Aging Changes in the Alignment of Chromosomes after Human Chorionic Gonadotropin Stimulation May Be a Possible Cause of Decreased Fertility in Mice

Saito, Hidekazu; Koike, Kazuo; Saito, Toki; Nohara, Makoto; Kawagoe, Shinnosuke; Hiroi, Masahiko

doi:10.1159/000182754

Cited by 24 publications

(20 citation statements)

References 7 publications

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“…Our finding that in vitro culture reduces the incidence of spontaneous egg activation, which would negatively impact normal fertilization and development [13,43], suggests that mature MII eggs should be removed and placed into an appropriate culture medium soon after the completion of meiotic maturation or ovulation. This would avoid adverse effects of intrafollicular aging [44] or tubal spontaneous activation.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Culture Retards Spontaneous Activation of Cell Cycle Progression and Cortical Granule Exocytosis That Normally Occur in In Vivo UnfertilizedMouse Eggs1

Abbott

Kopf

et al. 1998

Biology of Reproduction

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We have previously demonstrated that metaphase II-arrested eggs recovered from oviducts at increasing times after hCG administration display a time-dependent spontaneous entry into anaphase, as well as release of cortical granules (CGs) and the associated modifications of the zona pellucida (ZP), a decrease in histone H1 and mitogen-activated protein kinase activities, and the recruitment of maternal mRNAs [Xu et al., Biol Reprod 1997; 57:743-750). These changes are correlated with the time-dependent increase in susceptibility of these eggs to undergo parthenogenetic activation. We report here the effect of culture of ovulated eggs, retrieved 13 or 16 h post-hCG administration and cultured in vitro for various periods of time, on the aforementioned parameters of egg activation and cell cycle resumption. In contrast to extended residence of the eggs in the oviduct, culture in vitro retarded cell cycle events associated with completion of the second meiotic reduction and inhibited CG release and the associated modifications of the ZP, as well as the recruitment of maternal mRNAs. The retardation or inhibition of these changes during in vitro culture resulted in eggs that were less susceptible to parthenogenetic activation than eggs that resided in the oviduct for comparable time periods. Results of these experiments indicate that egg culture in vitro (which likely occurs under suboptimal conditions) inhibits, rather than accelerates, the progression into the interphase-like state as compared to that seen in eggs residing in the oviduct for increasing periods of time. These results also suggest that, for studies focused on in vitro fertilization or egg activation, the ovulated eggs should be placed under appropriate in vitro conditions as soon as possible.

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Section: Discussionmentioning

confidence: 99%

In Vitro Culture Retards Spontaneous Activation of Cell Cycle Progression and Cortical Granule Exocytosis That Normally Occur in In Vivo UnfertilizedMouse Eggs1

Abbott

Kopf

et al. 1998

Biology of Reproduction

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“…Oocyte aging induces biochemical and molecular changes, such as the accumulation of reactive oxygen species (ROS), epigenetic modifications, and reduced expression of maturation‐related factor (Kikuchi et al, ). In addition, this process causes functional and structural defects, including exocytosis of cortical granules (Diaz & Esponda, a), hardening of the zona pellucida (Diaz & Esponda, b), chromosome and spindle anomalies (Saito et al, ), and mitochondrial dysfunction (Igarashi et al, ). These changes contribute to aging‐related defects in fertility and embryonic development.…”

Section: Introductionmentioning

confidence: 99%

Allicin protects porcine oocytes against damage during aging in vitro

Park

Lee

Yoon

et al. 2019

Molecular Reproduction Devel

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Allicin, a chemical component of garlic, has strong antioxidant activity and is thought to exert antiaging effects in vitro. We investigated whether allicin treatment would protect porcine oocytes and embryos from postovulatory aging mediated by apoptosis and autophagy. The rates of oocyte survival and polar body extrusion in samples treated with 1 µM allicin (1 AL) were significantly higher than in untreated samples (0 AL). In addition, 1 AL prevented defects in spindle formation and chromosome alignment, as well as decreases in the expression of maturation markers, during in vitro aging. In this study, we considered allicin to be a regulator of autophagy rather than an antioxidant or antiapoptotic agent. At the embryo level, although the cleavage rate after parthenogenetic activation was similar in all groups, the blastocyst formation rate was higher in the 1 AL group than in the 0 AL group. Our findings demonstrate that allicin effectively prevents the deterioration of porcine oocytes during aging in vitro, and could therefore be used to improve the quality of aged oocytes used in in vitro experiments.

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“…The changes include cortical granule exocytosis [1], zona pellucida (ZP) hardening [2], chromosome and spindle anomalies [3], decreased fertilization rates [4] and abnormal and/or retarded development of embryos/fetuses [5]. Therefore, it is important to determine the mechanisms that are implicated in oocyte aging, in order to develop strategies to delay oocyte aging and increase the time that may be needed to manipulate oocytes to perform assisted reproductive technologies (ARTs).…”

Section: Introductionmentioning

confidence: 99%

Melatonin prevents postovulatory oocyte aging and promotes subsequent embryonic development in the pig

Wang

Gao

Chen

et al. 2017

Aging

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Oxidative stress is known as a major contributing factor involved in oocyte aging, which negatively affects oocyte quality and development after fertilization. Melatonin is an effective free radical scavenger and its metabolites AFMK and AMK are powerful detoxifiers that eliminate free radicals. In this study, we used porcine oocytes to test the hypothesis that melatonin could scavenge free radicals produced during oocyte aging, thereby maintaining oocyte quality. We compared reactive oxygen species levels, apoptosis levels, mitochondrial membrane potential ratios, total glutathione contents and expression levels in fresh, aged and melatonin-treated aged porcine oocytes and observed the percentage of blastocyst formation following parthenogenetic activation. We found that melatonin could effectively maintain the morphology of oocytes observed in control oocytes, alleviate oxidative stress, markedly decrease early apoptosis levels, retard the decline of mitochondrial membrane potential and significantly promote subsequent embryonic development in oocytes aged for 24 hr in vitro. These results strongly suggest that melatonin can prevent postovulatory oocyte aging and promote subsequent embryonic development in the pig, which might find practical applications to control oocyte aging in other mammalian species including humans to maintain the quality of human oocytes when performing clinical assisted reproductive technology.

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Aging Changes in the Alignment of Chromosomes after Human Chorionic Gonadotropin Stimulation May Be a Possible Cause of Decreased Fertility in Mice

Cited by 24 publications

References 7 publications

In Vitro Culture Retards Spontaneous Activation of Cell Cycle Progression and Cortical Granule Exocytosis That Normally Occur in In Vivo UnfertilizedMouse Eggs1

In Vitro Culture Retards Spontaneous Activation of Cell Cycle Progression and Cortical Granule Exocytosis That Normally Occur in In Vivo UnfertilizedMouse Eggs1

Allicin protects porcine oocytes against damage during aging in vitro

Melatonin prevents postovulatory oocyte aging and promotes subsequent embryonic development in the pig

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