Aging of porous silicon in physiological conditions: Cell adhesion modes on scaled 1D micropatterns

Noval, Álvaro Muñoz; Vaquero, Vanessa Sánchez; Quijorna, Esther Punzón; Costa, Vicente Torres; Pérez, Darío Gallach; Méndez, Laura González; Montero, I.; Martín-Palma, Raúl J.; Font, Aurelio Climent; Ruiz, Josefa Predestinación García; Manso‐Silván, Miguel

doi:10.1002/jbm.a.34108

Cited by 24 publications

(13 citation statements)

References 35 publications

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“…These spectra indicate that photoluminescence intensity in the blue range increases progressively upon exposure to PBS up to 1,000 min (i.e., up to typical cell culture periods) as illustrated in Figure 3 in agreement with previously described aging in alternative conditions [29]. This indicates increased oxidation of the PS with a high density of silanol Si-OH groups, which may be responsible of a readily observed increased hydrophilicity [30,31]. …”

Section: Resultssupporting

confidence: 86%

Nanostructured porous silicon micropatterns as a tool for substrate-conditioned cell research

et al. 2012

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The localized irradiation of Si allows a precise patterning at the microscale of nanostructured materials such as porous silicon (PS). PS patterns with precisely defined geometries can be fabricated using ion stopping masks. The nanoscale textured micropatterns were used to explore their influence as microenvironments for human mesenchymal stem cells (hMSCs). In fact, the change of photoluminescence emission from PS upon aging in physiological solution suggests the intense formation of silanol surface groups, which may play a relevant role in ulterior cell adhesion. The experimental results show that hMSCs are sensitive to the surface micropatterns. In this regard, preliminary β-catenin labeling studies reveal the formation of cell to cell interaction structures, while microtubule orientation is strongly influenced by the selective adhesion conditions. Relevantly, Ki-67 assays support a proliferative state of hMSCs on such nanostructured micropatterns comparable to that of standard cell culture platforms, which reinforce the candidature of porous silicon micropatterns to become a conditioning structure for in vitro culture of HMSCs.

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Section: Resultssupporting

confidence: 86%

Nanostructured porous silicon micropatterns as a tool for substrate-conditioned cell research

et al. 2012

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“…Furthermore, surface micro-and nano-patterning is becoming an important means for enhancing the performance of materials such as creating superhydrophobic surfaces with hierarchical meshporous structures, 14 reprogramming the cell shape, 15 or enlarging cell culture harvest. 16 For the particular case of nanoPS, patterns have been used to promote cell binding or growth [9][10][11]17,18 or to produce label-free biosensors, 3,19 the detection mechanism being based on changes either in the photoluminescence spectra or in the diffraction pattern.…”

Section: Introductionmentioning

confidence: 99%

Ultraviolet laser patterning of porous silicon

Vega¹,

Peláez

Kuhn

et al. 2014

Journal of Applied Physics

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The role of asymmetric excitation in self-organized nanostructure formation upon femtosecond laser ablation AIP Conf. Proc. 1464, 428 (2012) . In addition, the percentage of transformed to non-transformed region normalized to the pattern period follows similar fluence dependence regardless the period and thus becomes an excellent control parameter. This dependence is fitted within a thermal model that allows for predicting the in-depth profile of the pattern. The model assumes that transformation occurs whenever the laser-induced temperature increase reaches the melting temperature of nanoPS that has been found to be 0.7 of that of crystalline silicon for a porosity of around 79%. The role of thermal gradients across the pattern is discussed in the light of the experimental results and the calculated temperature profiles, and shows that the contribution of lateral thermal flow to melting is not significant for pattern periods !6.3 lm.

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“…The layers were assumed to be a mixture of Si and air (% porosity). A good agreement was achieved for a first thin layer (6-8 nm) of low porosity (57%) on top of the c-Si substrate followed by an intermediate thin layer (19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29) nm) of 67%-70% porosity and a top thick layer (293-335 nm) having 81% of porosity. Thus, the total thicknesses is 320/370 nm, where the first and second number, respectively, correspond to the 7.5/10 s etching time.…”

Section: Preparation Of Nanopsmentioning

confidence: 69%

“…In this case, the cells show extended podia and decentralized nucleus suggesting an active polarization. 20…”

Section: Resultsmentioning

confidence: 99%

“…HMSCs were directly cultured with no previous functionalization of the nanoPS substrates, on some of the patterns following a procedure similar to that reported elsewhere. 20 Human bone marrow samples (2-4 ml) from healthy donors were provided by Hospital Universitario La Princesa (Madrid, Spain). Cells were collected by centrifugation on 70% Percoll gradient and seeded at 200,000/cm 2 in Dulbecco's modified Eagle's medium with low glucose (DMEM-LG) supplemented with 10% fetal bovine serum (FBS).…”

Section: Characterization and Cell Culturementioning

confidence: 99%

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Laser fabrication of porous silicon-based platforms for cell culturing

Peláez

Afonso

Vega³

et al. 2013

J. Biomed. Mater. Res.

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In this study, we explore the selective culturing of human mesenchymal stem cells (hMSCs) on Si-based diffractive platforms. We demonstrate a single-step and flexible method for producing platforms on nanostructured porous silicon (nanoPS) based on the use of single pulses of an excimer laser to expose phase masks. The resulting patterns are typically 1D patterns formed by fringes or 2D patterns formed by circles. They are formed by alternate regions of almost unmodified nanoPS and regions where the nanoPS surface has melted and transformed into Si nanoparticles. The patterns are produced in relatively large areas (a few square millimeters) and can have a wide range of periodicities and aspect ratios. Direct binding, that is, with no previous functionalization of the pattern, alignment, and active polarization of hMSCs are explored. The results show the preferential direct binding of the hMSCs along the transformed regions whenever their width compares with the dimensions of the cells and they escape from patterns for smaller widths suggesting that the selectivity can be tailored through the pattern period. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2013.

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Aging of porous silicon in physiological conditions: Cell adhesion modes on scaled 1D micropatterns

Cited by 24 publications

References 35 publications

Nanostructured porous silicon micropatterns as a tool for substrate-conditioned cell research

Nanostructured porous silicon micropatterns as a tool for substrate-conditioned cell research

Ultraviolet laser patterning of porous silicon

Laser fabrication of porous silicon-based platforms for cell culturing

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