Aging of Skeletal Muscle Does Not Affect the Response of Satellite Cells to Denervation

Dedkov, Eduard I.; Borisov, Andrei B.; Wernig, A.; Carlson, Bruce M.

doi:10.1177/002215540305100701

Cited by 45 publications

(47 citation statements)

References 45 publications

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“…Similar age-related declines have also been documented for epidermal stem cells (Michel et al, 1996) and skeletal muscle satellite cells (Snow, 1977;Gibson and Schultz, 1983;Dedkov et al, 2003). At least three factors could contribute to the difference between these systems and the hematopoetic system.…”

Section: Discussionsupporting

confidence: 66%

Neural Stem Cell Detection, Characterization, and Age-Related Changes in the Subventricular Zone of Mice

Maslov¹,

Barone²,

Plunkett³

et al. 2004

J. Neurosci.

479

422

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The mammalian brain contains neural stem cells (NSCs) that allow continued neurogenesis throughout the life of the animal. However, neurogenesis is known to decline during aging and, to the extent that neurogenesis is required for normal CNS function, this may contribute to neurodegenerative disease. Decreased neurogenesis could result from loss of NSCs or dysfunction at some later step, and distinguishing these possibilities is important for understanding the cause of the decline. However, because of the inability to distinguish NSCs from their rapidly dividing progeny in situ, it has not been possible to quantitatively assess the NSC populations in young and old animals. In this report we show that the G1 phase-specific expression of the replication factor Mcm2 is a useful marker for detecting slowly cycling putative NSCs in situ and confirm the identity of these cells using both cytosine ␤-D-arabinofuranoside (Ara-C) treatment and a double nucleoside analog-labeling technique. The ability to distinguish NSCs from proliferative progenitors has allowed characterization of the expression of several markers including Nestin, Musashi, and GFAP in these different cell types. Furthermore, comparison of the NSC populations in the subventricular zones of young (2-4 months) and old (24 -26 months) mice demonstrates an approximately twofold reduction in the older mice. A similar twofold reduction is also observed in the number of neurospheres recovered in culture from old relative to young animals. The reduction in the neural stem cell population documented here is sufficient to account for the reduced level of neurogenesis in old animals.

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Section: Discussionsupporting

confidence: 66%

Neural Stem Cell Detection, Characterization, and Age-Related Changes in the Subventricular Zone of Mice

Maslov¹,

Barone²,

Plunkett³

et al. 2004

J. Neurosci.

479

422

View full text Add to dashboard Cite

show abstract

“…8a-c), which confirmed that some of these cells were indeed activated myoblasts localized between muscle fibers in the endomysial space. It has been shown earlier that activated satellite cells intensely express NCAM, and this protein is considered one of their markers (Irintchev et al 1994;Dedkov et al 2003).…”

Section: Resultsmentioning

confidence: 99%

Abortive myogenesis in denervated skeletal muscle: differentiative properties of satellite cells, their migration, and block of terminal differentiation

2005

Self Cite

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Little is known about the biological properties of myogenic satellite cells during post-denervation muscle atrophy. The present study investigated the differentiative capacity of satellite cells and their involvement in the compensatory regenerative process in long-term denervated rat muscle. Electron microscopy and immunocytochemical labeling of muscle tissue 1-18 months following denervation demonstrated that despite activation of satellite cells, myogenesis in denervated muscle is abortive and does not lead to the formation of normal muscle fibers. Small sizes, poor development of the contractile system in newly formed denervated myotubes, and the absence of satellite cells on the surface indicate that their differentiation typically does not progress to terminal stages. Many immature myotubes degenerate, and others survive but are embedded in a collagen lattice near their parent fibers. Interestingly, newly formed myotubes located on the surface of parent muscle fibers beneath the basal lamina typically did not contain developed myofibrils. This suggests that the contacts of daughter and parent muscle fibers block myofibrillogenesis. Assembly of sarcomeres in most cases occurs following complete spatial separation of daughter and parent muscle fibers. Another manifestation of the involvement of myogenic precursors in abortive myogenesis is the formation of clusters of underdeveloped branching myotubes surrounded by a common basal lamina. We found that myoblasts can also fuse directly with differentiated muscle fibers. The presence of satellite cells near the openings in the basal lamina and in the interstitial space indicates that myogenic precursors can migrate through the basal lamina and form myotubes at a distance from parent fibers. Our data may explain why long-term denervated skeletal muscle has a poor capacity for regeneration and functional restoration.

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“…A more recent study by Shavlakadze et al (2010) similarly showed a delay in the onset of regeneration after autografting muscle into a geriatric mouse host, though again the long-term formation of new muscle was unaffected and there was no effect of the geriatric host on regenerating muscle. Other animal studies which have used a variety of different models including chronic lowfrequency electrical stimulation (Putman et al 2001), denervation (Dedkov et al 2003) as well cross-transplantation in mdx mice (Boldrin et al 2009) have similarly observed no age related impairment to muscle repair. Taking the above into account, it is also possible to speculate that there is a slower activation of young cells cultured in old serum that might be detectable before day three of culture, but the data presented show that even if this were the case, these initial differences have no effect on later myogenic responses.…”

Section: Accepted M Manuscriptmentioning

confidence: 97%

Sera from young and older humans equally sustain proliferation and differentiation of human myoblasts

George

Velloso

Alsharidah

et al. 2010

Experimental Gerontology

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Please cite this article as: George, Tomasz, Velloso, Cristiana P., Alsharidah, Mansour, Lazarus, Norman R., Harridge, Stephen D.R., Sera from young and older humans equally sustain proliferation and differentiation of human myoblasts, Experimental Gerontology (2010Gerontology ( ), doi: 10.1016Gerontology ( /j.exger.2010 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Running title: Ageing serum and human satellite cell behaviour A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT AbstractUsing a human primary muscle cell culture model the behavior of myoblasts (satellite cells) cultured in human serum obtained from either young or elderly individuals was studied. Serum was obtained from a total of 13 young (7 male and 6 females aged, 23-36 years) and 9 elderly (4 male and 5 females aged 69-84 years) subjects and used in a number of experiments. Myoblasts were extracted from human muscle biopsy samples taken from the vastus lateralis. In the first experiment myoblasts were isolated immediately after extraction from the biopsy in media containing human sera to examine its effects on the onset and progression of Ki67 and desmin expression. No effect of the age of the serum was observed at 3, 5 or 7 days of proliferation. In addition, cells that had been expanded initially in optimum myoblast growth medium (GM, containing foetal calf serum and additional growth factors) prior to culture in medium containing 15% human serum were studied. The proportion of proliferating muscle cells co expressing desmin and Ki67 antigens after 46 hours was again similar in the young and old serum conditions. Culturing these myoblasts in media containing 2% human serum to study their fusion and differentiation also resulted in no difference between young and old serum conditions in terms of the percentage of nuclei inside myosin heavy chain positive myotubes. Despite the variability of different samples of myoblasts, the age of the serum has no affect on the expression of any measured index.

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Aging of Skeletal Muscle Does Not Affect the Response of Satellite Cells to Denervation

Cited by 45 publications

References 45 publications

Neural Stem Cell Detection, Characterization, and Age-Related Changes in the Subventricular Zone of Mice

Neural Stem Cell Detection, Characterization, and Age-Related Changes in the Subventricular Zone of Mice

Abortive myogenesis in denervated skeletal muscle: differentiative properties of satellite cells, their migration, and block of terminal differentiation

Sera from young and older humans equally sustain proliferation and differentiation of human myoblasts

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