Agrobacterium-Mediated Transformation of Plum (Prunus domestica L.) Hypocotyl Slices and Regeneration of Transgenic Plants

Mante, Seth; Morgens, Peter H.; Scorza, R.; Cordts, John; Callahan, Ann M.

doi:10.1038/nbt0991-853

Cited by 109 publications

(57 citation statements)

References 20 publications

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“…Pre-culturing of explants on co-cultivation medium rich in auxins prior to Agrobacterium inoculation has been shown to increase transformation efficiency in many woody plants such as plum (Mante et al, 1991), apricot (Laimer et al, 1992) and Arabidopsis (Sangwan et al, 1992) by increasing the number of competent cells at cut ends for transformation, but in case of citrus the reverse happened to be true as the transformation efficiency dropped to half after pre-culture treatment (Cervera et al,1998c;Costa et al, 2002).…”

Section: Agrobacterium-mediated Transformationmentioning

confidence: 99%

Citrus biotechnology: Achievements, limitations and future directions

Singh

Rajam

2009

Physiol Mol Biol Plants

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Citrus is one of the most important commercial and nutritional fruit crops in the world, hence it needs to be improved to cater to the diverse needs of consumers and crop breeders. Genetic manipulation through conventional techniques in this genus is invariably a difficult task for plant breeders as it poses various biological limitations comprising long juvenile period, high heterozygosity, sexual incompatibility, nucellar polyembryony and large plant size that greatly hinder cultivar improvement. Hence, several attempts were made to improve Citrus sps. by using various in vitro techniques. Citrus sps are widely known for their recalcitrance to transformation and subsequent rooting, but constant research has led to the establishment of improved protocols to ensure the production of uniformly transformed plants, albeit with relatively low efficiency, depending upon the genotype. Genetic modification through Agrobacterium-mediated transformation has emerged as an important tool for introducing agronomically important genes into Citrus sps. Somatic hybridization has been applied to overcome self and cross-incompatibility barriers and generated inter-specific and inter-generic hybrids. Encouraging results have been achieved through transgenics for resistance against viruses and bacteria, thereby augmenting the yield and quality of the fruit. Now, when major transformation and regeneration protocols have sufficiently been standardized for important cultivars, ongoing citrus research focuses mainly on incorporating such genes in citrus genotypes that can combat different biotic and abiotic stresses. This review summarizes the advances made so far in Citrus biotechnology, and suggests some future directions of research in this fruit crop.

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Section: Agrobacterium-mediated Transformationmentioning

confidence: 99%

Citrus biotechnology: Achievements, limitations and future directions

Singh

Rajam

2009

Physiol Mol Biol Plants

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“…Numerous studies have indicated that the effectiveness of transformation via different strains of Agrobacterium can be significantly different (e.g., De Bondt et al 1994;Le Gall et al 1994;Bond and Rose 1998;Cervera et al 1998;Gill et al 2004;Petri et al 2004;Joyce et al 2010). Several Agrobacterium strains, including LBA4404, EHA101, EHA105, GV3101, have been used in plum transformation previously (Mante et al 1991;Padilla et al 2003;Petri et al 2008). No transformation efficiency difference was found using EHA105 and LBA4404 (Padilla et al 2003).…”

Section: Fig 1 Transient Gus Expression In European Plum (Prunus Domentioning

confidence: 99%

“…For stable transformation studies, only hypocotyl slices were used in the experiments. Transformation was conducted using hypocotyls as explants as described by Mante et al (1991) and Padilla et al (2003) with modifications by Tian et al (2009) and in this study.…”

Section: Plant Materialsmentioning

confidence: 99%

“…European plum (Prunus domestica L.) is an economically important fruit crop and is widely grown across the world (Hartmann 1994;Okie and Ramming 1999;Kaufmane et al 2002). There have been a number of technology reports of European plum genetic transformation via Agrobacterium tumefaciens using hypocotyls as explants (Mante et al 1991;Padilla et al 2003;Petri et al 2008). These studies were actually all conducted in a single research laboratory.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of Factors Affecting European Plum (Prunus domestica L.) Genetic Transformation

Yuan¹,

Ali²,

Meerja³

et al. 2011

Genetic Transformation

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“…Commonly, the genetic transformation protocols of fruit species employed explants such as ovules, anthers, seedlings, zygotic and somatic embryos, cotyledons, epicotyles, hypocotyles, leaf pieces, roots, meristems (Fagoaga et al, 2007;Lopez-Perez et al, 2008;Petri et al, 2008;Husaini, 2010;Malnoy et al, 2010;Bosselut et al, 2011;Petri et al, 2011;Gago et al, 2011). Typically, it is recommended that those tissues have high and active cell division to enhance the regeneration of the transgenic lines (Mante et al, 1991;Schuerman & Dandekar, 1993;Wang, 2011). Ideally, fruit species transformation must be done with somatic tissues such as leaves and roots to transform varieties already well known and accepted in the market by the consumers.…”

Section: In Vitro Culture Techniques For the Recovery Of Transgenic Pmentioning

confidence: 99%