Modern agriculture increasingly demands an alternative to synthetic chemicals (fertilizers and pesticides) in order to respond to the changes in international law and regulations, but also consumers’ needs for food without potentially toxic residues. Microbial (arbuscular mycorrhizal and plant growth promoting rhizobacteria: Azotobacter, Azospirillum and Rizhobium spp.) and non-microbial (humic substances, silicon, animal- and vegetal-based protein hydrolysate and macro- and micro-algal extracts) biostimulants represent a sustainable and effective alternative or complement for their synthetic counterparts, bringing benefits to the environment, biodiversity, human health and economy. The Special Issue “Toward a sustainable agriculture through plant biostimulants: from experimental data to practical applications” compiles 34 original research articles, 4 review papers and 1 brief report covering the implications of microbial and non-microbial biostimulants for improving seedling growth and crop performance, nutrient use efficiency and quality of the produce as well as enhancing the tolerance/resistance to a wide range of abiotic stresses in particular salinity, drought, nutrient deficiency and high temperature. The present compilation of high standard scientific papers on principles and practices of plant biostimulants will foster knowledge transfer among researchers, fertilizer and biostimulant industries, stakeholders, extension specialists and farmers, and it will enable a better understanding of the physiological and molecular mechanisms and application procedure of biostimulants in different cropping systems.