Agrimonia pilosa ethanol extract induces apoptotic cell death in HepG2 cells

Nho, Kyoung Jin; Chun, Jin Mi; Kim, Ho Kyoung

doi:10.1016/j.jep.2011.09.008

Cited by 28 publications

(18 citation statements)

References 29 publications

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“…Previous scientific investigations focused on hypoglycaemic (Gray & Flatt 1998), coronary (Correia et al 2006), anti-tumour (Nho et al 2011), antioxidant (Kubínová et al 2016) and other activities of ethanol extracts and water extracts of genus Agrimonia. However, there are few researches on the active components of the species from Agrimonia, not to say the systematic research on the chemical composition.…”

Section: Introductionmentioning

confidence: 99%

Chemical constituents from Agrimonia pilosa Ledeb. and their chemotaxonomic significance

Liu

Hou

Chen

et al. 2016

Natural Product Research

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Phytochemical investigation of the ethanol extract from the whole plant of Agrimonia pilosa led to the isolation of 31 compounds, including 16 flavonoids (1-16), 5 triterpenes (17-21), 1 isocoumarin (22), 5 phenolic acids (23-27), 1 ceramide (28), 2 agrimols (29-30) and 1 fatty acid (31). Their structures were determined by various spectroscopic analyses. Compounds 5, 7 and 20 were firstly isolated from the genus Agrimonia, and compounds 6, 10-11, 15, 26, 28 and 31 were isolated from the family Rosaceae for the first time. Moreover, the chemotaxonomic significance of these compounds was summarised.

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Section: Introductionmentioning

confidence: 99%

Chemical constituents from Agrimonia pilosa Ledeb. and their chemotaxonomic significance

Liu

Hou

Chen

et al. 2016

Natural Product Research

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“…29 In this study, the activity of Na Study has shown that Agrimonia pilosa has the function of inducing apoptosis. 33 In the present study, we use apoptosis-related genes P53 and HSP70 as the key indicators to monitor the effects of different extracts of Agrimonia pilosa on regulating their expression in cerebral ischemia-reperfusion injury, and explore the neuroprotective function and mechanisms of Agrimonia pilosa in cerebral ischemic injury.…”

Section: Discussionmentioning

confidence: 99%

Experimental Study on the Protection of Agrimony Extracts from Different Extracting Methods against Cerebral Ischemia-Reperfusion Injury△

Zhu

Bie

Wang

et al. 2017

Chinese Medical Sciences Journal

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Objective To study the protective effect of agrimony extracts from different extracting methods on cerebral ischemia-reperfusion injury in rats, in order to optimize the extraction scheme of agrimony.Methods Male rats were randomly assigned into seven groups: 1. Sham-operated group, 2. Untreated MCAO group (MCAO), 3. Petroleum ether extract of Agrimonia pilosa treated MCAO group (PEA), 4. Ethyl acetate extract of Agrimonia pilosa treated MCAO group (EAEA), 5. Ethanol extract of Agrimonia pilosa treated MCAO group (EEA), 6. Water extract of Agrimonia pilosa treated MCAO group (WEA), 7. Nimodipine treated MCAO group (NP). Intragastrical drug administration (i.g) was performed at 0 and 6 hours after MCAO. Neurological function tests were performed after reperfusion for 24 hours, then the brain was removed for the evaluations of the cerebral infarction volume (percentage of total brain volume) by immunohistochemistry, histological changes (hematoxylin-eosin staining), Na + /K + -ATPase, Ca 2+ -ATPase (modified method of Svoboda and Mosinger), mRNA expression of Tumor suppressor gene (P53) and hot shock protein (HSP70) (quantitative real-time PCR).Results The neurological function of MCAO group had significantly higher scores than the sham group (P<0.01). The WEA group showed a significantly lower neurological score than the MCAO group (P<0.05), indicating the protective effect of WEA on neurological deficits. The mean infarction volumes of WEA (13.5±6. 6%, F=4.75, P<0.01), EEA (19.90±6.90%, F=5.23, P<0.01), PEA (20.40±5.30%, F=4.68, P<0.01) and EAEA (22.50±10.50%, F=6.25, P<0.05) group were all significantly smaller than that of MCAO group (29.40±6.50%). HE staining demonstrated that, compared to the treated groups, the infarcted cerebral

