Agrobacterium tumefaciens–mediated genetic transformation and overexpression of the flavonoid 3′5′-hydroxylase gene increases the flavonoid content of the transgenic Aconitum carmichaelii Debx. plant

Nguyen, Tan N.; Hoang, Thi Thu Hoan; Nguyen, Huu Quan; Tân, Từ Quang; Tran, Thi Hong; Lo, Thi Mai Thu; Vu, Thi Thu Thuy; Mậu, Chu Hoàng

doi:10.1007/s11627-021-10190-4

Cited by 11 publications

(5 citation statements)

References 39 publications

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“…This information not only demonstrated the involvement of CpF3′5′H_2 in the response of the wild relative to herbivory but also corroborated with earlier studies from our group towards understanding the chemical basis of resistance response in C. platycarpus [ 11 ]. Though there have been studies demonstrating the utility of increased expression of F3′5′H in plants [ 21 , 24 , 25 ], deciphering the role of genes identified during the resistance response towards herbivory has been scarce.…”

Section: Resultsmentioning

confidence: 99%

Cajanus platycarpus Flavonoid 3′5′ Hydroxylase_2 (CpF3′5′H_2) Confers Resistance to Helicoverpa armigera by Modulating Total Polyphenols and Flavonoids in Transgenic Tobacco

Tyagi

Rathinam

Dokka

et al. 2023

IJMS

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Pod borer Helicoverpa armigera, a polyphagus herbivorous pest, tremendously incurs crop damage in economically important crops. This necessitates the identification and utility of novel genes for the control of the herbivore. The present study deals with the characterization of a flavonoid 3′5′ hydroxylase_2 (F3′5′H_2) from a pigeonpea wild relative Cajanus platycarpus, possessing a robust chemical resistance response to H. armigera. Though F3′5′H_2 displayed a dynamic expression pattern in both C. platycarpus (Cp) and the cultivated pigeonpea, Cajanus cajan (Cc) during continued herbivory, CpF3′5′H_2 showed a 4.6-fold increase vis a vis 3-fold in CcF3′5′H_2. Despite similar gene copy numbers in the two Cajanus spp., interesting genic and promoter sequence changes highlighted the stress responsiveness of CpF3′5′H_2. The relevance of CpF3′5′H_2 in H. armigera resistance was further validated in CpF3′5′H_2-overexpressed transgenic tobacco based on reduced leaf damage and increased larval mortality through an in vitro bioassay. As exciting maiden clues, CpF3′5′H_2 deterred herbivory in transgenic tobacco by increasing total flavonoids, polyphenols and reactive oxygen species (ROS) scavenging capacity. To the best of our knowledge, this is a maiden attempt ascertaining the role of F3′5′H_2 gene in the management of H. armigera. These interesting leads suggest the potential of this pivotal branch-point gene in biotic stress management programs.

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Section: Resultsmentioning

confidence: 99%

Cajanus platycarpus Flavonoid 3′5′ Hydroxylase_2 (CpF3′5′H_2) Confers Resistance to Helicoverpa armigera by Modulating Total Polyphenols and Flavonoids in Transgenic Tobacco

Tyagi

Rathinam

Dokka

et al. 2023

IJMS

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“…For example, cloning F3′5′H genes can change the color of plants, such as roses and carnations that do not naturally have F3′5′H activities, from lavender to blue [23][24][25]. [20,21,46]. In these studies, blueberry genes were identified with bioinformatics analyses.…”

Section: Analysis Of the Basic Characteristics Of Blueberry F3′5′hmentioning

confidence: 99%

“…F3′5′H was first cloned from petunia and was found to be involved in pigment synthesis [14,15]. Its homologs were subsequently cloned from various plants, including gentian [16], Arabidopsis [17], Solanum tuberosum [18], tomato [19], Ginkgo [20], and Aconitum [21]. F3′5′H genes are linked to anthocyanin synthesis.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Analysis of the F3′5′H Gene Family in Blueberry (Vaccinium corymbosum L.) Provides Insights into the Regulation of Anthocyanin Biosynthesis

Guo¹,

Hu²,

Yang³

et al. 2023

Phyton

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The F3′5′H gene family plays an important role in regulating anthocyanin biosynthesis, abiotic stress, and hormone signaling. In this study, 14 F3′5′H genes were identified from the blueberry genome. The chromosomal distribution, physicochemical properties, F3′5′H domain, conserved motifs, cis-acting elements, and intron/exon compositions were analyzed. The functional prediction analysis of these VcF3′5′Hs indicated that their biological functions included light response and other secondary metabolites. The results of qRT-PCR showed that VcF3′5′Hs (especially VcF3′5′H4) were highly expressed at the ripening stage. Subcellular localization revealed that VcF3′5′H4 may be located in the endoplasmic reticulum. Co-expression analysis showed that the VcF3′5′H gene family was related to anthocyanin. This research provides an overview of the blueberry F3′5′H family and helps verify the role of these genes in regulating anthocyanin biosynthesis.

