Agrobacterium Mediated Genetic Transformation of Chrysanthemum (Dendranthema grandiflora Tzvelev) with Rice Chitinase Gene for Improved Resistance Against Septoria obesa

Sen, S. P.; Kumar, Surinder; Ghani, Minerva; Thakur, Manisha

doi:10.3923/ppj.2013.1.10

Cited by 13 publications

(5 citation statements)

References 18 publications

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“…Overexpression of the hrp gene, hpaG X00 , imparted resistance to Alternaria tenuissima in chrysanthemum [ 136 ]. Introduction of rice chitinase gene ( chiII ) enhanced resistance to Septoria obesa -caused leaf spot in chrysanthemum ‘Snowball’ [ 137 ]. Transcriptome analysis in response to black spot disease caused by Alternaria sp.…”

Section: Biotic Stress Resistancementioning

confidence: 99%

Towards the Improvement of Ornamental Attributes in Chrysanthemum: Recent Progress in Biotechnological Advances

Mekapogu

Kwon

Song

et al. 2022

IJMS

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Incessant development and introduction of novel cultivars with improved floral attributes are vital in the dynamic ornamental industry. Chrysanthemum (Chrysanthemum morifolium) is a highly favored ornamental plant, ranking second globally in the cut flower trade, after rose. Development of new chrysanthemum cultivars with improved and innovative modifications in ornamental attributes, including floral color, shape, plant architecture, flowering time, enhanced shelf life, and biotic and abiotic stress tolerance, is a major goal in chrysanthemum breeding. Despite being an economically important ornamental plant, the application of conventional and molecular breeding approaches to various key traits of chrysanthemum is hindered owing to its genomic complexity, heterozygosity, and limited gene pool availability. Although classical breeding of chrysanthemum has resulted in the development of several hundreds of cultivars with various morphological variations, the genetic and transcriptional control of various important ornamental traits remains unclear. The coveted blue colored flowers of chrysanthemums cannot be achieved through conventional breeding and mutation breeding due to technical limitations. However, blue-hued flower has been developed by genetic engineering, and transgenic molecular breeding has been successfully employed, leading to substantial progress in improving various traits. The recent availability of whole-genome sequences of chrysanthemum offers a platform to extensively employ MAS to identify a large number of markers for QTL mapping, and GWAS to dissect the genetic control of complex traits. The combination of NGS, multi-omic platforms, and genome editing technologies has provided a tremendous scope to decipher the molecular and regulatory mechanisms. However, the application and integration of these technologies remain inadequate for chrysanthemum. This review, therefore, details the significance of floral attributes, describes the efforts of recent advancements, and highlights the possibilities for future application towards the improvement of crucial ornamental traits in the globally popular chrysanthemum plant.

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Section: Biotic Stress Resistancementioning

confidence: 99%

Towards the Improvement of Ornamental Attributes in Chrysanthemum: Recent Progress in Biotechnological Advances

Mekapogu

Kwon

Song

et al. 2022

IJMS

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“…Overexpression of one such hrp gene, hpaG Xoo , conferred increased resistance to Alternaria tenuissima in chrysanthemums [ 101 ], and the introduction of the rice chitinase gene ( chiII ) in chrysanthemums cv. Snowball resulted in increased resistance to leaf spot caused by Septoria obesa [ 102 ]. Gray mold disease caused by B. cinerea is the predominant fungal disease in chrysanthemums.…”

