Agrobacterium tumefaciens-mediated transformation of wheat using a superbinary vector and a polyamine-supplemented regeneration medium

Khanna, Harjeet; Daggard, Grant

doi:10.1007/s00299-002-0529-x

Cited by 86 publications

(59 citation statements)

References 25 publications

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“…In the present study, a 3-day co-cultivation period was found to be optimum for maximum transformation frequency. Similar results were also reported earlier in other species (Hu et al 2002;Khanna et al 2003).…”

Section: Factors Affecting Transformation Frequencysupporting

confidence: 94%

Genetic transformation and regeneration of rubber tree ( Hevea brasiliensis Muell. Arg) transgenic plants with a constitutive version of an anti-oxidative stress superoxide dismutase gene

et al. 2003

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Agrobacterium tumefaciens-mediated genetic transformation and the regeneration of transgenic plants was achieved in Hevea brasiliensis. Immature anther-derived calli were used to develop transgenic plants. These calli were co-cultured with A. tumefaciens harboring a plasmid vector containing the H. brasiliensis superoxide dismutase gene (HbSOD) under the control of the CaMV 35S promoter. The beta-glucuronidase gene (uidA) was used for screening and the neomycin phosphotransferase gene (nptII) was used for selection of the transformed calli. Factors such as co-cultivation time, co-cultivation media and kanamycin concentration were assessed to establish optimal conditions for the selection of transformed callus lines. Transformed calli surviving on medium containing 300 mg l(-1) kanamycin showed a strong GUS-positive reaction. Somatic embryos were then regenerated from these transgenic calli on MS2 medium containing 2.0 mg l(-1) spermine and 0.1 mg l(-1) abscisic acid. Mature embryos were germinated and developed into plantlets on MS4 medium supplemented with 0.2 mg l(-1) gibberellic acid, 0.2 mg l(-1) kinetin (KIN) and 0.1 mg l(-1) indole-3-acetic acid. A transformation frequency of 4% was achieved. The morphology of the transgenic plants was similar to that of untransformed plants. Histochemical GUS assay revealed the expression of the uidA gene in embryos as well as leaves of transgenic plants. The presence of the uidA, nptII and HbSOD genes in the Hevea genome was confirmed by polymerase chain reaction amplification and genomic Southern blot hybridization analyses.

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Section: Factors Affecting Transformation Frequencysupporting

confidence: 94%

Genetic transformation and regeneration of rubber tree ( Hevea brasiliensis Muell. Arg) transgenic plants with a constitutive version of an anti-oxidative stress superoxide dismutase gene

et al. 2003

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“…Biolistics can also be utilised to deliver DNA into the genomes of mitochondria and chloroplasts. Perceived advantages, particularly in terms of lower copy number have driven significant research into using Agrobacterium tumefaciens (synonym Rhizobium radiobacter) for the DNA transfer process, and many laboratories now report successful wheat transformation using Agrobacterium (Binka et al 2012;Campa et al 2005;Cheng et al1997;Cheng et al 2003;Ding et al 2009;Guo et al 1998;Haliloglu and Baenziger 2003;Hamid et al 2012;He et al 2010;Jones et al 2005;Khanna and Daggard 2003;Mahalakshmi and Khurana 1995;Marks et al 1989;McCormac et al 1998;Mitic et al 2004;Mooney et al 1991;Murin et al 2011;Parrott et al 2002;Patnaik et al 2006;Pérez-Piñeiro et al 2012;Przetakiewicz et al 2004;Pukhalskii et al 1996;Rashid et al 2012;Shi et al 2011;Song et al 2012;TamasNyitrai et al 2012;Trifonova et al 2001;Wu et al 2003Wu et al , 2008Xia et al 1999). One particular unpublished method developed by the Japan Tobacco company (PureWheat Technology) has been licensed to specific laboratories who report wheat transformation efficiencies of more than 50 %.…”

