Aggressiveness of Xanthomonas axonopodis pv. glycines isolates to soybean and hypersensitivity responses by other plants

Abstract

Xanthomonas axonopodis pv. glycines ( Xag ) causes bacterial pustule disease which can significantly reduce the production of soybean. A collection of 26 isolates of Xag from different soybean-production areas of Thailand was shown to differ with regard to aggressiveness on soybean. They also differed in their ability to induce a hypersensitive response (HR) on four cultivars of tobacco and on other plant species including pepper, tomato, cucumber, pea and sesame. Tomato was most sensitive to HR induction by Xag . Isolate KU-P-34017 caused an HR on all the plant species tested. The minimal concentration of KU-P-34017 needed to induce HR on tobacco was approximately 5 × 10 8 CFU mL − 1 . A bacterium-plant interaction period of at least 2·5 h was necessary for HR, and different temperatures, relative humidity and light periods did not affect HR development. Inhibitors of eukaryotic metabolism, including cobalt chloride, lanthanum chloride and sodium orthovanadate (completely), and cycloheximide (partially) blocked the HR on tobacco, indicating the association of an active plant response. In contrast, the HR on tomato was inhibited only by cobalt chloride.

Evaluation of glyphosate-tolerant soybean cultivars for resistance to bacterial pustule

“…In this regard, variations in pathogenicity have been noted among isolates of X. axonopodis pv. glycines with some being highly virulent to a number of soybean cultivars (Ansari 2005;Kaewnum et al 2005).…”

Soil microbial restoration strategies for promoting climate‐ready prairie ecosystems

“…We expect that the predominance of Gammaproteobacteria in our experiment is because we obtained the newly restored soil from an area with a history of corn/soy rotation. This phylum contains several crop-plant pathogens within the order Xanthomonadales (Kaewnum et al 2005, Korus et al 2017, and this order represented the highest proportion of Gammaproteobacteria in our soils (2.4% on average). Xanthomonadales were more abundant in the one-plant-species treatment than in the three-plant-species treatment.…”

Hemin transported protein of Xanthomonas axonopodis pv. glycines functions on leaf colonization and virulence on soybean

“…glycines strains was assessed on susceptible soybean cv. Spencer following topical spray application (Kaewnum et al, 2005). Briefly, cell suspensions of a given strain (OD600 = 0.2; ca.…”

“…Cell pellets were suspended in sterile water for HR tests on tobacco. HR was assayed as described previously (Kaewnum et al, 2005). Briefly, tobacco plants were inoculated with bacterial suspensions (10 9 cells/ml) by injection of leaf with a syringe.…”

“…Whereas, the extensive genetic and genomic resources are available for X. axonopodis pv. glycines, and it has been the subject of highly productive research centered on the mechanisms of plant host susceptibility/resistance and pathogen virulence and avirulence determinants (Athinuwat et al, 2009;Chatnaparat et al, 2012;Kasem et al, 2007;Kaewnum et al, 2005;Thowthampitak et al, 2008). Recently, the draft genome of X. axonopodis pv.…”