Agronomic and seed traits of high‐oleic soybean lines containing the DP‐305423‐1 transgene in four backcross populations

Abstract

The DP‐305423‐1 transgene elevates oleic acid concentration and reduces polyunsaturated fatty acids in soybean [Glycine max (L.) Merr.] oil, resulting in increased oxidative stability. The objective of this research was to investigate the effect of the DP‐305423‐1 transgene on agronomic and seed traits using four backcross derived soybean populations to provide further understanding of the transgene's influence across varying genetic backgrounds and environments. An equal number of high‐oleic, low‐linolenic (HOLL) and normal‐oleic, low‐linolenic (NOLL) progeny from four BC1F4:F6 populations—B (n = 30), C (n = 22), D (n = 56), and E (n = 34)—were grown across six locations in 2015. Across all locations, the high‐oleic (HO) progeny had a mean yield 77 kg ha−1 less in Population B, and 382 kg ha−1 greater in Population C compared with the normal‐oleic (NO) class. There was no significant difference in yield between HO and NO in Populations D and E. The HO progeny had a significantly lower mean oil concentration and greater protein concentration than the NO class in all populations, whereas 100‐seed weight of the HO class was, on average, greater than in the NO class. The results provide evidence that the reduction in oil concentration is associated with the HO phenotype in the progeny carrying the DP‐3054231‐1 transgene and demonstrate no consistent effect of the transgene on seed yield, suggesting that differences may be due to the genetic background of the population studied.

Accuracy of genomic prediction for seed oil concentration in high‐oleic soybean populations using a low‐density marker panel

“…Breeding for elevated oil concentration in high-oleic soybean germplasm with the DP-305423-1 transgene is an important target. It has been demonstrated that high-oleic progeny have lower oil concentration than full-sib normal oleic progeny (Brace et al, 2011;Hemingway et al, 2020;Spear et al, 2012), and it is conceivable that increasing oil concentration may have a positive impact on the commercial production of high-oleic cultivars. With more oil extracted from a unit of grain, oil processors will have lower costs per unit of oil and higher financial returns resulting in greater financial incentives for soybean producers to grow a higholeic cultivar.…”