Agrobacterium tumefaciens-mediated transformation of common bean (Phaseolus vulgaris) var. Brunca

Solís-Ramos, Laura Y.; Ortiz-Pavón, Julio-César; Andrade‐Torres, Antonio; Porras-Murillo, Romano; Brenes-Angulo, Arturo; Serna, Enrique Castaño de la

doi:10.15517/rbt.v67i2supl.37208

Cited by 8 publications

(5 citation statements)

References 24 publications

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“…The transformation frequency is a cultivar‐dependent trait (Mukeshimana et al, 2013 ). Agrobacterium‐mediated transformation and shoot regeneration through somatic embryogenesis has been successfully implemented recently but with low transformation frequencies (0.5%–2.5%) indicating that this technology is far from routine (Solís‐Ramos et al, 2019 ; Song et al, 2020 ).…”

Section: Genetic Resources and Biofortification Strategies For Common...mentioning

confidence: 99%

Biofortification of common bean (Phaseolus vulgaris L.) with iron and zinc: Achievements and challenges

Huertas

Karpińska

Ngala

et al. 2022

Food and Energy Security

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Micronutrient deficiencies (hidden hunger), particularly in iron (Fe) and zinc (Zn), remain one of the most serious public health challenges, affecting more than three billion people globally. A number of strategies are used to ameliorate the problem of micronutrient deficiencies and to improve the nutritional profile of food products. These include (i) dietary diversification, (ii) industrial food fortification and supplements, (iii) agronomic approaches including soil mineral fertilisation, bioinoculants and crop rotations, and (iv) biofortification through the implementation of biotechnology including gene editing and plant breeding.These efforts must consider the dietary patterns and culinary preferences of the consumer and stakeholder acceptance of new biofortified varieties. Deficiencies in Zn and Fe are often linked to the poor nutritional status of agricultural soils, resulting in low amounts and/or poor availability of these nutrients in staple food crops such as common bean. This review describes the genes and processes associated with Fe and Zn accumulation in common bean, a significant food source in Africa that plays an important role in nutritional security. We discuss the conventional plant breeding, transgenic and gene editing approaches that are being deployed to improve Fe and Zn accumulation in beans. We also consider the requirements of successful bean biofortification programmes, highlighting gaps in current knowledge, possible solutions and future perspectives.

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Section: Genetic Resources and Biofortification Strategies For Common...mentioning

confidence: 99%

Biofortification of common bean (Phaseolus vulgaris L.) with iron and zinc: Achievements and challenges

Huertas

Karpińska

Ngala

et al. 2022

Food and Energy Security

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“…Brunca, periodos de co-cultivo de tres a cuatro días, resultaron en una integración adecuada de los genes GUS o nptII (Solís-Ramos et al, 2019). En la presente investigación se demostró que para el cultivar 'ICA Pijao', tres días de co-cultivo reducen las afectaciones en la regeneración de brotes en un 10 %.…”

Section: Tiempo De Infiltración Por Vacíounclassified

Regeneración de brotes a partir de tejido transformado en (Phaseolus vulgaris) Fabaceae cultivares 'ICA Pijao' y 'BAT 93'

et al. 2022

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El cultivo de Phaseolus vulgaris L. se encuentra muy difundido en el mundo. La transformación genética vía Agrobacterium tumefaciens constituye una alternativa en esta especie para la obtención de cultivares con tolerancia o resistencia a condiciones ambientales desfavorables. Sin embargo, la condición cultivar dependiente y la baja regeneración del tejido transformado en los protocolos establecidos para P. vulgaris, han limitado el empleo de esta tecnología a un grupo reducido de cultivares. El objetivo de este trabajo fue regenerar brotes a partir de tejido transformado en P. vulgaris cv. 'ICA Pijao’ y 'BAT 93’. Se determinó el efecto de la concentración de la suspensión bacteriana, la infiltración por vacío, el periodo de co-cultivo sobre la regeneración de brotes. Además, se determinó la efectividad de la geneticina y el glufosinato de amonio durante la etapa de selección en la regeneración de brotes a partir de explantes inoculados con A. tumefaciens. Los explantes ½ NC-1 inoculados con una suspensión bacteriana ajustada a una densidad óptica de 0,1, sin infiltración con vacío y mantenidos por tres días en co-cultivo presentaron la mejor respuesta de regeneración. La geneticina y el glufosinato de amonio fueron efectivos para la selección de brotes. La regeneración de brotes a partir de tejido transformado vía A. tumefaciens en P. vulgaris cv. 'ICA Pijao' y 'BAT 93', no descrita en la literatura científica, resulta importante para su posterior aplicación en programas de mejoramiento genéticos de esta especie.

