Agrimonia eupatoria protects against chronic ethanol-induced liver injury in rats

Yoon, Sungpil; Koh, Eun-Ji; Kim, Chang-Soo; Zee, Ok-Pyo; Kwak, Jong-Hwan; Jeong, Won‐Jin; Kim, Jeehyun; Lee, Sun-Mee

doi:10.1016/j.fct.2012.04.005

Cited by 52 publications

(34 citation statements)

References 38 publications

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“…Direct evidence has demonstrated that circulating endotoxin level was significantly increased in rodents and humans following long-term exposure to ethanol (Adachi, Moore, Bradford, Gao, & Thurman, 1995;Nanji, Khettry, & Sadrzadeh, 1994;Wang et al, 2011). After binding to lipopolysaccharide (LPS)-binding protein (LBP), gut-derived endotoxin is recognised by Toll-like receptor-4 (TLR4) and its coreceptors, such as CD14, on the cell membrane of Kupffer cells in liver, activating the Kupffer cells and initiating the TLR4-mediated nuclear factor κB (NF-κB) signalling (Hritz et al, 2008;Yoon et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Saponins isolated from the leaves of Panax notoginseng protect against alcoholic liver injury via inhibiting ethanol-induced oxidative stress and gut-derived endotoxin-mediated inflammation

Wang

Zhang

Liu

et al. 2015

Journal of Functional Foods

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Available online A B S T R A C TEffects of saponins from leaves of Panax notoginseng (LPNS) against alcoholic liver injury were evaluated, and their probable molecular mechanisms were further elucidated in a mouse model of chronic-plus-single-binge ethanol feeding. Alcohol exposure dramatically increased plasma levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), triacylglycerol (TG) and endotoxin; these elevations were attenuated by treatment with LPNS at a dose of 100 or 300 mg/kg. LPNS was also found to suppress thiobarbituric acid reactive substances (TBARS) production, enhance glutathione (GSH) level and superoxide dismutase (SOD) activity in liver. The protective of LPNS against oxidative stress was associated with down-regulation of hepatic cytochrome P450 2E1 (CYP2E1), and upregulation of nuclear factor erythroid 2-related factor 2 (Nrf2) and haem oxygenase-1 (HO-1). Additionally, LPNS ameliorated gut leakiness via upregulating the expressions of tight junction proteins, and suppressed endotoxin-mediated inflammation via down-regulating toll-like receptor-4 (TLR-4), CD14 and p-NF-κB p65. These results demonstrate that treatment with LPNS effectively protects against alcoholic liver injury through reducing ethanol-induced oxidative stress and ameliorating gut-derived endotoxin-mediated inflammation. Alcoholic liver injurySaponins from the leaves of Panax notoginseng Oxidative stress Gut-derived endotoxin Inflammation Abbreviations: ALD, alcoholic liver disease; ALT, alanine aminotransferase; AST, aspartate aminotransferase; BW, body weight; CYP2E1, cytochrome P450 2E1; GSH, glutathione; H2O2, hydrogen peroxide; HO-1, haem oxygenase-1; HPLC, high performance liquid chromatography; HRP, horseradish peroxidase; IL-6, interleukin 6; LBP, lipopolysaccharide-binding protein; LPNS, saponins from leaves of Panax

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Section: Introductionmentioning

confidence: 99%

Saponins isolated from the leaves of Panax notoginseng protect against alcoholic liver injury via inhibiting ethanol-induced oxidative stress and gut-derived endotoxin-mediated inflammation

Wang

Zhang

Liu

et al. 2015

Journal of Functional Foods

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“…25) In brief, whole protein was extracted from liver tissue and analyzed with a bicinchoninic acid (BCA) protein concentration assay kit (Shanghai Haoran Bio Technologies Co., Ltd., China). A total of 20 µg of whole protein was used to determine the content of Toll-like receptor 4 (TLR4), CD14, myeloid differentiation factor 88 (MyD88), TIR domain-containing adaptor-inducing interferon-b (TRIF), cyclooxygenase 2 (COX-2), and inducible nitric oxide synthase (iNOS).…”