Section: Genetic Engineering For Improved Fungal Disease Resistance In Ornamental Plantsmentioning

confidence: 99%

Recent Progress in Enhancing Fungal Disease Resistance in Ornamental Plants

Mekapogu

Jung

Kwon

et al. 2021

IJMS

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Fungal diseases pose a major threat to ornamental plants, with an increasing percentage of pathogen-driven host losses. In ornamental plants, management of the majority of fungal diseases primarily depends upon chemical control methods that are often non-specific. Host basal resistance, which is deficient in many ornamental plants, plays a key role in combating diseases. Despite their economic importance, conventional and molecular breeding approaches in ornamental plants to facilitate disease resistance are lagging, and this is predominantly due to their complex genomes, limited availability of gene pools, and degree of heterozygosity. Although genetic engineering in ornamental plants offers feasible methods to overcome the intrinsic barriers of classical breeding, achievements have mainly been reported only in regard to the modification of floral attributes in ornamentals. The unavailability of transformation protocols and candidate gene resources for several ornamental crops presents an obstacle for tackling the functional studies on disease resistance. Recently, multiomics technologies, in combination with genome editing tools, have provided shortcuts to examine the molecular and genetic regulatory mechanisms underlying fungal disease resistance, ultimately leading to the subsequent advances in the development of novel cultivars with desired fungal disease-resistant traits, in ornamental crops. Although fungal diseases constitute the majority of ornamental plant diseases, a comprehensive overview of this highly important fungal disease resistance seems to be insufficient in the field of ornamental horticulture. Hence, in this review, we highlight the representative mechanisms of the fungal infection-related resistance to pathogens in plants, with a focus on ornamental crops. Recent progress in molecular breeding, genetic engineering strategies, and RNAi technologies, such as HIGS and SIGS for the enhancement of fungal disease resistance in various important ornamental crops, is also described.

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“…In chrysanthemum, transfer of PGIP gene (polygalacturonase-inhibiting protein) from Prunus mumei reduced disease index and occurrence of Alternaria leaf spot (Miao et al, 2010 ). Similarly, chi II endorsed improved resistance to septoria leaf spot disease in transgenic chrysanthemum (Sen et al, 2013 ). De Cáceres González et al ( 2015 ) have produced transgenic L. longifolium cv.…”

Section: Abiotic and Biotic Stress Tolerancementioning

confidence: 99%

Biotechnological Advancements for Improving Floral Attributes in Ornamental Plants

et al. 2017

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Developing new ornamental cultivars with improved floral attributes is a major goal in floriculture. Biotechnological approach together with classical breeding methods has been used to modify floral color, appearance as well as for increasing disease resistance. Transgenic strategies possess immense potential to produce novel flower phenotypes that are not found in nature. Adoption of Genetic engineering has supported the idea of floral trait modification. Ornamental plant attributes like floral color, fragrance, disease resistance, and vase life can be improved by means of genetic manipulation. Therefore, we witness transgenic plant varieties of high aesthetic and commercial value. This review focuses on biotechnological advancements in manipulating key floral traits that contribute in development of diverse ornamental plant lines. Data clearly reveals that regulation of biosynthetic pathways related to characteristics like pigment production, flower morphology and fragrance is both possible and predictable. In spite of their great significance, small number of genetically engineered varieties of ornamental plants has been field tested. Today, novel flower colors production is regarded as chief commercial benefit obtained from transgenic plants. But certain other floral traits are much more important and have high commercial potential. Other than achievements such as novel architecture, modified flower color, etc., very few reports are available regarding successful transformation of other valuable horticultural characteristics. Our review also summarized biotechnological efforts related to enhancement of fragrance and induction of early flowering along with changes in floral anatomy and morphology.

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Agrobacterium Mediated Genetic Transformation of Chrysanthemum (Dendranthema grandiflora Tzvelev) with Rice Chitinase Gene for Improved Resistance Against Septoria obesa

Cited by 13 publications

References 18 publications

Towards the Improvement of Ornamental Attributes in Chrysanthemum: Recent Progress in Biotechnological Advances

Towards the Improvement of Ornamental Attributes in Chrysanthemum: Recent Progress in Biotechnological Advances

Recent Progress in Enhancing Fungal Disease Resistance in Ornamental Plants

Biotechnological Advancements for Improving Floral Attributes in Ornamental Plants

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