Agonist-induced calcium response in single human platelets assayed in a microfluidic device

In this work we demonstrate a new microfluidic method for the rapid assessment of platelet size and morphology in whole blood. The device continuously fractionates particles according to size by displacing them perpendicularly to the fluid flow direction in a micro-fabricated post array. Whole blood, labeled with the fluorescent, platelet specific, antibody PE-anti-CD41, was run through the device and the positions of fluorescent objects noted as they exited the array. From this, histograms of platelet size were created which show marked increases in size after exposure to thrombin or a temperature of 4 degrees C. We infer that the well known morphological changes that occur during activation are causing the observed increase in size.

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“…Fig 6. Plot of mean platelet hydrodynamic size vs. temperature, including the thrombin activated sample.…”

mentioning

confidence: 99%

Microfluidic device for label-free measurement of platelet activation

et al. 2008

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“…By constructing a channel with a narrowing in the detection zone, individual responding platelets could be detected while passing through the channel (Figure 1G). This method demonstrated increases in intracellular Ca 2þ levels following stimulation by the PAR-1 agonist SFLLRN, or ADP [37]. An innovative microfluidic method to measure label-free platelet activation is based on the principle of deterministic lateral displacement to separate platelets on-chip from other cell types based on their size [38].…”

Section: Platelet Activation Under Flowmentioning

confidence: 99%

Monitoringin vitrothrombus formation with novel microfluidic devices

et al. 2012

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Cardiovascular disease is a major cause of mortality globally and is subject to ongoing research to improve clinical treatment. It is established that activation of platelets and coagulation are central to thrombosis, yet at different extents in the arterial and venous system. In vitro perfusion chamber technology has contributed significant knowledge on the function of platelets in the thrombotic process under shear conditions. Recent efforts to downscale this technique with a variety of microfluidic devices has opened new possibilities to study this process under precisely controlled flow conditions. Such microfluidic devices possess the capability to execute platelet function tests more quickly than current assays, while using small blood samples. Gradually becoming available to the clinic now, they may provide a new means to manage the treatment of cardiovascular diseases, although accurate validation studies still are missing. This review highlights the progress that has been made in monitoring aspects of thrombus formation using microfluidic devices.

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“…Tran et al, 2005, studied agonist-induced calcium response in single human platelets using a microfluidic device for identification of signaling pathways. Shen et al, 2008 identified the range of shear rates that limit initiation of coagulation pathways using a combination of surface areas treated with tissue factor protein and native platelets.…”

Section: Microfluidic Devicesmentioning

confidence: 99%

Microfluidic devices for modeling cell–cell and particle–cell interactions in the microvasculature

Prabhakarpandian

Shen

Pant

et al. 2011

Microvascular Research

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Cell-fluid and cell-cell interactions are critical components of many physiological and pathological conditions in the microvasculature. Similarly, particle-cell interactions play an important role in targeted delivery of therapeutics to tissue. Development of in vitro fluidic devices to mimic these microcirculatory processes has been a critical step forward in our understanding of the inflammatory process, development of nano-particulate drug carriers, and developing realistic in vitro models of the microvasculature and its surrounding tissue. However, widely used parallel plate flow based devices and assays have a number of important limitations for studying the physiological conditions in vivo. In addition, these devices are resource hungry and time consuming for performing various assays. Recently developed, more realistic, microfluidic based devices have been able to overcome many of these limitations. In this review, an overview of the fluidic devices and their use in studying the effects of shear forces on cell-cell and cell-particle interactions is presented. In addition, use of mathematical models and Computational Fluid Dynamics (CFD) based models for interpreting the complex flow patterns in the microvasculature are highlighted. Finally, the potential of 3D microfluidic devices and imaging for better representing in vivo conditions under which cell-cell and cell-particle interactions take place are discussed.

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Agonist-induced calcium response in single human platelets assayed in a microfluidic device

Cited by 20 publications

References 28 publications

Microfluidic device for label-free measurement of platelet activation

Microfluidic device for label-free measurement of platelet activation

Monitoringin vitrothrombus formation with novel microfluidic devices

Microfluidic devices for modeling cell–cell and particle–cell interactions in the microvasculature

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