Agrobacterium–Mediated Transformation of Rice (Oryza sativa L.)

Raineri, Deanna M.; Bottino, P.J.; Gordon, Milton P.; Nester, Eugene W.

doi:10.1038/nbt0190-33

Cited by 160 publications

(69 citation statements)

References 38 publications

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“…High frequencies of resistant callus production and plantlet regeneration were obtained on G418-containig medium (Tab 2). This conclusion is in contrast to that of Raineri [16], Chan [17] and He [18]. In our study, the high efficiency of obtaining transgenic plants was obtained in immature embryos infected with EHA101/pExT 7 preinduced with AS followed by cocultivation in the presence of AS.…”

Section: Co-cultivation Selection and Plant Regenerationcontrasting

confidence: 51%

Agrobacterium tumefaciens-mediated transformation of rice with the spider insecticidal gene conferring resistance to leaffolder and striped stem borer

HUANG

Wei

et al. 2001

Cell Res

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Immature embryos of rice varieties Xiushui11 and Chunjiang 11 precultured for 4d were infected and transformed by Agrobacterium tumefaciens strain EHA101/pExT 7 (containing the spider insecticidal gene). The resistant calli were transferred onto the differentiation medium and plants were regenerated. The transformation frequency reached 56% ~ 72% measured as numbers of Geneticin (G418)-resistant calli produced and 36% ~ 60% measured as numbers of transgenic plants regenerated, respectively. PCR and Southern blot analysis of transgenic plants confirmed that the T-DNA had been integrated into the rice genome. Insect bioassays using T1 transgenic plants indicated that the mortality of the leaffolder (Cnaphalocrasis medinalis) after 7d of leaf feeding reached 38% ~ 61% and the corrected mortality of the striped stem borer (Chilo suppressalis) after 7d of leaf feeding reached 16% ~ 75%. The insect bioassay results demonstrated that the transgenic plants expressing the spider insecticidal protein conferred enhanced resistance to these pests.

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Section: Co-cultivation Selection and Plant Regenerationcontrasting

confidence: 51%

Agrobacterium tumefaciens-mediated transformation of rice with the spider insecticidal gene conferring resistance to leaffolder and striped stem borer

HUANG

Wei

et al. 2001

Cell Res

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“…Severa1 reports presented early attempts to transform the Gramineae with A. tumefaciens, including A. tumefaciensmediated infection of plants with vira1 genomes (Grimsley et al, 1988;Raineri et al, 1990;Gould et al, 1991;Mooney et al, 1991;Chan et al, 1992Chan et al, , 1993Schlappi and Hohn, 1992;Shen et al, 1993). Chan et al (1993) first reported the production of transgenic rice plants by inoculating immature embryos with an A. tumefaciens strain and proved the transformation by molecular and genetic analysis.…”

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confidence: 99%

Genetic Transformation of Wheat Mediated by Agrobacterium tumefaciens

et al. 1997

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A rapid Agrobacferium fumefaciens-mediated transformation system for wheat was developed using freshly isolated immature embryos, precultured immature embryos, and embryogenic calli as explants. l h e explants were inoculated with a disarmed A. tumefaciens strain C58 (ABI) harboring the binary vector pMON18365 containing the p-glucuronidase gene with an intron, and a selectable marker, the neomycin phosphotransferase II gene. Various factors were found to influence the transfer-DNA delivery efficiency, such as explant tissue and surfactants present in the inoculation medium. The inoculated immature embryos or embryogenic calli were selected on G418-containing media. Transgenic plants were regenerated from all three types of explants. The total time required from inoculation to the establishment of plants i n soil was

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“…However, native rice callus shows various degrees of in-built natural resistance to Kanamycin (Caplan et al 1992). Raineri et al (1990) used 200 mg l −1 Kanamycin to select rice callus derived from mature embryos, but no transgenic plants were recovered. Selection on G418 is more effective than Kanamycin for transgenic plants expressing nptII gene, perhaps because G418 is more toxic than Kanamycin to rice cells (Twyman et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Agrobacterium-mediated genetic transformation of commercially elite rice restorer line using nptII gene as a plant selection marker

Chakraborty

Reddy

Narasu

et al. 2016

Physiol Mol Biol Plants

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Transformation of commercially important indica cultivars remains challenging for the scientific community even though Agrobacterium-mediated transformation protocols for a few indica rice lines have been well established. We report successful transformation of a commercially important restorer line JK1044R of indica rice hybrid JKRH 401. While following existing protocol, we optimized several parameters for callusing, regeneration and genetic transformation of JK1044R. Calli generated from the rice scutellum tissue were used for transformation by Agrobacterium harboring pCAMBIA2201. A novel two tire selection scheme comprising of Geneticin (G418) and Paramomycin were deployed for selection of transgenic calli as well as regenerated plantlets that expressed neomycin phosphotransferase-II gene encoded by the vector. One specific combination of G418 (30 mg l −1 ) and Paramomycin (70 mg l −1 ) was very effective for calli selection. Transformed and selected calli were detected by monitoring the expression of the reporter gene uidA (GUS). Regenerated plantlets were confirmed through PCR analysis of nptII and gus genes specific primers as well as dot blot using gus gene specific as probe.

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Agrobacterium–Mediated Transformation of Rice (Oryza sativa L.)

Cited by 160 publications

References 38 publications

Agrobacterium tumefaciens-mediated transformation of rice with the spider insecticidal gene conferring resistance to leaffolder and striped stem borer

Agrobacterium tumefaciens-mediated transformation of rice with the spider insecticidal gene conferring resistance to leaffolder and striped stem borer

Genetic Transformation of Wheat Mediated by Agrobacterium tumefaciens

Agrobacterium-mediated genetic transformation of commercially elite rice restorer line using nptII gene as a plant selection marker

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