Agrobacterium-Mediated Transformation for the Development of Transgenic Crops; Present and Future Prospects

Rahman, Saleem Ur; Khan, Muhammad Omar; Ullah, Rahim; Ahmad, Fayaz; Raza, Ghulam

doi:10.1007/s12033-023-00826-8

Cited by 17 publications

(2 citation statements)

References 75 publications

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“…Protocols for regeneration of whole plants from poplar protoplasts have been described previously [314]. Potential limitations of agrotransformation also include the random integration of T-DNA into the plant genome, which can lead to either overexpression of the transgene or its silencing depending on the functional context of the chromatin; the sensitivity of plant lines to Agrobacterium strains can vary dramatically (some lines can be completely resistant, so their agrotransformation is impossible) and cannot be used to alter several genomic targets at once [315]. CRISPR/Cas-based genome editing technologies are being developed for plants, and poplar in particular, that can overcome some of the limitations of agrotransformation.…”

Section: Development Of Sustainable Geneticallymentioning

confidence: 99%

Editing Metabolism, Sex, and Microbiome: How Can We Help Poplar Resist Pathogens?

Kovalev,

Gladysh,

Bogdanova

et al. 2024

IJMS

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Poplar (Populus) is a genus of woody plants of great economic value. Due to the growing economic importance of poplar, there is a need to ensure its stable growth by increasing its resistance to pathogens. Genetic engineering can create organisms with improved traits faster than traditional methods, and with the development of CRISPR/Cas-based genome editing systems, scientists have a new highly effective tool for creating valuable genotypes. In this review, we summarize the latest research data on poplar diseases, the biology of their pathogens and how these plants resist pathogens. In the final section, we propose to plant male or mixed poplar populations; consider the genes of the MLO group, transcription factors of the WRKY and MYB families and defensive proteins BbChit1, LJAMP2, MsrA2 and PtDef as the most promising targets for genetic engineering; and also pay attention to the possibility of microbiome engineering.

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Section: Development Of Sustainable Geneticallymentioning

confidence: 99%

Editing Metabolism, Sex, and Microbiome: How Can We Help Poplar Resist Pathogens?

Kovalev,

Gladysh,

Bogdanova

et al. 2024

IJMS

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“…Compared to the conventional strategies used for functional characterization of plant genes and genetic improvement of agricultural crops, which are complicated and laborious-, time-consuming, CRISPR-Cas, especially CRISPR-Cas9 from Streptococcus pyogenes , has been proved in plant biology and provides more effective and time-saving methods with precise genetic manipulation of target genes ( Hsu et al., 2014 ; Liu et al., 2016 ). However, while CRISPR-Cas9 has enabled various genome editing applications, such as indels production, precise nucleotide substitution, gene-expression regulation, multiplexed and high-throughput gene editing in a variety of model plants and crop species, the recalcitrance of efficient genetic transformation severely hinders the application of CRISPR-Cas9 in the CWRs ( Rahman et al., 2023 ). Consequently, due to the lack of efficient CRISPR-Cas9 editing system, gene function verification of most CWRs in situ still cannot be carried out.…”

Section: Introductionmentioning

confidence: 99%

Establishment and application of Agrobacterium-delivered CRISPR/Cas9 system for wild tobacco (Nicotiana alata) genome editing

Yuan,

Zeng,

Liu

et al. 2024

Front. Plant Sci.

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Clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 (CRISPR-Cas9) system has been widely applied in cultivated crops, but limited in their wild relatives. Nicotiana alata is a typical wild species of genus Nicotiana that is globally distributed as a horticultural plant and well-studied as a self-incompatibility model. It also has valuable genes for disease resistance and ornamental traits. However, it lacks an efficient genetic transformation and genome editing system, which hampers its gene function and breeding research. In this study, we developed an optimized hypocotyl-mediated transformation method for CRISPR-Cas9 delivery. The genetic transformation efficiency was significantly improved from approximately 1% to over 80%. We also applied the CRISPR-Cas9 system to target the phytoene desaturase (NalaPDS) gene in N. alata and obtained edited plants with PDS mutations with over 50% editing efficiency. To generate self-compatible N. alata lines, a polycistronic tRNA-gRNA (PTG) strategy was used to target exonic regions of allelic S-RNase genes and generate targeted knockouts simultaneously. We demonstrated that our system is feasible, stable, and high-efficiency for N. alata genome editing. Our study provides a powerful tool for basic research and genetic improvement of N. alata and an example for other wild tobacco species.

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All Roads Lead to Rome: Pathways to Engineering Disease Resistance in Plants

Ikram,

Khan,

Islam

et al. 2024

Advanced Science

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Unlike animals, plants are unable to move and lack specialized immune cells and circulating antibodies. As a result, they are always threatened by a large number of microbial pathogens and harmful pests that can significantly reduce crop yield worldwide. Therefore, the development of new strategies to control them is essential to mitigate the increasing risk of crops lost to plant diseases. Recent developments in genetic engineering, including efficient gene manipulation and transformation methods, gene editing and synthetic biology, coupled with the understanding of microbial pathogenicity and plant immunity, both at molecular and genomic levels, have enhanced the capabilities to develop disease resistance in plants. This review comprehensively explains the fundamental mechanisms underlying the tug‐of‐war between pathogens and hosts, and provides a detailed overview of different strategies for developing disease resistance in plants. Additionally, it provides a summary of the potential genes that can be employed in resistance breeding for key crops to combat a wide range of potential pathogens and pests, including fungi, oomycetes, bacteria, viruses, nematodes, and insects. Furthermore, this review addresses the limitations associated with these strategies and their possible solutions. Finally, it discusses the future perspectives for producing plants with durable and broad‐spectrum disease resistance.

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Agrobacterium-Mediated Transformation for the Development of Transgenic Crops; Present and Future Prospects

Cited by 17 publications

References 75 publications

Editing Metabolism, Sex, and Microbiome: How Can We Help Poplar Resist Pathogens?

Editing Metabolism, Sex, and Microbiome: How Can We Help Poplar Resist Pathogens?

Establishment and application of Agrobacterium-delivered CRISPR/Cas9 system for wild tobacco (Nicotiana alata) genome editing

All Roads Lead to Rome: Pathways to Engineering Disease Resistance in Plants

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