Agrobacterium-Mediated Genetic Transformation of Cotton

Rajasekaran, Kanniah

doi:10.1007/978-1-4020-2333-0_18

Cited by 20 publications

(3 citation statements)

References 23 publications

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“…Agrobacterium ‐mediated transformation of seedling explants of cotton ( Gossypium hirsutum L. cv. Coker 312) and regeneration of fertile, transgenic plants were carried out according to the published protocols (Rajasekaran et al ., 1996; Rajasekaran, 2004). Plants transformed with the pBI‐d35SΩ‐ uid A‐nos construct (CGUS) served as controls.…”

Section: Methodsmentioning

confidence: 99%

Disease resistance conferred by the expression of a gene encoding a synthetic peptide in transgenic cotton (Gossypium hirsutum L.) plants

Rajasekaran

Cary

Jaynes

et al. 2005

Plant Biotechnology Journal

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SummaryFertile, transgenic cotton plants expressing the synthetic antimicrobial peptide, D4E1, were produced through Agrobacterium -mediated transformation. PCR products and Southern blots confirmed integration of the D4E1 gene, while RT-PCR of cotton RNA confirmed the presence of D4E1 transcripts. In vitro assays with crude leaf protein extracts from T0 and T1 plants confirmed that D4E1 was expressed at sufficient levels to inhibit the growth of Fusarium verticillioides and Verticillium dahliae compared to extracts from negative control plants transformed with pBI-d35S Ω -uid A-nos (CGUS). Although in vitro assays did not show control of pre-germinated spores of Aspergillus flavus , bioassays with cotton seeds in situ or in planta , inoculated with a GFP-expressing A. flavus , indicated that the transgenic cotton seeds inhibited extensive colonization and spread by the fungus in cotyledons and seed coats. In planta assays with the fungal pathogen, Thielaviopsis basicola, which causes black root rot in cotton, showed typical symptoms such as black discoloration and constriction on hypocotyls, reduced branching of roots in CGUS negative control T1 seedlings, while transgenic T1 seedlings showed a significant reduction in disease symptoms and increased seedling fresh weight, demonstrating tolerance to the fungal pathogen. Significant advantages of synthetic peptides in developing transgenic crop plants that are resistant to diseases and mycotoxin-causing fungal pathogens are highlighted in this report.

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Section: Methodsmentioning

confidence: 99%

Disease resistance conferred by the expression of a gene encoding a synthetic peptide in transgenic cotton (Gossypium hirsutum L.) plants

Rajasekaran

Cary

Jaynes

et al. 2005

Plant Biotechnology Journal

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“…Identification of specific genes and critical genetic pathways is essential for conventional and transgenic improvement programs. Analysis of gene function in native plant environment is possible using efficient genetic transformation technology and it has been practised routinely for cotton, peanut and corn (Rajasekaran 2004;Ozias-Akins et al, 1993;Ishida et al, 2007). In conventional breeding, MAS may not be preferable for traits controlled by a large number of QTLs, because the individual gene's contribution may be too small to measure since many genes with small effects are involved (Morgante and Salamini, 2003).…”

Section: Genomics Of Crop Plants Infected By Aspergillus Flavusmentioning

confidence: 99%

The 'omics' tools: genomics, proteomics, metabolomics and their potential for solving the aflatoxin contamination problem

Bhatnagar

Rajasekaran

Payne

et al. 2008

WMJ

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Aflatoxins are highly carcinogenic secondary metabolites produced primarily by the fungi Aspergillus flavus and Aspergillus parasiticus. Aflatoxin contamination of food and feed is an age old problem of particular concern over the last four decades. Now, for the first time control measures for this problem appear within reach. For practical and sustainable control of aflatoxin contamination to be realised, however, additional information is needed rather rapidly, particularly for understanding the specific molecular factors (both in the plant and the fungus) involved during host plant-fungus interaction. The information derived from the use of novel tools such as genomics, proteomics and metabolomics provides us with the best and the quickest opportunity to achieve a clear understanding of the survival of toxigenic fungi in the field, the ability of the fungus to invade crops, and the process of toxin contamination under various environmental conditions. Significant progress has been made recently in understanding the genomic makeup of the most significant aflatoxin producing field fungus, namely Aspergillus flavus. Progress also has been made in the study of host crop resistance to fungal invasion through the use of proteomics. The information available on production of aflatoxin and other metabolites by Aspergillus flavus is reasonably extensive, although the application of metabolomics as a tool in this study is relatively new. In this review there is a discussion of the use of genomics, proteomics and metabolomics in deriving the requisite information for developing effective strategies to interrupt the machinery in the fungus for production of these toxins, as well as to assist in the development of host-resistance against fungal invasion and aflatoxin contamination of crops.

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“…GA98033 is embryogenic and has regeneration frequency from somatic embryos into plantlets similar to that of Coker 312 (Sakhanokho et al, 2004), a feature that may permit utility as a recipient of transgenic traits. Although the range of germplasm capable of embryogenesis has expanded in recent years, almost all commercial applications of cotton transformation are still performed in Coker 312 or close relatives (Rajasekaran et al, 2001). An embryogenic germplasm with yield potential commensurate or exceeding that of many currently popular cultivars could have value as a donor parent for new transgenic traits, compared with the agronomic potential of Coker 312 that was released in 1972 (Calhoun et al, 1997).…”

Section: Acknowledgmentsmentioning

confidence: 99%