultrasound (QUS) methods are nowadays well known for their ability to provide non-invasive information on tissue microstructures [10].…”

Section: Introductionmentioning

confidence: 99%

Pilot clinical study of quantitative ultrasound spectroscopy measurements of erythrocyte aggregation within superficial veins

Chayer

Allard

Zhao

et al. 2020

View full text Add to dashboard Cite

BACKGROUND: An enhanced inflammatory response is a trigger to the production of blood macromolecules involved in abnormally high levels of erythrocyte aggregation. OBJECTIVE: This study aimed at demonstrating for the first time the clinical feasibility of a non-invasive ultrasound-based erythrocyte aggregation quantitative measurement method for potential application in critical care medicine. METHODS: Erythrocyte aggregation was evaluated using modeling of the backscatter coefficient with the Structure Factor Size and Attenuation Estimator (SFSAE). SFSAE spectral parameters W (packing factor) and D (mean aggregate diameter) were measured within the antebrachial vein of the forearm and tibial vein of the leg in 50 healthy participants at natural flow and reduced flow controlled by a pressurized bracelet. Blood samples were also collected to measure erythrocyte aggregation ex vivo with an erythroaggregometer (parameter S 10 ). RESULTS: W and D in vivo measurements were positively correlated with the ex vivo S 10 index for both measurement sites and shear rates (correlations between 0.35-0.81, p < 0.05). Measurement at low shear rate was found to increase the sensitivity and reliability of this non-invasive measurement method. CONCLUSIONS: We behold that the SFSAE method presents systemic measures of the erythrocyte aggregation level, since results on upper and lower limbs were highly correlated.

show abstract

Aggregation of red blood cells studied by ultrasound backscattering

Cited by 38 publications

References 0 publications

Aggregation of red blood cells in suspension: study by light-scattering technique at small angles

Aggregation of red blood cells in suspension: study by light-scattering technique at small angles

Spontaneous echocardiographic contrast in three dogs

Pilot clinical study of quantitative ultrasound spectroscopy measurements of erythrocyte aggregation within superficial veins

Contact Info

Product

Resources

About