Agrobacterium-mediated genetic transformation and cloning of candidate reference genes in suspension cells of Artemisia pallens Wall. ex DC

Jogam, Phanikanth; Sandhya, Dulam; Alok, Anshu; Shekhawat, Mahipal S.; Peddaboina, Venkataiah; Singh, Kashmir; Allini, Venkateswar Rao

doi:10.1007/s13205-022-03251-x

3 Biotech

2022

DOI: 10.1007/s13205-022-03251-x

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Agrobacterium-mediated genetic transformation and cloning of candidate reference genes in suspension cells of Artemisia pallens Wall. ex DC

Phanikanth Jogam

Dulam Sandhya

Anshu Alok

et al.

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2024

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Cited by 2 publications

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Optimizing the Genetic Transformation of Coffea arabica Using Agrobacterium tumefaciens

Molina,

Acuña

2024

IJPB

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The genetic transformation of Coffea arabica L. is an alternative strategy for obtaining plants with agronomic traits of interest that is less time-consuming than conventional breeding methods. Given the importance of coffee cultivation in Colombia, this study evaluated the main factors interfering with the genetic transformation of C. arabica using Agrobacterium tumefaciens. An efficient and reproducible method was accordingly developed that involved propagating “early” embryogenic calli in a liquid proliferation medium supplemented with 3 mg L−1 BAP for eight months, followed by sonication for 300 s in a suspension of LBA4404 OD600 of 0.5, harboring pCambia1301, and then incubation in this same suspension for 1 h. The vector pCambia1301 contained the uidA gene under control of the 35S promoter. A micropipette was used to remove the Agrobacterium suspension from the embryogenic callus. The remaining Agrobacterium suspension was blotted off by placing the embryogenic callus on filter paper. The embryogenic callus was then co-cultured for four days in a solid differentiation medium supplemented with 100 µM acetosyringone on filter paper. Subsequently, the embryogenic callus was post-cultured for four days in liquid differentiation medium under constant shaking at 100 rpm with 300 mg L−1 Cefotaxime, followed by selection with 50 mg L−1 hygromycin at 26 °C in the dark, with subcultures at 20-day intervals until somatic embryos were formed for subsequent culturing in germination medium. Molecular analysis confirmed the presence of the uidA gene in coffee seedlings transformed with strains LBA4404 and EHA105 and vectors pCambia1301 and pCambia2301 by polymerase chain reaction (PCR) analysis. This method successfully enables the stable integration of genes of interest in the coffee plant genome.

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Optimizing the Genetic Transformation of Coffea arabica Using Agrobacterium tumefaciens

Molina,

Acuña

2024

IJPB

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Artemisia pallens Wall. ex. DC: A comprehensive review

Yogendra,

Kumara,

Prakash

et al. 2024

J Herbmed Pharmacol

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Davana (Artemisia pallens Wall. ex DC) is a well-known and precious essential oil-bearing plant under the family Compositae (Asteraceae). It is an erectly grown herbaceous plant exclusively cultivated as a short-term crop in south India during winter. It is distributed in the northern hemisphere in subtropical Africa, South Africa, West America, and South America; however, it is indigenous to southern India. The yearly productivity of davana oil typically falls between 8 to 10 tones. Davana oil is mainly used to flavor pastries, cakes, tobacco, and some beverages. It consists of diverse phyto-molecules and is an important and unique commodity in the fragrance, flavor, and liquor industries. It has excellent biological potential and contains volatile and nonvolatile constituents such as terpenoids, flavonoids, and alkaloids, which have high pharmacological values. Several investigations have focused on validating the importance of chemical constituents and the biological efficacy of essential oils and various extracts in multiple industries. The review focuses on the complete agronomic practices, botany, breeding, biotechnology, and chemistry of davana essential oil (DEO), viz, volatile and nonvolatile constituents, phytomedicinal and pharmacological properties, which may help researchers achieve their future goals for approaching its industrial or commercial viability as a natural product ingredient.

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Agrobacterium-mediated genetic transformation and cloning of candidate reference genes in suspension cells of Artemisia pallens Wall. ex DC

Cited by 2 publications

References 57 publications

Optimizing the Genetic Transformation of Coffea arabica Using Agrobacterium tumefaciens

Optimizing the Genetic Transformation of Coffea arabica Using Agrobacterium tumefaciens

Artemisia pallens Wall. ex. DC: A comprehensive review

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