Aggregation processes in micellar solutions: a Raman study

Cazzolli, Giorgia; Caponi, Silvia; Defant, Andrea; Gambi, C. M. C.; Marchetti, S.; Mattarelli, Maurizio; Montagna, M.; Rossi, Barbara; Rossi, F.; Viliani, G.

doi:10.1002/jrs.4120

Cited by 25 publications

(21 citation statements)

References 31 publications

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“…The ability of Raman spectroscopy to investigate the molecular vibrational modes is well known, and it was applied in different fields in condensed matter physics. [18][19][20][21] Recently reaching the micrometric spatial resolution, thanks to the coupling with microscopes, and due to its non-contact and label free nature, Raman micro-spectroscopy has been applied to analyse the status of biological cells [22][23][24][25][26] and tissues. 27,28 In order to investigate the status of SH-SY5Y cells by Raman spectroscopy, we compare the spectra of cells grown on the PANI polymeric films with those of cells grown on silicon covered with silica layers, a control substrate which assures the good biocompatibility.…”

Section: Resultsmentioning

confidence: 99%

A multidisciplinary approach to study the functional properties of neuron-like cell models constituting a living bio-hybrid system: SH-SY5Y cells adhering to PANI substrate

et al. 2016

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ARTICLES YOU MAY BE INTERESTED INPolysaccarides-based gels and solid-state electronic devices with memresistive properties: Synergy between polyaniline electrochemistry and biology AIP Advances 6, 111302 (2016) A living bio-hybrid system has been successfully implemented. It is constituted by neuroblastic cells, the SH-SY5Y human neuroblastoma cells, adhering to a poly-anyline (PANI) a semiconductor polymer with memristive properties. By a multidisciplinary approach, the biocompatibility of the substrate has been analyzed and the functionality of the adhering cells has been investigated. We found that the PANI films can support the cell adhesion. Moreover, the SH-SY5Y cells were successfully differentiated into neuron-like cells for in vitro applications demonstrating that PANI can also promote cell differentiation. In order to deeply characterize the modifications of the bio-functionality induced by the cell-substrate interaction, the functional properties of the cells have been characterized by electrophysiology and Raman spectroscopy. Our results confirm that the PANI films do not strongly affect the general properties of the cells, ensuring their viability without toxic effects on their physiology. Ascribed to the adhesion process, however, a slight increase of the markers of the cell suffering has been evidenced by Raman spectroscopy and accordingly the electrophysiology shows a reduction at positive stimulations in the cells excitability. © 2016 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license

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Section: Resultsmentioning

confidence: 99%

A multidisciplinary approach to study the functional properties of neuron-like cell models constituting a living bio-hybrid system: SH-SY5Y cells adhering to PANI substrate

et al. 2016

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“…Therefore, ν as (CH 2 ) can be used as an indicator for the SDS self-assembly process [16]. Due to the high spectral intensity, parallel polarized Raman spectra are used in previous Raman spectroscopic studies [2,23]. We also record the parallel polarized Raman spectra of extremely diluted aqueous SDS solution (0. .…”

Section: A Parallel Polarized Raman Spectra Of Sds In Diluted Aqueoumentioning

confidence: 99%

“…The self-assembly process of surfactants is of great significance in many fields. The critical micelle concentration of surfactants (CMC), which refers to the concentration of the surfactant required to form micelles, has been extensively studied [1][2][3][4][5][6][7][8]. Surface tension [9], light scattering [10,11], fluorescence [12], and X-ray scattering [13] can be used to obtain the CMC values.…”

Section: Introductionmentioning

confidence: 99%

“…However, parallel polarized Raman spectra in the C−H stretching region in diluted aqueous solutions are usually overlapped by the O−H stretching Raman bands of water, thus the detection sensitivity is relatively low. As a result, studies on the aggregation process of surfactants in water have been limited to relatively high concentrations [2,23].…”

Section: Introductionmentioning

confidence: 99%

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Intermolecular Interactions in Self-Assembly Process of Sodium Dodecyl Sulfate by Vertically Polarized Raman Spectra

