Agroinoculation of a full-length cDNA clone of cotton leafroll dwarf virus (CLRDV) results in systemic infection in cotton and the model plant Nicotiana benthamiana

Delfosse, Verónica Cecilia; Casse, Maria Florencia; Agrofoglio, Yamila Carla; Kresic, Iván Bonacic; Hopp, H. Esteban; Ziegler-Graff, Véronique; Distéfano, Ana Julia

doi:10.1016/j.virusres.2013.04.007

Cited by 19 publications

(10 citation statements)

References 28 publications

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“…As both CLRDV P0 proteins elicited different pathogenicity responses in a PVX context, it was then assessed whether P0 CL‐at was able to break Cbd resistance when expressed upon CLRDV infection. Gossypium hirsutum ‘NuOpal’ plants, resistant to CLRDV but susceptible to CLRDV‐at, were co‐infiltrated with A. tumefaciens carrying pBin19‐35S/CLRDV (Delfosse et al ., ) and pBin61‐P0 CL , pBin61‐P0 CL‐at or pBin61 empty vector. In parallel, G. hirsutum NC33B plants, susceptible to CLRDV and CLRDV‐at, were co‐infiltrated with pBin19‐35S/CLRDV and pBin61 as infection control (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Conversely to P0 proteins encoded by CABYV, TuYV and CYDV RPV , which were shown to interact with A. thaliana ASK1 and/or ASK2 (Pazhouhandeh et al ., ; Almasi et al ., ), neither P0 CL nor P0 CL‐at bound the A. thaliana factors. This may explain the symptomless infection in agroinoculation experiments of A. thaliana (Delfosse et al ., ). Several studies also reported that polerovirus P0 protein destabilizes AGO1, which results in the reduction of secondary siRNAs accumulation and in the inhibition of the silencing amplification process (Baumberger et al ., ; Bortolamiol et al ., ; Fusaro et al ., ; Almasi et al ., ).…”

Section: Discussionmentioning

confidence: 97%

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P0 protein of cotton leafroll dwarf virus‐atypical isolate is a weak RNA silencing suppressor and the avirulence determinant that breaks the cotton Cbd gene‐based resistance

et al. 2019

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Cotton blue disease (CBD) is the most important disease present in cotton crops in South America and cotton leafroll dwarf virus (CLRDV) is the causal agent. The disease has been controlled by sowing cotton varieties resistant to CLRDV. However, in the 2009/10 growing season, an outbreak due to an atypical CLRDV isolate (CLRDV‐at) occurred in northwest Argentina. Although CLRDV and CLRDV‐at genomes are very closely related, the symptoms they produce in cotton plants are quite different. P0 is the most divergent protein between the isolates and in CLRDV is a silencing suppressor protein. This work characterized the silencing suppressor activity of the P0 protein encoded by CLRDV‐at (P0CL‐at) and evaluated its role in Cbd‐resistance break in cotton plants. It was demonstrated that P0CL‐at, despite having a mutation in the consensus of the F‐box‐like motif, was able to suppress local RNA silencing, but displayed lower activity than P0CL. P0CL and P0CL‐at showed no differences in the interaction with Gossypium hirsutum SKP1 orthologue (GSK1) and Nicotiana benthamiana SKP1 and both P0 proteins triggered destabilization of ARGONAUTE1. However, when the ability to enhance PVX symptoms was evaluated, P0CL‐at was shown to be a weaker pathogenicity factor than P0CL in N. benthamiana. Interestingly, trans‐expressed P0CL‐at enabled CLRDV to systemically infect CBD‐resistant plants, and a chimeric CLRDV‐P0CL‐at infectious clone succeeded in establishing infection in CBD‐resistant cotton varieties with symptoms resembling those produced by CLRDV‐at. These results strongly suggest that P0CL‐at is the avirulence (Avr) determinant involved in breaking cotton Cbd gene‐based resistance.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 97%

