Agrobacterium-mediated horizontal gene transfer: Mechanism, biotechnological application, potential risk and forestalling strategy

Guo, Minliang; Ye, Jingyang; Gao, Dawei; Xu, Nan; Yang, Jing

doi:10.1016/j.biotechadv.2018.12.008

Cited by 79 publications

(47 citation statements)

References 143 publications

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“…This motile genetic element is responsible for tumor induction and biosynthesis of opines in plant tissue. It contains two regions associated with bacterial-plant interactions: vir regions, containing virulence genes (virA, virB, virC, virD, virE, virG, virF and virH) encoding proteins actively involved in the transfer and integration of the transgene with the plant genome, and a region containing genes coding the synthesis of inter alia opines, which are used as a source of carbon and nitrogen by bacteria [40]. This procedure allows the creation of transgenic plants by so called stable transformation, in which the new trait is inherited by further generations, or transient transformation, in which the genetic material persists in the cell nucleus without permanent integration with the genetic material.…”

Section: From Natural Gene Transfer To Plant Metabolic Engineeringmentioning

confidence: 99%

Transgenesis as a Tool for the Efficient Production of Selected Secondary Metabolites from Plant in Vitro Cultures

Kowalczyk

Wieczfińska

Skała

et al. 2020

Plants

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The plant kingdom abounds in countless species with potential medical uses. Many of them contain valuable secondary metabolites belonging to different classes and demonstrating anticancer, anti-inflammatory, antioxidant, antimicrobial or antidiabetic properties. Many of these metabolites, e.g., paclitaxel, vinblastine, betulinic acid, chlorogenic acid or ferrulic acid, have potential applications in medicine. Additionally, these compounds have many therapeutic and health-promoting properties. The growing demand for these plant secondary metabolites forces the use of new green biotechnology tools to create new, more productive in vitro transgenic plant cultures. These procedures have yielded many promising results, and transgenic cultures have been found to be safe, efficient and cost-effective sources of valuable secondary metabolites for medicine and industry. This review focuses on the use of various in vitro plant culture systems for the production of secondary metabolites.

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Section: From Natural Gene Transfer To Plant Metabolic Engineeringmentioning

confidence: 99%

Transgenesis as a Tool for the Efficient Production of Selected Secondary Metabolites from Plant in Vitro Cultures

Kowalczyk

Wieczfińska

Skała

et al. 2020

Plants

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“…The discovery of the various mechanisms of genetic exchange processes by which bacterial genomes recombine and the historical methods are interesting in themselves but also serve to introduce the diverse processes of recombination. In addition to the above well-recognized exchange mechanisms, there are other mechanisms also involved in genetic exchange mechanisms, and the extraordinary capacity of Agrobacterium in plants to transfer its genetic material to host cells makes it evolve from phytopathogen to a powerful transgenic vector [12].…”

Section: Mechanisms Of Hgtmentioning

confidence: 99%

Biosafety Issues of Unintended Horizontal Transfer of Recombinant DNA

Alamnie¹,

Andualem²

2020

Genetic Transformation in Crops

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Horizontal or lateral gene transfer is the transfer of genetic material from one organism to another organism associated with the unnatural way of reproduction. This way of genetic exchange happens by the stable transfer of genetic material from donor organisms, followed by heritable incorporation to the genome of the recipient organisms. The first explanation of a horizontal gene transfer (HGT) has been a major advance in molecular biology and genetics. Microorganisms like bacteria can acquire genetic material from a variety of sources. The most common DNA transfer mechanisms between microorganisms are conjugation, transformation, and transduction, but there are also other diverse mechanisms of genetic material exchange occurring in nature. Genetically modified organisms (GMOs) often contain recombined genes acquired from different species to enable the expression of new traits in even unlikely or unrelated species. For this, the unintended HGT of rDNA to both prokaryotes and eukaryotes could raise biosafety issues to both human and animal health and the environment. From the current scientific perspective and evidence, the unintended HGT from GMOs to other organisms presents negligible risks to human health and safety or the environment due to the rarity of such events relative to those HGT events that occur in nature.

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“…AMT is a complicated mechanism, which includes (1) signal recognition from plant host to A. tumefaciens, (Figure 2) [24]. First, bacterial chemotaxis attracted by phenolic compounds that are exuded from fresh wound site of plants, such as acetosyringone and α-hydroxy acetosyringone [25].…”

Section: The Natural Pathogenesis Of a Tumefaciensmentioning

confidence: 99%

Agrobacterium-Mediated Transformation

Pratiwi¹,

Surya²

2020

Genetic Transformation in Crops

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Agrobacterium-mediated transformation (AMT) heavily relies on the capability of bacterial pathogen Agrobacterium tumefaciens in transferring foreign genes into a wide variety of host plants. Currently, AMT is the most commonly used method for generating transgenic plants. On the other hand, A. tumefaciens was very useful for plant breeding. It also accelerated the technology of plant breeding to obtain specific characters. Gene transfer from bacteria to plants is a complex mechanism that involves several functional steps. This chapter will give brief information related to AMT mechanism, including the history of crown gall disease, the natural pathogenesis of A. tumefaciens, and the general protocol of AMT.

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Agrobacterium-mediated horizontal gene transfer: Mechanism, biotechnological application, potential risk and forestalling strategy

Cited by 79 publications

References 143 publications

Transgenesis as a Tool for the Efficient Production of Selected Secondary Metabolites from Plant in Vitro Cultures

Transgenesis as a Tool for the Efficient Production of Selected Secondary Metabolites from Plant in Vitro Cultures

Biosafety Issues of Unintended Horizontal Transfer of Recombinant DNA

Agrobacterium-Mediated Transformation

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