Aging Cell Culture

Phipps, Sharla M. O.; Berletch, Joel B.; Andrews, Lori B.; Tollefsbol, Trygve O.

doi:10.1007/978-1-59745-361-5_2

Cited by 51 publications

(32 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…5,[28][29][30] Indeed, cultured fibroblast cell lines such as WI-38 have been used as models for aging in a number of studies. 31 However, all these studies used standard culture media, such as EMEM and DMEM. Our observations suggest that the aberrant methylation patterns observed in such studies were induced by high levels of folic acid in the culture media.…”

Section: Methodsmentioning

confidence: 99%

Supra-physiological folic acid concentrations induce aberrant DNA methylation in normal human cells in vitro

2012

View full text Add to dashboard Cite

Section: Methodsmentioning

confidence: 99%

Supra-physiological folic acid concentrations induce aberrant DNA methylation in normal human cells in vitro

2012

View full text Add to dashboard Cite

“…Thus, small cell mutants age slowly and are long-lived while large cells grow, divide and age dramatically faster in comparison. Specifically, intracellular RNA and protein contents increase with age, even though the synthesis of macromolecules decreases, and these elevations contribute to the increase in the cell size, numbers of inclusion bodies, and other cellular components [5]. …”

Section: Stem Cells and Cell Sizementioning

confidence: 99%

Cancer stem cells and cell size: A causal link?

Rycaj

Chen

et al. 2015

Seminars in Cancer Biology

View full text Add to dashboard Cite

The majority of normal animal cells are 10–20 µm in diameter. Many signaling mechanisms, notably PI3K/Akt/mTOR, Myc, and Hippo pathways, tightly control and coordinate cell growth, cell size, cell division, and cell number during homeostasis. These regulatory mechanisms are frequently deregulated during tumorigenesis resulting in wide variations in cell sizes and increased proliferation in cancer cells. Here, we first review the evidence that primitive stem cells in adult tissues are quiescent and generally smaller than their differentiated progeny, suggesting a correlation between small cell sizes with the stemness. Conversely, increased cell size positively correlates with differentiation phenotypes. We then discuss cancer stem cells (CSCs) and present some evidence that correlates cell sizes with CSC activity. Overall, the causal link between CSCs and cell size has been under-studied and remains to be rigorously assessed. In the future, optimizing methods for isolating cells based on size should help elucidate the connection between cancer cell size and CSC characteristics.

show abstract

“…Replicative senescence was used as an in vitro surrogate for aging [ 15 ]. With each replication, telomeres are shortened and cells eventually enter a stage of an irreversible proliferation arrest, termed replicative senescence [ 16 , 17 ]. The aim of present study is to evaluate the effects of mTOR inhibition on preserving the proliferative potential, enhancing anti-inflammatory reaction and alleviating oxidative stresses on human gingival fibroblasts (hGFs).…”

Section: Introductionmentioning

confidence: 99%

mTOR Inhibition Rejuvenates the Aging Gingival Fibroblasts through Alleviating Oxidative Stress

Xia

Sun

Xie

et al. 2017

Oxidative Medicine and Cellular Longevity

View full text Add to dashboard Cite

The aging periodontium may be vulnerable to periodontal pathogens and poor response to inflammation and susceptible to tumorigenesis. Human gingival fibroblasts (hGFs) through continuously replicative culture served as an in vitro surrogate for aging. To investigate the effects of the mechanistic target of rapamycin (mTOR) inhibition on the aging gingiva, we stimulated the high-passage hGFs with rapamycin (20 nmol/L) for 3 days and 30 days. The cellular and biological changes were examined by immunofluorescence, real-time PCR, ELISA, Western blotting, and flow cytometry. The data demonstrated that the inhibition of mTOR signaling led to fewer senescence-associated beta-galactosidase- (SA-β-Gal-) positive cells, delayed the onset of senescence, preserved the capability of proliferation, and lowered the expression levels of relevant senescence-associated markers, such as p16INK4a, p21CIP1a, interleukin-6 (IL-6), and IL-8. In addition, when infected by prominent periodontal pathogens, Porphyromonas gingivalis (ATCC 33277), rapamycin-pretreated groups decreased the expression of inflammatory cytokines (IL-6 and IL-8) compared with the control group. mTOR inhibition upregulated the gene expression of antioxidant components (Cat, Sod2, and Prdx3; P < 0.05) and consequently neutralized the excessive reactive oxygen species (ROS). In conclusion, our results indicated that mTOR inhibition might rejuvenate the aging gingiva to some extent and relieve inflammation through eliminating oxidative stress.

show abstract

Aging Cell Culture

Cited by 51 publications

References 26 publications

Supra-physiological folic acid concentrations induce aberrant DNA methylation in normal human cells in vitro

Supra-physiological folic acid concentrations induce aberrant DNA methylation in normal human cells in vitro

Cancer stem cells and cell size: A causal link?

mTOR Inhibition Rejuvenates the Aging Gingival Fibroblasts through Alleviating Oxidative Stress

Contact Info

Product

Resources

About