Aging and Atherosclerosis Impair Hematopoietic Stem Cell Function

Abstract

BackgroundStem cell function is compromised with aging, especially when a cardiovascular disease is associated. The aim of this study was to evaluate how aging and atherosclerosis affect bone marrow hematopoietic stem cell (HSC) function in mice.Methods: Male mice were divided into C57 and apoE‐/‐ (2, 12 and 24‐month‐old) groups. Oxidative stress was assessed by 2,7‐dichlorofluorescein (DCF) by flow cytometer. For apoptosis detection, HSC were incubated with Annexin V‐FITC and propidium iodide (PI). Plasma inflammation markers, IL‐6 and MCP‐1, were measured. Also, senescence was evaluated by acridine orange protocol. Lipid profile and aortic lipid deposition were also analyzed. Data are mean±SEM. Statistical analysis was performed with two way ANOVA test and Tukey pos hoc.Results: Aging and atherosclerosis increased ROS levels and apoptosis in HSC. Senescence was also increased with aging and atherosclerosis, especially in 24‐month‐old mice. Atherosclerotic adult and aged animals showed higher levels of IL‐6 and MCP‐1. As expected, atherosclerosis augmented total serum cholesterol levels and aortic lipid deposition.ConclusionThe present study provides evidence that aging and atherosclerosis can cause oxidant stress and impair HSC function. Such findings could lead to new insights into the mechanisms by which aging and atherosclerosis can affect immunity and inflammation.