Section: Methods For Wheat Transformationmentioning

confidence: 98%

Wheat Biotechnology: Current Status and Future Prospects

Jones

2015

Recent Advancements in Gene Expression and Enabling Technologies in Crop Plants

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In 2010, wheat was grown on nearly 217 million ha worldwide (more than any other food crop) and a total of 651 million t of grain was harvested, making it the third most-cultivated cereal after maize and rice (www.faostat.fao.org). It is one of the main staple food crops providing approximately 20 % of our daily needs of protein and calories. Wheat is traded on global commodity markets which have become accustomed to a good annual harvest and large reserves. However, global demand, estimated at 666 million metric tons (MMT) in 2010, is predicted to increase in line with population growth and reach 880 MMT (a 40 % increase) by 2050 (Weigand 2011). Significant action will be required to maintain this positive situation.Further complicating the supply/demand balance are extreme weather events, unpredictable longer-term climate patterns and changing regional preferences in crop choice/suitability. Recent localised severe weather and poor harvests have resulted in price volatility with notable spikes in 2008 and 2012 (Fig. 8.1). The volatility in the wheat market was exacerbated by the Russian export ban in 2010 resulting from an extreme heatwave in the grain producing regions of southwestern Russia. The highest July temperatures in 130 years brought severe drought, causing the annual harvest of the biggest wheat producing region (Volga) to be reduced by more than 70 % on that of the previous year (Wegren 2011; Welton 2011). The US wheat supplies for 2013/2014 are projected at 2917 million bushels, down 7 % from 2012/2013 (Vocke and Liefert 2014) and there is evidence that growers in some US states are moving away from wheat in favour of biotech soya and corn. Quoting statistics from the US Department of Agriculture, Rookhuyzen (2012) stated that wheat acreage for South Dakota declined by 1.2 million acres, or 28 %, between 1981 and 2011 and the acres planted with spring wheat in 2012 were the lowest in

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“…It has been successfully developed only for the agronomically important wheat (Cheng et al 1997;Hu et al 2003;Khanna and Daggard 2003;Wu et al 2003;Przetakiewicz et al 2004) and more recently for triticale (Nadolska-Orczyk et al 2005). Oat (Avena sativa L.) is another allohexaploid species thus far transformed only via the biolistic method.…”

Section: Introductionmentioning

confidence: 99%

Agrobacterium-mediated transformation of oat (Avena sativa L.) cultivars via immature embryo and leaf explants

et al. 2008

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This paper reports on the successful Agrobacterium-mediated transformation of oat, and on some factors influencing this process. In the first step of the experiments, three cultivars, two types of explant, and three combinations of strain/vectors, which were successfully used for transformation of other cereals were tested. Transgenic plants were obtained from the immature embryos of cvs. Bajka, Slawko and Akt and from leaf base explants of cv. Bajka after transformation with A. thumefaciens strain LBA4404(pTOK233). The highest transformation rate (12.3%) was obtained for immature embryos of cv. Bajka. About 79% of the selected plants proved to be transgenic; however, only 14.3% of the T 0 plants and 27.5% of the T 1 showed GUS expression. Cell competence of both types of explant differed in terms of their transformation ability and transgene expression. The next step of the study was to test the suitability for oat transformation of the pGreen binary vector combined with different selection cassettes: nptII or bar under the nos or 35S promoter. Transgenic plants were selected in combinations transformed with nos::nptII, 35S::nptII and nos::bar. The highest transformation efficiency (5.3%) was obtained for cv. Akt transformed with nos::nptII. A detailed analysis of the T 0 plants selected from a given callus line and their progeny revealed that they were the mixture of transgenic, chimeric-transgenic and non-transgenic individuals. Southern blot analysis of T 0 and T 1 showed simple integration pattern with the low copy number of the introduced transgenes.

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Agrobacterium tumefaciens-mediated transformation of wheat using a superbinary vector and a polyamine-supplemented regeneration medium

Cited by 86 publications

References 25 publications

Genetic transformation and regeneration of rubber tree ( Hevea brasiliensis Muell. Arg) transgenic plants with a constitutive version of an anti-oxidative stress superoxide dismutase gene

Genetic transformation and regeneration of rubber tree ( Hevea brasiliensis Muell. Arg) transgenic plants with a constitutive version of an anti-oxidative stress superoxide dismutase gene

Wheat Biotechnology: Current Status and Future Prospects

Agrobacterium-mediated transformation of oat (Avena sativa L.) cultivars via immature embryo and leaf explants

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