Agricultural Coconut Cultivation Wastes as Feedstock for Lignocellulosic Ethanol Production by Kluyveromyces marxianus

Gomes, Márcia Andréa; Rocha, Martha Suzana Rodrigues dos Santos; Barbosa, Kledson Lopes; Abreu, Íthalo Barbosa Silva de; Pimentel, Wagner Roberto de Oliveira; Silva, Carlos Eduardo de Farias; Almeida, Renata Maria Rosas Garcia; Albuquerque, Elaine Christine de Magalhães Cabral; Vieira, Rosana Correia

doi:10.1007/s12649-021-01345-w

Cited by 6 publications

(1 citation statement)

References 39 publications

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“…However, approximately 80% of the net weight of the fruit is formed by the shell, which is generally discarded in the environment [3,4] and can cause a series of problems in the regions of extraction, processing, and commerce [5]. Alternatively, the residue can be used in bioprocesses, such as the production of enzymes [6,7] and cellulosic ethanol [3,[8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Extraction of Cellulases Produced through Solid-State Fermentation by Trichoderma reesei CCT-2768 Using Green Coconut Fibers Pretreated by Steam Explosion Combined with Alkali

Campos,

Asevedo,

Souza Filho

et al. 2024

Biomass

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The industrial processing of coconut to produce valuable foods, such as water and milk, generates large volumes of waste, especially the fruit shell. Despite this, material can be used in bioprocess applications, e.g., the production of enzymes, its recalcitrance hinders the cultivation of microorganisms, and low productivity is usually achieved. In this study, the production of cellulolytic enzymes through solid-state fermentation (SSF) and their extraction was investigated using the green coconut fiber pretreated by steam explosion, followed by alkali. The fungus Trichoderma reesei CCT-2768 was cultivated, using an experimental design, to study the effect of the water activity and the amount of biomass in the reactor. The combination of the pretreatment strategies yielded more porous biomass, with less hemicellulose (5.38%, compared to 10.15% of the raw biomass) and more cellulose (47.77% and 33.96% in the pretreated and raw biomasses, respectively). The water activity significantly affected the production of cellulases, with maximum activity yielded at the highest investigated value (0.995). Lastly, the extraction of the enzymes from the cultivation medium was studied, and a 9 g/L NaCl solution recovered the highest CMCase and FPase activities (5.19 and 1.19 U/g, respectively). This study provides an important contribution to the valorization of the coconut residue through (i) the application of the steam explosion technology to optimize the production of cellulases using the SSF technology and (ii) their extraction using different solvents.

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