Agrobacterium rhizogenes mediated transformation of the medicinal plant Decalepis arayalpathra and production of 2-hydroxy-4-methoxy benzaldehyde

Sudha, C. G.; Sherina, T. V.; Anand, V. P. Anu; Reji, J. V.; Padmesh, P.; Soniya, E. V.

doi:10.1007/s11240-012-0226-6

Cited by 34 publications

(11 citation statements)

References 29 publications

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“…The T R -DNA, which only exists in agropine-type plasmids of A. rhizogenes and controls opine and auxin biosynthesis, is responsible for tumors (Veena and Taylor 2007). Sudha et al (2012) indicated that tumors might be induced by the effects of endogenous hormones accompanied by a bacterial strain in response to the rol gene products.…”

Section: Resultsmentioning

confidence: 99%

Hairy root induction and plant regeneration of medicinal plant Dracocephalum kotschyi

Sharafi

Sohi

Azadi

et al. 2014

Physiol Mol Biol Plants

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An efficient hairy root induction system for an important endangered medicinal plant, Dracocephalum kotschyi, was developed through Agrobacterium rhizogenesmediated transformation by modifying the co-cultivation medium using five bacterial strains, A4, ATCC15834, LBA9402, MSU440, and A13 (MAFF-02-10266). A drastic increase in transformation frequency was observed when a Murashige and Skoog medium lacking NH 4 NO 3 KH 2 PO 4, KNO 3 and CaCl 2 was used, resulting in hairy root induction frequencies of 52.3 %, 69.6 %, 48.6 %, 89.0 %, and 80.0 % by A4, A13, LBA9402, MSU440, and ATCC15834 strains, respectively. For shoot induction, hairy roots and unorganized tumors induced by strain ATCC15834 were placed on an MS media supplemented with 0.1, 0.25, 0.5, and 1 mg/l BA plus 0.1 mg/l NAA. The high frequency of shoot regeneration and number of shoot were obtained in the medium containing 0.25 mg/l BA and 0.1 mg/l NAA. Root induction occurred from the base of regenerated shoots on the MS medium supplemented with 0.5 mg/l IBA after 10 days.

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Section: Resultsmentioning

confidence: 99%

Hairy root induction and plant regeneration of medicinal plant Dracocephalum kotschyi

Sharafi

Sohi

Azadi

et al. 2014

Physiol Mol Biol Plants

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“…All of the A. rhizogenes strains led to hairy root induction, but MSU440 and ATCC15834 were significantly effective than A4 and A13. It is revealed that the ability of A. rhizogenes to plant transformation is strain dependent (Sudha et al 2012). …”

Section: Hairy Roots Inductionmentioning

confidence: 99%

In vitro regeneration and Agrobacterium mediated genetic transformation of Artemisia aucheri Boiss

Sharafi

Sohi

Mirzaee

et al. 2014

Physiol Mol Biol Plants

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In the present study, we developed an efficient protocol for in vitro plant regeneration and genetically transformed root induction in medicinal plant Artemisia aucheri Boiss. Leaf explants were cultivated in MS medium supplemented by combination of plant growth regulators including α-naphthalene-acetic acid, 6-benzyl-aminopurine, indole-3-acetic acid and 2, 4-dichlorophenoxyaceticacid. The highest frequency of shoot organogenesis occurred on MS medium supplemented with 0.05 mg/l NAA plus 2 mg/l BA (96.3 %) and MS medium supplemented with 0.5 mg/l IAA plus 2 mg/l BA (88.3 %). Root induction was obtained on MS medium supplemented with 0.5 mg/l IBA. This is a simple, reliable, rapid and high efficient regeneration system for A. aucheri Boiss in short period via adventitious shoot induction approach. Also, an efficient genetically transformed root induction for A. aucheri was developed through Agrobacterium rhizogenes-mediated transformation by four bacterial strains, A4, ATCC15834, MSU440, and A13 (MAFF-02-10266). The maximum frequency of hairy root induction was obtained using MSU440 (93 %) and ATCC15834 (89 %) bacterial strains. Hairy root lines were confirmed by PCR using the rolB gene specific primers and Southern blot analysis.

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“…Tumorigenic calli formed at the infection spot of explants might be because of T R -DNA transferring iaaM and iaaH genes, which are accountable for auxins biosynthesis (23). This might be prompted by the special effects of endogenous hormone combined with the interface between explant and bacterial strain in reaction to the rol gene products (35). As tumorigenic calli is not appropriate for large scale productions, the results indicated that strain MSU440, inducing high frequency of genetically transformed roots and low amount tumorigenic calli, is more capable than the other bacterial strains used in this study.…”

Section: Discussionmentioning

confidence: 99%

Genetically Transformed Root-Based Culture Technology in Medicinal Plant Cosmos bipinnatus

Jaberi¹,

Sharafi²,

Sharafi³

et al. 2018

Jundishapur J Nat Pharm Prod

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Background: Cosmos bipinnatus is an important medicinal plant with antioxidative, antigenotoxic, anti-inflammatory, and antiproliferative effects on several cancer cell lines. It has great potential for development as a promising cancer chemo-preventive agent. Hairy root-based culture technology is a new sustainable production platform for producing specific pharmaceutical secondary metabolites. Objectives: The current study developed and introduced a reliable transformation system for C. bipinnatus by optimization of aspects important in transformation frequency using Agrobacterium rhizogenes. Methods: Five bacterial strains, including ATCC 15834, ATCC 31798, A7, MAFF-02-10266, and MSU440, 2 explant types (leaf and stem), and 2 co-cultivation media (full MS and ½ MS) were examined. Genomic DNA was extracted using a modified CTAB protocol from putative transgenic root lines and the control root. Transgenic hairy root lines were approved by means of Polymerase Chain Reaction (PCR) using specific rolB gene primers. Results:The highest ratio of genetically transformed root induction was found from leaf explants using A. rhisogenes strains ATCC15834 and MSU440 (72% to 73%). When ½ MS medium was used as a co-cultivation medium, a significant increase in transformation frequency (84%) was observed. Conclusions: The MSU440 Agrobacterium strain and ½ MS co-cultivation medium could significantly improve genetic transformation efficiency for establishment of hairy root-based cultures for C. bipinnatus.

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Agrobacterium rhizogenes mediated transformation of the medicinal plant Decalepis arayalpathra and production of 2-hydroxy-4-methoxy benzaldehyde

Cited by 34 publications

References 29 publications

Hairy root induction and plant regeneration of medicinal plant Dracocephalum kotschyi

Hairy root induction and plant regeneration of medicinal plant Dracocephalum kotschyi

In vitro regeneration and Agrobacterium mediated genetic transformation of Artemisia aucheri Boiss

Genetically Transformed Root-Based Culture Technology in Medicinal Plant Cosmos bipinnatus

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