Agrobacterium-Mediated Genetic Transformation of Grapevines: Transgenic Plants of Vitis rupestris SCHEELE and Buds of Vitis vinifera L.

Mullins, M. G.; Tang, Fangping; Facciotti, Daniel

doi:10.1038/nbt1190-1041

Cited by 99 publications

(50 citation statements)

References 25 publications

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“…However, the frequency of transformation was significantly higher using a linear GUS/BAR gene than the whole plasmid (Uzé et al, 1999;Yao et al, 2006). The low transformation frequency might due to the absence of selective pressure, without which transformed cells tend to be disadvantageous in competition with non-transformed cells and consequently die more (Mullins et al, 1990).…”

Section: Discussionmentioning

confidence: 92%

Biolistic transformation of wheat using the HMW-GS 1Dx5 gene without selectable markers

Qin¹,

Wang²,

Zhu³

2014

Genet. Mol. Res.

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ABSTRACT. Genetic manipulation using linear DNA was applied to the common wheat variety Xindong No. 26 via particle bombardment with the aim to improve bread-making quality of flour. Initially, 2 biolistic parameters, helium pressure and target distance, were optimized using plasmid pAHC25. We transformed wheat immature embryo scutella with the linear 1Dx5 gene without selectable markers. The highest transient β-glucuronidase expression was obtained when scuttles were bombarded at 1100 psi with a 9-cm target distance. Using the optimized parameters, the transformation of the common wheat variety Xindong No. 26 was carried out using the linear 1Dx5. Three transgenic plants were identified from 1003 transgenic plants, yielding a transformation frequency of 0.4%. A sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis confirmed that the 1Dx5 gene was expressed in 4 T1 seeds of the transgenic plants. These experiments indicate the possibility of obtaining marker-free transgenic wheat plants via particle bombardment using the minimal gene cassettes with the particle bombardment parameters.

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Section: Discussionmentioning

confidence: 92%

Biolistic transformation of wheat using the HMW-GS 1Dx5 gene without selectable markers

Qin¹,

Wang²,

Zhu³

2014

Genet. Mol. Res.

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“…Further, inclusion of feeder plates during cocultivation has been found to exert a positive influence on transformation and regeneration (Cervera et al, 1998c) as well as in other species such as grapevine (Mullins et al, 1990) and kiwifruit (Janssen and Gardner, 1993), possibly by permitting vir-activating compounds through them into the explants (Horsch et al, 1985;Fillatti et al, 1987a, b). After successful transformation, the rooting of putative transgenic shoots is no less problematic, as the young shoots are small and weak and compared to synthetic media, they tend to root better in soil (Moore et al, 1992).…”

Section: Agrobacterium-mediated Transformationmentioning

confidence: 99%

Citrus biotechnology: Achievements, limitations and future directions

Singh

Rajam

2009

Physiol Mol Biol Plants

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Citrus is one of the most important commercial and nutritional fruit crops in the world, hence it needs to be improved to cater to the diverse needs of consumers and crop breeders. Genetic manipulation through conventional techniques in this genus is invariably a difficult task for plant breeders as it poses various biological limitations comprising long juvenile period, high heterozygosity, sexual incompatibility, nucellar polyembryony and large plant size that greatly hinder cultivar improvement. Hence, several attempts were made to improve Citrus sps. by using various in vitro techniques. Citrus sps are widely known for their recalcitrance to transformation and subsequent rooting, but constant research has led to the establishment of improved protocols to ensure the production of uniformly transformed plants, albeit with relatively low efficiency, depending upon the genotype. Genetic modification through Agrobacterium-mediated transformation has emerged as an important tool for introducing agronomically important genes into Citrus sps. Somatic hybridization has been applied to overcome self and cross-incompatibility barriers and generated inter-specific and inter-generic hybrids. Encouraging results have been achieved through transgenics for resistance against viruses and bacteria, thereby augmenting the yield and quality of the fruit. Now, when major transformation and regeneration protocols have sufficiently been standardized for important cultivars, ongoing citrus research focuses mainly on incorporating such genes in citrus genotypes that can combat different biotic and abiotic stresses. This review summarizes the advances made so far in Citrus biotechnology, and suggests some future directions of research in this fruit crop.

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“…There was even an analytical test to find if a wine contains the product of the HPD. Restrictions imposed by the governing boards of wine and wine grape "VPQRD" rule out genetic modification of conventional wine grape genotypes, whether by traditional breeding methods or by molecular genetic approaches [3]. The direct producer hybrids obtained from Vitis vinifera L. and North American species and therefore have no place in the wine growing areas classified as "AOC".…”

Section: Introductionmentioning

confidence: 99%

Rooting Abilities in <i>Vitis vinifera</i> L.: Total Lipid and Fatty Acids Evolution in Perle Noir Cutting

Cheikhrouhou¹,

Zrida²,

Ezzili³

2015

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Rooting ability in Vitis vinifera L. is the phenomena studied. The evolution of the total lipids contents and fatty acids amount of cuttings during the rooting were determined. Total lipid extraction was carried out by an extraction solvent consisting of a mixture of chloroform and methanol. Methylation of fatty acids was carried out by the boron trifluoride methanol complex. Their analysis is performed by gas chromatography. We have obtained a content of merithallus lipids equivalent with then in bibliography. In other hand 3 fatty acids were obtained: eicosapentaenoic acid, oleic acid and docosanoic acid. Among the studied of rooting abilities, we observed decreased levels of total lipids and in the essential fatty acids: eicosapentaenoic acid C20:5 n-3 and oleic acid C18:1 n-9. Minor fatty acids cuttings experimental have a variable evolution. Lipids and certain fatty acids may be markers of rooting in the vine.

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Agrobacterium-Mediated Genetic Transformation of Grapevines: Transgenic Plants of Vitis rupestris SCHEELE and Buds of Vitis vinifera L.

Cited by 99 publications

References 25 publications

Biolistic transformation of wheat using the HMW-GS 1Dx5 gene without selectable markers

Biolistic transformation of wheat using the HMW-GS 1Dx5 gene without selectable markers

Citrus biotechnology: Achievements, limitations and future directions

Rooting Abilities in <i>Vitis vinifera</i> L.: Total Lipid and Fatty Acids Evolution in Perle Noir Cutting

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Agrobacterium-Mediated Genetic Transformation of Grapevines: Transgenic Plants of Vitis rupestris SCHEELE and Buds of Vitis vinifera L.

Cited by 99 publications

References 25 publications

Biolistic transformation of wheat using the HMW-GS 1Dx5 gene without selectable markers

Biolistic transformation of wheat using the HMW-GS 1Dx5 gene without selectable markers

Citrus biotechnology: Achievements, limitations and future directions

Rooting Abilities in &lt;i&gt;Vitis vinifera&lt;/i&gt; L.: Total Lipid and Fatty Acids Evolution in Perle Noir Cutting

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Rooting Abilities in <i>Vitis vinifera</i> L.: Total Lipid and Fatty Acids Evolution in Perle Noir Cutting