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“…As a result, the F3 5 H gene is often referred to as the "blue gene" [12,21,22], and it has attracted considerable attention as a crucial molecular tool for generating blue flower colors through genetic engineering techniques [6]. Since the first F3 5 H was isolated from Petunia hybrida [23], to date, the homologous genes of F3 5 H have been isolated from many plants, including C. medium [24], Vinca major [25], Verbena hybrida [26], Phalaenopsis [27,28], Glycine soja [29], Cyclamen persicum [30], Dendrobium moniliforme [31], Antirrhinum kelloggii [8], Epimedium sagittatum [32], Pericallis hybrida [22], Camellia sinensis [12], and Aconitum carmichaelii [33,34]. By heterologous over-expression, some F3 5 Hs have been used to increase the accumulation of delphinidin-based anthocyanins for producing a blue color in some flower crops, such as roses [1,2,6,18], carnations [2,6,17,18] and chrysanthemums [7,14].…”

Section: Introductionmentioning

confidence: 99%

Heterologous Expression of Platycodon grandiflorus PgF3′5′H Modifies Flower Color Pigmentation in Tobacco

Ma,

Jia,

Duan

et al. 2023

Genes

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Flavonoid-3′,5′-hydroxylase (F3′5′H) is the key enzyme for the biosynthesis of delphinidin-based anthocyanins, which are generally required for purple or blue flowers. Previously, we isolated a full-length cDNA of PgF3′5′H from Platycodon grandiflorus, which shared the highest homology with Campanula medium F3′5′H. In this study, PgF3′5′H was subcloned into a plant over-expression vector and transformed into tobacco via Agrobacterium tumefaciens to investigate its catalytic function. Positive transgenic tobacco T0 plants were obtained by hygromycin resistance screening and PCR detection. PgF3′5′H showed a higher expression level in all PgF3′5′H transgenic tobacco plants than in control plants. Under the drive of the cauliflower mosaic virus (CaMV) 35S promoter, the over-expressed PgF3′5′H produced dihydromyricetin (DHM) and some new anthocyanin pigments (including delphinidin, petunidin, peonidin, and malvidin derivatives), and increased dihydrokaempferol (DHK), taxifolin, tridactyl, cyanidin derivatives, and pelargonidin derivatives in PgF3′5′H transgenic tobacco plants by ultra-performance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS) analysis, resulting in a dramatic color alteration from light pink to magenta. These results indicate that PgF3′5′H products have F3′5′H enzyme activity. In addition, PgF3′5′H transfer alters flavonoid pigment synthesis and accumulation in tobacco. Thus, PgF3′5′H may be considered a candidate gene for gene engineering to enhance anthocyanin accumulation and the molecular breeding project for blue flowers.

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Agrobacterium tumefaciens–mediated genetic transformation and overexpression of the flavonoid 3′5′-hydroxylase gene increases the flavonoid content of the transgenic Aconitum carmichaelii Debx. plant

Cited by 11 publications

References 39 publications

Cajanus platycarpus Flavonoid 3′5′ Hydroxylase_2 (CpF3′5′H_2) Confers Resistance to Helicoverpa armigera by Modulating Total Polyphenols and Flavonoids in Transgenic Tobacco

Cajanus platycarpus Flavonoid 3′5′ Hydroxylase_2 (CpF3′5′H_2) Confers Resistance to Helicoverpa armigera by Modulating Total Polyphenols and Flavonoids in Transgenic Tobacco

Genome-Wide Analysis of the F3′5′H Gene Family in Blueberry (Vaccinium corymbosum L.) Provides Insights into the Regulation of Anthocyanin Biosynthesis

Heterologous Expression of Platycodon grandiflorus PgF3′5′H Modifies Flower Color Pigmentation in Tobacco

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