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“…The accumulation of cells in the sub-G 1 phase is an apoptotic characteristic and may be determined using a fluorescence-activated cell sorting (FACS) method, as previously described (28)(29)(30). Briefly, control (DBTRG cells without RC-RNase treatment) and experimental cells (DBTRG cells with 20 ug/ml RC-RNase treatment) were fixed with 70% alcohol at 4˚C for 1 h. The fixed cells were washed with phosphate-buffered saline (PBS) and treated with 1 ml propidium iodide (PI) solution, containing 50 µg/ml PI, 100 µg/ml RNase A and 0.1% Triton X-100, at 37˚C for 1 h. Following the PBS wash, these cells were analyzed by flow cytometry, which was performed using the CyFlow SL flow cytometer and Flomax software from Sysmex Partec GmbH (Görlitz, Görlitz, Germany).…”

Section: Methodsmentioning

confidence: 99%

“…Therefore, RC-RNase may induce apoptotic cell death in DBTRG cells. Additionally, the apoptotic characteristic of cells accumulating in the sub-G 1 phase was identified following 72 h of treatment in the RC-RNase-treated DBTRG cells, which was determined using a FACS analysis method as previously described (28)(29)(30). As Fig.…”

Section: Rc-r Nase Induces Apoptosis a Nd Activation Of Caspases-9 Anmentioning

confidence: 99%

Rana catesbeiana ribonuclease induces cell apoptosis via the caspase-9/-3 signaling pathway in human glioblastoma DBTRG, GBM8901 and GBM8401 cell lines

Chen¹,

Yiang²,

Lin³

et al. 2015

Oncology Letters

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Abstract. Human glioblastoma multiforme is one of the most aggressive malignant brain tumor types, and the mean survival time of patients with a brain tumor is <2 years when traditional therapies are administered. Thus, numerous studies have focused on the development of novel treatments for brain tumors. Frog ribonucleases, such as Onconase and Rana catesbeiana ribonuclease (RC-RNase), exert antitumor effects on various tumor cells, including cervical cancer, breast cancer, hepatoma, leukemia, pancreatic cancer and prostate cancer cells. In addition, frog Onconase has been applied as a treatment in clinical trials. However, the antitumor effects of frog ribonucleases on brain tumors are unclear. Previous studies have indicated that RC-RNase demonstrates a decreased cytotoxic effect in normal cells compared with Onconase. Therefore, the present study investigated the ability of RC-RNase to exert antitumor activities on human glioblastoma.It was found that RC-RNase inhibits the growth of the human glioblastoma DBTRG, GBM8901 and GBM8401 cells. In addition, the present study revealed that RC-RNase induces caspase-9/-3 activity and triggers the apoptotic cell death pathway in human glioblastoma cells. Notably, it was also demonstrated that RC-RNase effectively inhibits the growth of human glioblastoma tumors in a nude mouse model. Overall, the present study indicates that RC-RNase may be a potential agent for the treatment of human glioblastoma.

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Agrimonia pilosa ethanol extract induces apoptotic cell death in HepG2 cells

Cited by 28 publications

References 29 publications

Chemical constituents from Agrimonia pilosa Ledeb. and their chemotaxonomic significance

Chemical constituents from Agrimonia pilosa Ledeb. and their chemotaxonomic significance

Experimental Study on the Protection of Agrimony Extracts from Different Extracting Methods against Cerebral Ischemia-Reperfusion Injury△

Rana catesbeiana ribonuclease induces cell apoptosis via the caspase-9/-3 signaling pathway in human glioblastoma DBTRG, GBM8901 and GBM8401 cell lines

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