P0 protein of cotton leafroll dwarf virus‐atypical isolate is a weak RNA silencing suppressor and the avirulence determinant that breaks the cotton Cbd gene‐based resistance

et al. 2019

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“…It is the most important and principal cash crop that affects India's economy in many ways [9,10]. It is always considered the most economically main crop, but various disease pathogens affect its production [11][12][13]. e largest cotton yielding country is India, as compared to other countries in the entire world, as shown in Figure 1.…”

Section: Background Of Cotton Cropmentioning

confidence: 99%

A Comparative Analysis of Machine Learning Algorithms for Detection of Organic and Nonorganic Cotton Diseases

Kumar¹,

Jain

Shukla

et al. 2021

Mathematical Problems in Engineering

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Cotton is the natural fiber produced, and the commercial crop grown in monoculture on 2.5% of total agricultural land. Cotton is a drought-resistant crop that provides a reliable income to the farmers that grow under the area with a threat from climatic change. These cotton crops are being affected by bacterial, fungal, viral, and other parasitic diseases that may vary due to the climatic conditions resulting in the crop’s low productivity. The most prone to diseases is the leaf that results in the damage of the plant and sometimes the whole crop. Most of the diseases occur only on leaf parts of the cotton plant. The primary purpose of disease detection has always been to identify the diseases affecting the plant in the early stages using traditional techniques for better production. To detect these cotton leaf diseases appropriately, the prior knowledge and utilization of several image processing methods and machine learning techniques are helpful.

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“…DNA‐layered RNA replicons are frequently used in laboratories to initiate infection in host plants if viruses cannot be transmitted via mechanical inoculation. For example, cotton leaf roll dwarf virus cDNA, controlled by a CaMV35S promoter, was agroinfiltrated into cotton or N. benthamiana leaves, and it initiated systemic infection that was successfully transmitted to non‐infected plants via its specific vector Aphis gossypii . Similarly, agroinfiltrated plants with a genetic construct that included beet necrotic virus yellow vein virus cDNA were systemically infected, and the virus was transmitted to healthy plants via Polymyxa betae .…”

Section: Plants May Produce Replicons That Are Infectious To Other Plmentioning

confidence: 99%

The alien replicon: Artificial genetic constructs to direct the synthesis of transmissible self‐replicating RNAs

Кочетов

2014

BioEssays

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Artificial genetic constructs that direct the synthesis of self-replicating RNA molecules are used widely to induce gene silencing, for bioproduction, and for vaccination. Interestingly, one variant of the self-replicon has not been discussed in the literature: namely, transgenic organisms that synthesise alien replicons. For example, plant cells may be easily genetically modified to produce bacteriophages or insect viruses. Alien replicon-producing organisms (ARPOs) may serve as a unique tool for biocontrol or to selectively influence the characteristics of a target organism. The ARPO approach would have to meet strict biosafety criteria, and its practical applications are problematic. However, a discussion on ARPO applicability would be valuable to outline the full set of options available in the bioengineering toolbox. In this paper, RNA replicons for bioengineering are reviewed briefly, and the ARPO approach is discussed.

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Agroinoculation of a full-length cDNA clone of cotton leafroll dwarf virus (CLRDV) results in systemic infection in cotton and the model plant Nicotiana benthamiana

Cited by 19 publications

References 28 publications

P0 protein of cotton leafroll dwarf virus‐atypical isolate is a weak RNA silencing suppressor and the avirulence determinant that breaks the cotton Cbd gene‐based resistance

P0 protein of cotton leafroll dwarf virus‐atypical isolate is a weak RNA silencing suppressor and the avirulence determinant that breaks the cotton Cbd gene‐based resistance

A Comparative Analysis of Machine Learning Algorithms for Detection of Organic and Nonorganic Cotton Diseases

The alien replicon: Artificial genetic constructs to direct the synthesis of transmissible self‐replicating RNAs

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