Wang

Lin

Chen

et al. 2017

Chinese Journal of Chemical Physics

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Molecular self-assembly is extremely important in many fields, but the characterization of their corresponding intermolecular interactions is still lacking. The C−H stretching Raman band can reflect the hydrophobic interactions during the self-assembly process of sodium dodecyl sulfate (SDS) in aqueous solutions. However, the Raman spectra in this region are seriously overlapped by the OH stretching band of water. In this work, vertically polarized Raman spectra were used to improve the detection sensitivity of spectra of C−H region for the first time. The spectral results showed that the first critical micelle concentration and the second critical micelle concentration of SDS in water were 8.5 and 69 mmol/L, respectively, which were consistent with the results given by surface tension measurements. Because of the high sensitivity of vertically polarized Raman spectra, the critical micelle concentration of SDS in a relatively high concentration of salt solution could be obtained in our experiment. The two critical concentrations of SDS in 100 mmol/L NaCl solution were recorded to be 1.8 and 16.5 mmol/L, respectively. Through comparing the spectra and surface tension of SDS in water and in NaCl solution, the self-assembly process in bulk phase and at interface were discussed. The interactions among salt ions, SDS and water molecules were also analyzed. These results demonstrated the vertically polarized Raman spectra could be employed to study the self-assembly process of SDS in water.

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“…Changes in lipid membrane structure can be observed by monitoring relative Raman peak intensities that represent intramolecular and intermolecular order, which, in turn, are indicators of lipid phase changes. The methylene C‐C stretching (1030–1150 cm −1 ) and the methylene C‐H stretching (2800–3100 cm −1 ) regions are used as indicators of membrane order, but phase changes can also be detected by observing peak shifts . With a confocal‐Raman microscope, it is possible to optically trap individual liposomes so that Raman scattering is collected from one liposome while minimizing contributions from the surrounding solution .…”

Section: Introductionmentioning

confidence: 99%

Infrared versus light scattering techniques to monitor the gel to liquid crystal phase transition in lipid membranes

Sassi

Caponi

Ricci

et al. 2015

J Raman Spectroscopy

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In this study, Fourier transform infrared, Raman and Brillouin spectroscopy have been used to study lipid phase behavior of hydrated as well as dried multilamellar L-α-phosphatidylcholine assemblies, in order to compare limitations and potentials of the different techniques. Dried lipid samples have been studied in the presence and absence of trehalose, which is known to affect the phase behavior of these systems. The methylene C-H stretching (2800-3000 cm À1 ) region in infrared (IR) and Raman spectra provided mutually consistent information on the rearrangement of lipid acyl chains occurring at the lipid melting temperature. IR spectra have a higher signal-to-noise ratio, thus permitting a more precise evaluation of the melting temperature. In the hydrated lipid samples, the C-H stretching region in the Raman spectra is less affected by the contribution of water compared with that in the IR spectra. Raman spectra are particularly suitable to simultaneously study both lipid and water contributions allowing to distinguish ice from non-frozen water below 0°C. Brillouin light scattering was used to probe the collective dynamics, i.e. the propagation velocity and the attenuation of longitudinal acoustic modes in the lipid samples. Lipid phase transitions are evident from a change in the temperature behavior of the acoustic velocity. Moreover, a strong relaxation process with a characteristic time of 14 ps was observed in the sample dried without trehalose with a maximum in acoustic attenuation at about 45°C, which likely reflects the rearrangement of acyl chains.

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Aggregation processes in micellar solutions: a Raman study

Cited by 25 publications

References 31 publications

A multidisciplinary approach to study the functional properties of neuron-like cell models constituting a living bio-hybrid system: SH-SY5Y cells adhering to PANI substrate

A multidisciplinary approach to study the functional properties of neuron-like cell models constituting a living bio-hybrid system: SH-SY5Y cells adhering to PANI substrate

Intermolecular Interactions in Self-Assembly Process of Sodium Dodecyl Sulfate by Vertically Polarized Raman Spectra

Infrared versus light scattering techniques to monitor the gel to liquid crystal phase transition in lipid membranes

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