Agrobacterium-mediated co-transformation of multiple genes in upland cotton

Li, Feifei; Wu, Shengyuan; Chen, Tianzi; Zhang, Jie; Wang, Haihai; Guo, Wangzhen; Zhang, Tianzhen

doi:10.1007/s11240-009-9521-2

Cited by 41 publications

(20 citation statements)

References 43 publications

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“…Cotton transformation was performed as previously described (Li et al 2009). Genomic DNA was isolated from young leaves of non-transgenic and transgenic cotton plantlets following the protocol of Paterson et al (1993).…”

Section: Plant Transformation and Generation Of Ghann1 Transgenic Cotmentioning

confidence: 99%

RETRACTED ARTICLE: Overexpression of a cotton annexin gene, GhAnn1, enhances drought and salt stress tolerance in transgenic cotton

Zhang

Yang

et al. 2014

Plant Mol Biol

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Plant annexins are members of a diverse, multigene protein family that has been associated with a variety of cellular processes and responses to abiotic stresses. GhAnn1, which encodes a putative annexin protein, was isolated from a cotton (Gossypium hirsutum L. acc 7235) cDNA library. Tissue-specific expression showed that GhAnn1 is expressed at differential levels in all tissues examined and strongly induced by various phytohormones and abiotic stress. In vivo and in vitro subcellular localization suggested that GhAnn1 is located in the plasma membrane. In response to drought and salt stress, transgenic cotton plants overexpressing GhAnn1 showed significantly higher germination rates, longer roots, and more vigorous growth than wild-type plants. In addition, plants overexpressing GhAnn1 had higher total chlorophyll content, lower lipid peroxidation levels, increased peroxidase activities, and higher levels of proline and soluble sugars, all of which contributed to increased salt and drought stress tolerance. However, transgenic cotton plants in which the expression of GhAnn1 was suppressed showed the opposite results compared to the overexpressing plants. These findings demonstrated that GhAnn1 plays an important role in the abiotic stress response, and that overexpression of GhAnn1 in transgenic cotton improves salt and drought tolerance.

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Section: Plant Transformation and Generation Of Ghann1 Transgenic Cotmentioning

confidence: 99%

RETRACTED ARTICLE: Overexpression of a cotton annexin gene, GhAnn1, enhances drought and salt stress tolerance in transgenic cotton

Zhang

Yang

et al. 2014

Plant Mol Biol

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“…The PCR product in the antisense orientation was subcloned into pBI121 and pBI121–E6 in which the CaMV 35S promoter was replaced by the GaE6 promoter (our unpublished data) by digestion with Sma I and Sac I to replace the GUS gene downstream of the CaMV 35S or E6 promoters, respectively. These two vectors were introduced into the cotton line W0 via Agrobacterium ‐mediated transformation (Figure S6) (Li et al. , 2009).…”

Section: Resultsmentioning

confidence: 99%

Overexpression of GhSusA1 increases plant biomass and improves cotton fiber yield and quality

Jiang

Guo

Zhu

et al. 2011

Plant Biotechnology Journal

144

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SummaryCotton (Gossypium spp.) is an important economic crop and the largest source of textile fiber in the world. However, to date, only a few genes have been identified that exhibit critical roles in fiber development, and few has shown positive effects on fiber yield and quality in transgenic cotton. Here, we report the characterization of a novel sucrose synthase (SusA1) gene from a superior quality fiber germplasm line 7235 in Gossypium hirsutum. By association analysis, GhSusA1 was highly correlated with fiber qualities in (7235· TM-1) recombinant inbred lines based on polymorphism of GhSusA1 between 7235 and TM-1. Subsequently, based on an interspecific population of 141 BC 1 individuals generated from the cross between TM-1 and Gossypium barbadense line, Hai7124, we further mapped GhSusA1 genes on homeologous chromosomes A8 (chro.8) and D8 (chro.24). Suppression of GhSusA1 in transgenic cotton reduced fiber quality and decreased the boll size and seed weight. Importantly, overexpression of this gene increased fiber length and strength, with the latter indicated by the enhanced thickening of cell wall during secondary wall formation stage. Moreover, increasing GhSusA1 transcript abundance in vegetative tissues led to elevated seedling biomass. Together, these findings identified GhSusA1 as a key regulator of sink strength in cotton, which is tightly associated with productivity, and hence a promising candidate gene that can be developed to increase cotton fiber yield and quality.

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“…Cotton transformation was carried out as described [ 24 ]. To construct the overexpression and antisense vectors, two pairs of primers containing Xba I and Sma I sites were used to amplify GhCPC and the PCR products were ligated into pBI121 vectors, respectively.…”

Section: Methodsmentioning

confidence: 99%

The R3-MYB Gene GhCPC Negatively Regulates Cotton Fiber Elongation

2015

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Cotton (Gossypium spp.) fibers are single-cell trichomes that arise from the outer epidermal layer of seed coat. Here, we isolated a R3-MYB gene GhCPC, identified by cDNA microarray analysis. The only conserved R3 motif and different expression between TM-1 and fuzzless-lintless mutants suggested that it might be a negative regulator in fiber development. Transgenic evidence showed that GhCPC overexpression not only delayed fiber initiation but also led to significant decreases in fiber length. Interestingly, Yeast two-hybrid analysis revealed an interaction complex, in which GhCPC and GhTTG1/4 separately interacted with GhMYC1. In transgenic plants, Q-PCR analysis showed that GhHOX3 (GL2) and GhRDL1 were significantly down regulated in −1–5 DPA ovules and fibers. In addition, Yeast one-hybrid analysis demonstrated that GhMYC1 could bind to the E-box cis-elements and the promoter of GhHOX3. These results suggested that GhHOX3 (GL2) might be downstream gene of the regulatory complex. Also, overexpression of GhCPC in tobacco led to differential loss of pigmentation. Taken together, the results suggested that GhCPC might negatively regulate cotton fiber initiation and early elongation by a potential CPC-MYC1-TTG1/4 complex. Although the fibers were shorter in transgenic cotton lines than in the wild type, no significant difference was detected in stem or leaf trichomes, even in cotton mutants (five naked seed or fuzzless), suggesting that fiber and trichome development might be regulated by two sets of genes sharing a similar model.

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Agrobacterium-mediated co-transformation of multiple genes in upland cotton

Cited by 41 publications

References 43 publications

RETRACTED ARTICLE: Overexpression of a cotton annexin gene, GhAnn1, enhances drought and salt stress tolerance in transgenic cotton

RETRACTED ARTICLE: Overexpression of a cotton annexin gene, GhAnn1, enhances drought and salt stress tolerance in transgenic cotton

Overexpression of GhSusA1 increases plant biomass and improves cotton fiber yield and quality

The R3-MYB Gene GhCPC Negatively Regulates Cotton Fiber Elongation

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