Agmatine protects cultured retinal ganglion cells from tumor necrosis factor-alpha-induced apoptosis

Hong, Samin; Kim, Chan Yun; Lee, Jong Eun; Seong, Gong Je

doi:10.1016/j.lfs.2008.10.006

Cited by 65 publications

(35 citation statements)

References 40 publications

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“…The red formazan product was measured at 490 nm using an ELISA plate reader (Vmax; Molecular Device, USA). Cytotoxicity was calculated as the proportion of injured cells to the total cell population (14). Data are reported as means ± SEM.…”

Section: Cytotoxicity Assaymentioning

confidence: 99%

Protective mechanism of agmatine pretreatment on RGC-5 cells injured by oxidative stress

Ikoma

Hong

Kim

et al. 2010

Braz J Med Biol Res

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Section: Cytotoxicity Assaymentioning

confidence: 99%

Protective mechanism of agmatine pretreatment on RGC-5 cells injured by oxidative stress

Ikoma

Hong

Kim

et al. 2010

Braz J Med Biol Res

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“…Travmatik hasar modeli kullanılarak yapılan bir çalışmada histopatolojik olarak Agm'in beyin hasarını azalttığı benzer bir çalışmada hipoksiye bağlı TNF-α salımını inhibe ederek retinal hücreleri koruduğu gösteril-miştir (36,37). Retinal gangliyon hücrelerini TNF-α'ya bağlı apoptozisi baskılayarak engellediği gösterilmiştir (38,39).…”

Section: Discussionunclassified

Effects of Agmatine in Streptozotocine induced Experimental Alzheimer Model

Yalabık¹,

Şehirli²,

Utkan³

et al. 2013

MUSBED

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Streptozotosin ile oluşturulan deneysel alzheimer modelinde agmatinin etkilerinin araştırılması Amaç: Alzheimer geri dönüşümsüz ve ilerleyici hafıza kaybını takiben tam demansla karakterize nörodejenaratif bir bozukluktur. Yaşlanmayla insanlarda en sık görülen demans formudur. Bu çalışmada sıçanlarda intraserebroventriküler (i.c.v) streptozotosin (STZ) ile oluşturulmuş Alzheimer modelinde agmatinin (Agm) öğrenme ve bellek fonksiyonlarına etkisi ve bu etkinin oksidatif hasar açısından mekanizması araştırılmıştır. Gereç ve Yöntem: Çalışmamızda Sprague-Dawley erkek sıçanlar kullanılmış, kontrol, sham opere, STZ ve STZ+Agm olmak üzere 4 gruba ayrılmışlardır. Alzheimer modeli oluşturmak için stereotaksik yöntem ile sıçanlara i.c.v olarak bilateral STZ 3 mg/kg dozda 48 saat arayla 2 defa uygulanmış ve ilk uygulamayı takiben 14 gün beklenmiştir. On beşinci günde pasif sakınma tesi ile öğrenme ve bellek fonksiyonları değerlendirilmiştir. Öğrenmesinin bozulduğu gösterilen sıçanlara 7 gün boyunca günde 2 defa 40mg/kg dozda Agm intraperitoneal (i.p.) olarak uygulanmış ve takiben aynı testler tekrarlanmıştır. Tüm gruplar davranış testleri bittikten sonra (21. günde) dekapite edilerek beyin dokuları toplanmış ve oksidatif hasarı değerlendirmek için malondialdehit (MDA), glutatyon (GSH) seviyeleri ve myeloperoksidaz (MPO) aktivitesi ölçümleri yapılmıştır. Bulgular: İntraserebroventriküler STZ ile oluşturulmuş alzheimer modelinde kognitif fonksiyonlarında belirgin bozulma olduğu ve bu bozulmaya oksidatif hasarın eşlik ettiği gösterilmiştir. Agmatin tedavisi ile hem kognitif fonksiyon hem de oksidatif hasarı önlemiştir. Sonuç: Bu bulgulara dayanarak endojen bir madde olan agmatinin alheimer etyopatogenezinde önemli bir düzenleyici olabileceği düşünülmüştür. Anahtar sözcükler: Agmatin, Alzheimer, intraserebroventiküler streptozotosin, oksidatif hasar, öğrenme ve bellek ABS TRACT Effects of agmatine in streptozotocine induced experimental alzheimer modelObjectives: Alzheimer's disease is a neurodegenerative disorder characterized by progressive loss of memory followed by complete dementia. Furthermore it is the commonest form of dementia affecting older people. In this study we investigated the role of agmatine (Agm) on learning and memory and on parameters of oxidative stress in intracerebroventricular (i.c.v) streptozotocin (STZ) model of Alzheimer's disease in rats. Materials and Methods: We grouped rats as: naive control, sham operated, STZ and STZ+agmatine. Rats were injected bilaterally i.c.v STZ (3mg/kg) using stereotaxic frame on day 1 and 3 to induce experimental Alzheimer's disease. After 14 days from 1 st STZ injection, learning and memory impairment of rats were observed in passive avoidance. Following this test, agmatine administered intraperitoneal (i.p.) 40mg/kg twice daily for 7 days. These animals were again subjected to these behavioral tests after 7 days agmatine treatment. On the 21 st day after 1 st STZ injection all groups were sacrificed by decapitation and brain tissues were collected for oxidative stress m...