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“…The preliminary results laid the foundation of this research. Because recalcitrance of common bean genotypes to in vitro regeneration from non-meristem containing tissues is the primary limitation for stable transformation [16,25,26], the preculture method described for the Olathe' pinto bean may facilitate transformation of the other common bean cultivars. In fact, it has been reported that the EHA105-mediated transformation of primary and proliferative calli of common bean cv.…”

Section: Meristem Tissues Are a Reliable Source Of Regenerable Callimentioning

confidence: 99%

“…An efficient and reproducible transformation system for the production of stable transgenic common bean plants is still lacking. The primary limitation has been recalcitrance of common bean genotypes to in vitro regeneration from non-meristem containing tissues [16,25,26].…”

Section: Introductionmentioning

confidence: 99%

“…Agrobacterium tumefaciens-mediated transformation is desirable for common bean transformation because of its accessibility and tendency to produce low-or single-copy insertion(s) of the transgene [18]. To date, A. tumefaciens-mediated transformation of common bean has been studied extensively but has been met with limited success [16,25]. We have been working on common bean regeneration and transformation since 2008.…”

Section: Introductionmentioning

confidence: 99%

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Induction of competent cells for Agrobacterium tumefaciens-mediated stable transformation of common bean (Phaseolus vulgaris L.)

et al. 2020

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Stable transformation of common bean (Phaseolus vulgaris L.) has been successful, to date, only using biolistic-mediated transformation and shoot regeneration from meristemcontaining embryo axes. In this study, using precultured embryo axes, and optimal co-cultivation conditions resulted in a successful transformation of the common bean cultivar Olathe using Agrobacterium tumefaciens strain EHA105. Plant regeneration through somatic embryogenesis was attained through the preculture of embryo axes for 12 weeks using induced competent cells for A. tumefaciens-mediated gene delivery. Using A. tumefaciens at a low optical density (OD) of 0.1 at a wavelength of 600 nm for infection and 4-day co-cultivation, compared to OD 600 of 0.5, increased the survival rate of the inoculated explants from 23% to 45%. Selection using 0.5 mg L-1 glufosinate (GS) was effective to identify transformed cells when the bialaphos resistance (bar) gene under the constitutive 35S promoter was used as a selectable marker. After an 18-week selection period, 1.5%-2.5% inoculated explants, in three experiments with a total of 600 explants, produced GS-resistant plants through somatic embryogenesis. The expression of bar was confirmed in first-and secondgeneration seedlings of the two lines through reverse polymerase chain reaction. Presence of the bar gene was verified through genome sequencing of two selected transgenic lines. The induction of regenerable, competent cells is key for the successful transformation, and the protocols described may be useful for future transformation of additional Phaseolus germplasm.

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Agrobacterium tumefaciens-mediated transformation of common bean (Phaseolus vulgaris) var. Brunca

Cited by 8 publications

References 24 publications

Biofortification of common bean (Phaseolus vulgaris L.) with iron and zinc: Achievements and challenges

Biofortification of common bean (Phaseolus vulgaris L.) with iron and zinc: Achievements and challenges

Regeneración de brotes a partir de tejido transformado en (Phaseolus vulgaris) Fabaceae cultivares 'ICA Pijao' y 'BAT 93'

Induction of competent cells for Agrobacterium tumefaciens-mediated stable transformation of common bean (Phaseolus vulgaris L.)

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