Agrobacterium tumefaciens - mediated transformation of embryogenic avocado cultures and regeneration of somatic embryos

Cruz-Hernández, Andrés; Litz, R. E.; Lim, Mongkol; Witjaksono, Witjaksono

doi:10.1007/s002990050431

Cited by 44 publications

(14 citation statements)

References 20 publications

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“…The transformation efficiency (25.0%) achieved in chestnut using somatic embryos as the target material is similar to that reported for the embryogenic systems of sandalwood (Shiri and Rao 1998), and much higher than those reported for pecan (McGranahan et al 1993) and avocado (Cruz-Hernµndez et al 1998). On the other hand, transformation efficiencies of embryogenic systems as high as 45% in Ginkgo biloba (DuprØ et al 2000) and 40% in Camellia sinensis (Mondal et al 2001) have been reported.…”

Section: Effect Of Antibioticssupporting

confidence: 82%

Agrobacterium-mediated transformation of European chestnut embryogenic cultures

et al. 2004

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An innovative and efficient genetic transformation protocol for European chestnut is described in which embryogenic cultures are used as the target material. When somatic embryos at the globular or early-torpedo stages were cocultured for 4 days with Agrobacterium tumefaciens strain EHA105 harbouring the pUbiGUSINT plasmid containing marker genes, a transformation efficiency of 25% was recorded. Murashige and Skoog culture medium containing 150 mg/l of kanamycin was used as the selection medium. The addition of acetosyringone was detrimental to the transformation efficiency. Transformation was confirmed by a histochemical beta-glucuronidase (GUS ) assay, PCR and Southern blot analyses for the uidA (GUS) and nptII (neomycin phosphotransferase II) genes. At present, 93 GUS-positive chestnut embryogenic lines are being maintained in culture. Low germination rates (6.3%) were recorded for the transformed somatic embryos. The presence of the transferred genes in leaves and shoots derived from the germinated embryos was also verified by the GUS assay and PCR analysis.

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Section: Effect Of Antibioticssupporting

confidence: 82%

Agrobacterium-mediated transformation of European chestnut embryogenic cultures

et al. 2004

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“…Somatic embryogenesis has been used to regenerate genetically transformed trees in a number of species, including Persea americana Mill. (Cruz-Hernández et al 1998), Juglans regia L. (Escobar et al 2000), Pinus radiata Charity et al 2005), Theobroma cacao L. (Maximova et al 2003), Hevea brasiliensis Muell. Arg (Jayashree et al 2003), Pinus pinaster Sol.…”

Section: Introductionmentioning

confidence: 98%

Cyclic secondary somatic embryogenesis and efficient plant regeneration in camphor tree (Cinnamomum camphora L.)

Shi

Dai

et al. 2010

In Vitro Cell.Dev.Biol.-Plant

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An efficient protocol for secondary somatic embryogenesis in camphor tree is reported. Secondary somatic embryos (SSEs), initially obtained from the primary embryos of a nascent embryogenic culture in 2002, were proliferated and maintained for more than 4 yr via cyclic secondary somatic embryogenesis. Throughout this period, the embryo populations retained a high level of competence for plant regeneration. SSEs were produced on the surfaces of the cotyledons and radicular ends of maternal somatic embryos (MSEs). Histological observations of the various stages of secondary embryo development revealed four typical stages, namely, globular, heartshaped, torpedo, and cotyledonary. The process of secondary embryogenesis continued in a cyclic way, with each newly formed embryo producing a subsequent generation of secondary embryos. In order to progress developmentally beyond proliferation cycles, cotyledonary embryos from one of embryogenic lines (L14) were cultured on Murashige and Skoog (MS) medium with 0.1-3.0 mg l −1 abscisic acid (ABA) or 0.05-1.0 mg l −1 thidiazuron (TDZ) in darkness for 2 mo to achieve maturation. Matured embryos were then transferred to MS-based germination medium containing either 0.1 mg l −1 TDZ, 0.2 mg l −1 indole-3-butyric acid (IBA), and 0.5 mg l −1 6-benzylaminopurine (BA) or 0.1 mg l −1 TDZ and 0.2 mg l −1 IBA and were cultured in light for germination. Over 50% of embryos matured in the presence of 0.5 mg l −1 ABA were able to germinate with shoots and poor root system. Frequencies of embryos germinating normal shoots among different genotypes did not change significantly. A total of 93% of the shoots from the germinated embryos converted to plantlets on half strength MS medium with 0.5 mg l −1 IBA by 3 wk. Plantlets acclimatized successfully to ex vitro conditions and developed as field-grown plants with normal appearance.

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“…), a dicotyledonous woody species, is one of the major fruit crops of the tropics and subtropics. Due to its high economic importance, this species is the subject of intensive studies from micropropagation to genetic transformation (PliegoAlfaro 1988;Cruz-Hernandez et al 1998). Various attempts have been made to multiply avocado through tissue culture techniques although this species has certain limitations such as in vitro rooting and ex vitro survival (Pliego-Alfaro 1988; Barceló-Muñoz et al 1999).…”

Section: Introductionmentioning

confidence: 99%

Influences of exogenous sucrose on juvenile avocado during in vitro cultivation and subsequent ex vitro acclimatization

Premkumar

Barceló-Muñóz²,

Pliego‐Alfaro

et al. 2002

Trees

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We investigated changes in physiological characteristics of micropropagated juvenile avocado (Persea americana Mill.) cultured in three different sucrose concentrations (5, 30 and 50 g/l) for 4 weeks and subsequently acclimatized to ex vitro conditions. No significant differences in foliar concentrations of total chlorophylls, N mass , flavonoids and total soluble proteins were observed during in vitro growth or after acclimatization. However, ex vitro transfer exerted multiple effects on foliar ratios of chlorophyll a to b, total chlorophylls to carotenoids and stem N and C/N ratio. Both varying sucrose concentrations during in vitro growth and ex vitro transfer, affected the concentration of rubisco subunits independent of other photosynthetic components, viz. total chlorophylls, foliar N and total soluble proteins. These results suggest that preconditioning juvenile avocado microplants at different concentrations of sucrose modify several physiological parameters during ex vitro acclimatization, like concentrations of carotenoids and rubisco, which can improve vigour of plants in autotrophic conditions.

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Agrobacterium tumefaciens - mediated transformation of embryogenic avocado cultures and regeneration of somatic embryos

Cited by 44 publications

References 20 publications

Agrobacterium-mediated transformation of European chestnut embryogenic cultures

Agrobacterium-mediated transformation of European chestnut embryogenic cultures

Cyclic secondary somatic embryogenesis and efficient plant regeneration in camphor tree (Cinnamomum camphora L.)

Influences of exogenous sucrose on juvenile avocado during in vitro cultivation and subsequent ex vitro acclimatization

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