Agro-industrial wastes utilization for the generation of fibrinolytic metalloprotease by Serratia marcescens RSPB11

Scale-Up of the Fermentation Process for the Production and Purification of Serratiopeptidase Using Silkworm Pupae as a Substrate

“…Time also positively contributed to proteolytic activity, albeit to a lesser extent (a coefficient of 32), whereas temperature exerted a negative influence on proteolytic activity. This contradicts the dynamics of serratiopeptidase production, where elevated temperatures adversely impact Serratia marcescens bacteria, leading to a diminished enzyme yield [ 7 ].…”

“…The fermentation process was optimized at the agitation flask level through a Taguchi experimental design to achieve the optimal combination of conditions for serratiopeptidase enzyme production [ 7 ]. The study evaluated the relative importance of four variables, namely, silkworm pupa abundance, temperature, time, and pH.…”

“…One of the strategies for optimizing factors affecting fermentation involves classical optimization methods, such as modifying one factor at a time (OFAT) [ 5 , 6 ]. However, in the conventional approach, assessing the role of each parameter and its influence on product performance is difficult, laborious, slow, and costly [ 7 ]. Therefore, statistical methods such as experimental design (DOE) are powerful tools for optimizing fermentation processes [ 6 ].…”

“…Among the various optimization tools, the Taguchi method can facilitate the simultaneous optimization of multiple factors and generate quantitative data through fewer experimental trials. This method enhances the reproducibility and efficiency of the process by reducing experimental errors through a set of experimental conditions known as orthogonal arrays, where each phase has a separate yet interconnected objective aimed at achieving the optimization process [ 7 , 10 ]. The successful application of this method for optimizing fermentation process parameters in the production of proteases has been demonstrated [ 7 , 11 , 12 ].…”

“…This enzyme is renowned for its anti-inflammatory, fibrinolytic, antibiofilm, and analgesic effects [ 20 , 21 , 22 , 23 , 24 , 25 ]. The production of serratiopeptidase has been reported using various agro-industrial residues, such as wheat husk and rice bran [ 7 ]. Our research group has reported the production of serratiopeptidase using silkworm pupae as a source of carbon and nitrogen [ 26 ].…”