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“…Apoptotic or necrotic cell death caused by BSO plus glutamate was characterized by double staining the cells with Hoechst 33342 and PI (Hong et al 2009). Cells were stained with 8 μM Hoechst 33342 and 1.5 μM PI for 30 min at 37°C.…”

Section: Microscopic Analysis For Cell Viability By Pi and Hoechst 33mentioning

confidence: 99%

Edible Seaweed, Eisenia bicyclis, Protects Retinal Ganglion Cells Death Caused by Oxidative Stress

Kim

Kang

et al. 2012

Mar Biotechnol

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The purpose of the present study was to determine whether edible seaweed, Eisenia bicyclis, is effective in blunting the negative influence of N-methyl-D-aspartate (NMDA) on rat retinas and of oxidative stress-induced transformed retinal ganglion cell (RGC-5 cell line) death. The ethanol extract of E. bicyclis (EEEB) significantly attenuated the negative insult of L: -buthionine-(S,R)-sulfoximine plus glutamate on RGC-5 cells. Treatment of the RGC-5 cells with EEEB reduced the reactive oxygen species and recovered the reduced glutathione level caused by various radical species such as H(2)O(2), OH·, or O(2)·(-). Moreover, EEEB inhibited lipid peroxidation on rat brain homogenates caused by sodium nitroprusside. Applying NMDA to the retina affected the thickness of the inner plexiform layer (IPL) and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) produced a positive effect on ganglion cells. Importantly, EEEB protected the thinning of IPL and increased TUNEL positive cells in the ganglion cell layer (GCL). Five phlorotannin derivatives were isolated using chromatographic methods and liquid chromatography-mass spectroscopy analysis which has been known as an antioxidant. In conclusion, EEEB has a neuroprotective effect in vitro and in vivo. Furthermore, the major constituents of this extract, phlorotannins, could possibly be active compounds due to their antioxidative potency.

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Agmatine protects cultured retinal ganglion cells from tumor necrosis factor-alpha-induced apoptosis

Cited by 65 publications

References 40 publications

Protective mechanism of agmatine pretreatment on RGC-5 cells injured by oxidative stress

Protective mechanism of agmatine pretreatment on RGC-5 cells injured by oxidative stress

Effects of Agmatine in Streptozotocine induced Experimental Alzheimer Model

Edible Seaweed, Eisenia bicyclis, Protects Retinal Ganglion Cells Death Caused by Oxidative Stress

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