Agromyces laixinhei sp. nov. isolated from bat feces in China

Abstract

Three rod-shaped, Gram-stain-positive, and catalase-positive, phenotypically closely related isolates (HY052 T , HY050, and HY045) were obtained from fecal samples collected from bats in Guangxi province and Chongqing city of China. Circular, smooth, light-yellow colonies appeared on brain heart infusion plate after 24–48 h incubation at 28°C. The optimal pH for growth was between 6.0 and 7.5. Based on 16S rRNA, the three isolates were phylogenetically related to Agromyces terreus DS-10 T , Agromyces aureus AR33 T , Agromyces salentinus 20-5 T , Agromyces allii UMS-62 T , Agromyces lapidis CD55 T , and Agromyces italicus CD1 T . Moreover, based on 296 core genes, the phylogenomic tree indicated that the three isolates clustered together, closest to Agromyces cerinus VKM Ac-1340 T and Agromyces fucosus VKM Ac-1345 T but separated distantly from other Agromyces species. The average nucleotide identity values between strain HY052 T and other Agromyces species ranged from 79.3% to 87.9%, lower than the 95–96% threshold. Furthermore, the genome of strain HY052 T contains a circular chromosome of 3,437,203 bp with G + C content of 69.0 mol%. Main fatty acids were anteiso -C 15:0 and anteiso -C 17:0 . The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, and unidentified glycolipids. Rhamnose, ribose, and glucose were the primary cell wall sugars. The major peptidoglycan amino acids included alanine, glutamic acid, glycine, and 2,4-diaminobutyric acid. An additional remarkable difference from other Agromyces species is that MK-12 was the sole menaquinone in strain HY052 T . Based on results from the polyphasic characterizations performed in this study, our isolates are proposed to be members of a novel species in genus Agromyces , named Agromyces laixinhei. The type strain is HY052 T (= CGMCC 1.17175 T = JCM 33695 T ). Electronic Supplementary Material Supplementary material is available in the online version of this article at 10.1007/s12275-021-0546-5.

Phenotypic and genomic characteristics of Brevibacterium zhoupengii sp. nov., a novel halotolerant actinomycete isolated from bat feces

“…However, bat-associated bacteria were relatively neglected (Muhldorfer, 2013;Afonso and Goydadin, 2018), which might play an important role as components of the microbiota. Some novel bacterial species previously isolated from bat feces in our laboratory belong to the phylum Actinobacteria, such as Agromyces laixinhei (Cheng et al, 2021), Haloactinobacterium kanbiaonis (Xu et al, 2021), Microbacterium chengjingii, M. fandaimingii (Zhou et al, 2021), and Ruania zhangjianzhongii (Xu et al, 2021). In the present study, we continue to explore bat fecal microbiota, and describe the phenotypic and genomic characterization of a novel species in the genus Brevibacterium belonging to phylum Actinobacteria.…”

“…), which were collected from the Chongzuo city, Guangxi province (22°20′54″N, 106°49′20″E, July 2011) and Chuxiong Yi Autonomous Prefecture, Yunnan province (25°09′10″N, 102°04′39″E, October 2013), respectively. Sample collection, storage, transportation and strain isolation were similar to what was pre-viously described (Cheng et al, 2021;Huang et al, 2022). In brief, bat feces from the same location were pooled together, fully ground, well mixed on a 1.5 ml tube rotator, and 150 μl of each mixed sample (1.0 g diluted with 1.0 ml 0.85% [w/v] NaCl solution) was immediately plated onto the brainheart infusion (BHI) plates supplemented with 5% sheep blood (BHIB).…”

Agromyces archimandritae sp. nov., isolated from the cockroach Archimandrita tessellata

“…2) showed G127AT T to be part of the ancestral lieage of the genus Agromyces, and only Agromyces atrinae P27 T was found to diverge earlier than G127AT T . The early divergence of A. atrinae P27 T has also been found in the phylogenomic tree based on 296 core genes reported for 'Agromyces laixinhei' HY052 [32]. The pairwise genomic distance from G127AT T to the type strains of species of the genus Agromyces was calculated using both DNA and protein-based genome relatedness indices.…”

“…The early divergence of A. atrinae P27 T has also been found in the phylogenomic tree based on 296 core genes reported for ‘ Agromyces laixinhei’ HY052 [32]. The pairwise genomic distance from G127AT T to the type strains of species of the genus Agromyces was calculated using both DNA and protein-based genome relatedness indices.…”

The Threat of Potentially Pathogenic Bacteria in the Feces of Bats

“…Bacteria were isolated as described previously ( 67 , 79 ). In brief, bat fecal samples collected from the same place were mixed and ground.…”