The production of amphiphilic chemicals, known as biosurfactants, by different microorganisms is essential for lowering interfacial and surface tension. The pathways of biosurfactant synthesis are examined in this review, with a focus on the influence of carbon substrates on biosynthesis. It also covers the variety of substrates used in the production of biosurfactants, such as lignocellulosic wastes, agro-industrial wastes, oil processing byproducts, starch-rich wastes, industrial wastes derived from plants and animals, fish and chicken wastes, fruit and vegetable wastes, and wastes from fish and poultry operations. In addition, the optimization of media for the formation of biosurfactants is discussed, with particular attention to temperature, pH, concentration of salt, and sources of carbon and nitrogen. It is crucial to comprehend these routes and optimization techniques in order to address the economic and environmental issues related to waste management and increase the efficiency of biosurfactant production.