Agrobacterium-mediated transformation of Dendrobium orchid with the flavanone 3-hydroxylase gene

Khumkarjorn, Nuntipa; Thanonkeo, Sudarat; Yamada, Mamoru; Klanrit, Poramate; Thanonkeo, Pornthap

doi:10.3906/bot-1701-13

Cited by 5 publications

(1 citation statement)

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“…Because of the limitations and inefficiencies of the traditional approaches, hybridization and mutagenesis can not be used to get some desirable traits, like the spotted blooms and foliage of a single plant. Agrobacterium-mediated transformation and particle bombardment methods have been routinely used in transgenic molecular breeding, leading to significant progress in horticultural development (Liau et al, 2003;Men et al, 2003;Hsing et al, 2016;Khumkarjorn et al, 2017;Lin et al, 2018;Kayika Febryanti et al, 2020;Setiawati et al, 2020). Our understanding of orchid reproductive biology will undoubtedly change as a result of these efforts to enhance orchid genomeediting tools and the power of large-scale genome sequencing, which will enable us to better understand the inherent roles of orchid genes and changes to genes of interest for desired blooming and floral features (Molla and Yang 2019;Nopitasari et al, 2020;Tong et al, 2020;Guo et al, 2022).…”

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confidence: 99%

In-depth analysis of genomes and functional genomics of orchid using cutting-edge high-throughput sequencing

et al. 2022

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High-throughput sequencing technology has been facilitated the development of new methodologies and approaches for studying the origin and evolution of plant genomes and subgenomes, population domestication, and functional genomics. Orchids have tens of thousands of members in nature. Many of them have promising application potential in the extension and conservation of the ecological chain, the horticultural use of ornamental blossoms, and the utilization of botanical medicines. However, a large-scale gene knockout mutant library and a sophisticated genetic transformation system are still lacking in the improvement of orchid germplasm resources. New gene editing tools, such as the favored CRISPR-Cas9 or some base editors, have not yet been widely applied in orchids. In addition to a large variety of orchid cultivars, the high-precision, high-throughput genome sequencing technology is also required for the mining of trait-related functional genes. Nowadays, the focus of orchid genomics research has been directed to the origin and classification of species, genome evolution and deletion, gene duplication and chromosomal polyploidy, and flower morphogenesis-related regulation. Here, the progressing achieved in orchid molecular biology and genomics over the past few decades have been discussed, including the evolution of genome size and polyploidization. The frequent incorporation of LTR retrotransposons play important role in the expansion and structural variation of the orchid genome. The large-scale gene duplication event of the nuclear genome generated plenty of recently tandem duplicated genes, which drove the evolution and functional divergency of new genes. The evolution and loss of the plastid genome, which mostly affected genes related to photosynthesis and autotrophy, demonstrated that orchids have experienced more separate transitions to heterotrophy than any other terrestrial plant. Moreover, large-scale resequencing provide useful SNP markers for constructing genetic maps, which will facilitate the breeding of novel orchid varieties. The significance of high-throughput sequencing and gene editing technologies in the identification and molecular breeding of the trait-related genes in orchids provides us with a representative trait-improving gene as well as some mechanisms worthy of further investigation. In addition, gene editing has promise for the improvement of orchid genetic transformation and the investigation of gene function. This knowledge may provide a scientific reference and theoretical basis for orchid genome studies.

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