Section: Fig 3 the Effects Of Aapc On Hepatic Alcohol Metabolizing mentioning

confidence: 99%

Retraction: Asiatic Acid from <i>Potentilla chinensis</i> Attenuate Ethanol-Induced Hepatic Injury <i>via</i> Suppression of Oxidative Stress and Kupffer Cell Activation

Wei

Huang

et al. 2013

Biological & Pharmaceutical Bulletin

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This study examined the effect of Asiatic acid from Potentilla chinensis (AAPC) on chronic ethanolinduced hepatic injury. Rats underwent intragastric administration of ethanol (5.0-9.0 g/kg) once a day for 12 weeks. A subset of rats were also intragastrically treated with AAPC (2, 4 or 8 mg/kg) once a day. In the end, AAPC treatment significantly protected against ethanol-induced liver injury, as evidenced by the decrease in serum alanine and aspartate aminotransferases levels and the attenuation of histopathological changes in rats. Additionally, AAPC significantly decreased blood alcohol and acetaldehyde concentrations by enhancing alcohol dehydrogenase and aldehyde dehydrogenase activities. Mechanistically, studies showed that AAPC remarkably alleviated the formations of malondialdehyde and myeloperoxidase, restored impaired antioxidants, including superoxide dismutase, glutathione peroxidase, glutathione reductase and catalase, and inhibited cytochrome P450 (CYP)2E1 activity. Moreover, the over-expression of cytokines, such as tumor necrosis factor (TNF)-α, interleukin (IL)-1β, inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), the elevated plasma endotoxin level and the up-regulated Toll-like receptor 4 (TLR4), CD14 and myeloid differentiation factor 88 (MyD88) as well as nuclear factor-κB were also suppressed by AAPC in ethanol-intoxicated rats. In conclusion, the protective effect of AAPC on ethanol-induced hepatotoxicity was mainly due to its ability to attenuate oxidative stress and inhibit Kupffer cell activation by decreasing the level of plasma endotoxin and the expression of TLR4, CD14 and MyD88.

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“…To study liver damage caused by alcohol, different experimental models have been developed, such as the Lieber-DeCarli liquid diet model [30,31], and the chronic binge model [32,33]. Pair-feeding is adopted in the Lieber-DeCarli liquid diet model, which eliminates influence from factors other than ethanol.…”

Section: Resultsmentioning

confidence: 99%

The Effects of Syzygium samarangense, Passiflora edulis and Solanum muricatum on Alcohol-Induced Liver Injury

Zhang

Zhou

Wang

et al. 2016

IJMS

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Previous studies have shown that fruits have different effects on alcohol metabolism and alcohol-induced liver injury. The present work selected three fruits and aimed at studying the effects of Syzygium samarangense, Passiflora edulis and Solanum muricatum on alcohol-induced liver injury in mice. The animals were treated daily with alcohol and fruit juices for fifteen days. Chronic treatment with alcohol increased the levels of aspartate transaminase (AST), alanine transaminase (ALT), total bilirubin (TBIL), triglyceride (TG), malondialdehyde (MDA), and decreased total protein (TP). Histopathological evaluation also showed that ethanol induced extensive fat droplets in hepatocyte cytoplasm. Syzygium samarangense and Passiflora edulis normalized various biochemical parameters. Solanum muricatum increased the level of ALT and induced infiltration of inflammatory cells in the liver. These results strongly suggest that treatment with Syzygium samarangense and Passiflora edulis could protect liver from the injury of alcohol, while Solanum muricatum could aggravate the damage.

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Agrimonia eupatoria protects against chronic ethanol-induced liver injury in rats

Cited by 52 publications

References 38 publications

Saponins isolated from the leaves of Panax notoginseng protect against alcoholic liver injury via inhibiting ethanol-induced oxidative stress and gut-derived endotoxin-mediated inflammation

Saponins isolated from the leaves of Panax notoginseng protect against alcoholic liver injury via inhibiting ethanol-induced oxidative stress and gut-derived endotoxin-mediated inflammation

Retraction: Asiatic Acid from <i>Potentilla chinensis</i> Attenuate Ethanol-Induced Hepatic Injury <i>via</i> Suppression of Oxidative Stress and Kupffer Cell Activation

The Effects of Syzygium samarangense, Passiflora edulis and Solanum muricatum on Alcohol-Induced Liver Injury

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