Agrobacterium-mediated transformation systems of Primula vulgaris

Hayta, Şadiye; Smedley, Mark; Li, Jinhong; Harwood, Wendy; Gilmartin, Philip M.

doi:10.1186/s13007-018-0360-1

Cited by 21 publications

(8 citation statements)

References 47 publications

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“…Thus, our ndings suggest to maintain explants for additional 20-30 days on antibiotic selection for an augmented positive rate. This idea is consistent with the previous works (Hayta et al 2018;Larkin et al 1996).…”

Section: Comparison Of Two Transformationsupporting

confidence: 94%

Two Agrobacterium-mediated Transformation Protocols of White Clover (Trifolium Repens) Through the Callus System

Jia

Cheng

Feng

et al. 2022

Preprint

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White clover (Trifolium repens) is one of the most widely cultivated livestock forage legumes co-cultivated worldwide with pasture grass in a mixed-sward setting, however, its persistence and aesthetic quality are severely affected by abiotic stresses. In this study, regeneration of white clover plants was conducted through a callus system for 4-5 months with a regeneration frequency of 36-41%. Inoculating 4-day-old cotyledons into MS media fortified with 0.4 mg·L-1 6-BA and 2 mg·L-1 2,4-D significantly increased the callus formation rate. Roots and cotyledons were better induced, followed by hypocotyls, leaves, and petioles. The development of differentiated structures performed effectively on MS supplemented with 1 mg·L-1 6-BA and 0.1 mg·L-1 NAA. Further, we determined factors affecting the Agrobacterium tumefaciens-mediated transient transformation for root-derived callus and 4-day-old cotyledons. The parameters that facilitated transient transformation were: Agrobacterium suspension density of 0.5 (OD600), 20 mg·L-1 AS, and 4-days co-cultivation duration. Subsequently, we developed two transformation protocols: transformation after callus formation in root segments (Protocol A) and transformation before callus initiation in 4-day-old cotyledons (Protocol B). The transformation frequencies varied from 1.92% to 3.17% in Protocol A and from 2.76% to 3.47% in Protocol B. We offer the possibility to regenerate multiple transgenic white clover from a single genetic background. In addition to assistance in identification of functional genes associated with yield, resistance and aesthetic quality, our research will also contribute to successful genetic manipulation and genome editing in white clover.

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Section: Comparison Of Two Transformationsupporting

confidence: 94%

Two Agrobacterium-mediated Transformation Protocols of White Clover (Trifolium Repens) Through the Callus System

Jia

Cheng

Feng

et al. 2022

Preprint

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“…Furthermore, transient expression systems of different plant species have been gradually established. For instance, Primula vulgaris ( Hayta et al, 2018 ), Panicum virgatum ( Chen et al, 2010 ), Artemisia annua ( Li et al, 2021 ), and Citrus reticulata ( Gong et al, 2021 ). Compared with these plants, N .…”

Section: Resultsmentioning

confidence: 99%

Engineering the expression of plant secondary metabolites-genistein and scutellarin through an efficient transient production platform in Nicotiana benthamiana L.

et al. 2022

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Plant natural products (PNPs) are active substances indispensable to human health with a wide range of medical and commercial applications. However, excessive population growth, overexploitation of natural resources, and expensive total chemical synthesis have led to recurrent supply shortages. Despite the fact that the microbial production platform solved these challenges, the platform still has drawbacks such as environmental pollution, high costs, and non-green production. In this study, an efficient platform for the production of PNPs based on the transient expression system of Nicotiana benthamiana L. combined with synthetic biology strategies was developed. Subsequently, the feasibility of the platform was verified by a simple “test unit.” This platform was used to synthesize two high-value PNPs: genistein (5.51 nmol g–1 FW) and scutellarin (11.35 nmol g–1 FW). Importantly, this is the first report on the synthesis of scutellarin in heterologous plants. The platform presented here will possibly be adopted for the heterologous production of genistein and scutellarin in tobacco plants as a novel and sustainable production strategy.

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“…The recombinant plasmid verified by PCR and sequencing was named pCPB121-StCIPK18. The constructed recombinant plasmid was transferred to Agrobacterium GV3101(Angyubio, Shanghai, China) [ 38 ].…”

Section: Methodsmentioning

confidence: 99%

CBL-Interacting Protein Kinases 18 (CIPK18) Gene Positively Regulates Drought Resistance in Potato

Yang

Zhang

Wang

et al. 2023

IJMS

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Sensor—responder complexes comprising calcineurin B-like (CBL) proteins and CBL-interacting protein kinases (CIPKs) are plant-specific Ca2+ receptors, and the CBL-CIPK module is widely involved in plant growth and development and a large number of abiotic stress response signaling pathways. In this study, the potato cv. “Atlantic” was subjected to a water deficiency treatment and the expression of StCIPK18 gene was detected by qRT-PCR. The subcellular localization of StCIPK18 protein was observed by a confocal laser scanning microscope. The StCIPK18 interacting protein was identified and verified by yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC). StCIPK18 overexpression and StCIPK18 knockout plants were constructed. The phenotypic changes under drought stress were indicated by water loss rate, relative water content, MDA and proline contents, and CAT, SOD and POD activities. The results showed that StCIPK18 expression was upregulated under drought stress. StCIPK18 is localized in the cell membrane and cytoplasm. Y2H shows the interaction between StCIPK18 and StCBL1, StCBL4, StCBL6 and StCBL8. BiFC further verifies the reliability of the interaction between StCIPK18 and StCBL4. Under drought stress, StCIPK18 overexpression decreased the water loss rate and MDA, and increased RWC, proline contents and CAT, SOD and POD activities; however, StCIPK18 knockout showed opposite results, compared with the wild type, in response to drought stress. The results can provide information for the molecular mechanism of the StCIPK18 regulating potato response to drought stress.

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Agrobacterium-mediated transformation systems of Primula vulgaris

Cited by 21 publications

References 47 publications

Two Agrobacterium-mediated Transformation Protocols of White Clover (Trifolium Repens) Through the Callus System

Two Agrobacterium-mediated Transformation Protocols of White Clover (Trifolium Repens) Through the Callus System

Engineering the expression of plant secondary metabolites-genistein and scutellarin through an efficient transient production platform in Nicotiana benthamiana L.

CBL-Interacting Protein Kinases 18 (CIPK18) Gene Positively Regulates Drought Resistance in Potato

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