Agronomic Applications of
            <i>Azospirillum</i>
            and Other PGPR

Okon, Yaacov; Labandera‐Gonzales, Carlos; Lage, Martin; Lage, Pedro

doi:10.1002/9781119053095.ch90

Cited by 24 publications

(24 citation statements)

References 17 publications

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“…Phosphate determination was made by the vanadomolybdate colorimetric method according to Pearson (1976). The strain Pseudomonas fluorescens BNM 233 currently used as a biofertilizer (Okon et al, 2015) was used as a positive control for phosphate solubilization.…”

Section: Assays For Detection Of Pgp Abilitiesmentioning

confidence: 99%

Methylobacterium sp. 2A Is a Plant Growth-Promoting Rhizobacteria That Has the Potential to Improve Potato Crop Yield Under Adverse Conditions

et al. 2020

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A Gram-negative pink-pigmented bacillus (named 2A) was isolated from Solanum tuberosum L. cv. Desirée plants that were strikingly more developed, presented increased root hair density, and higher biomass than other potato lines of the same age. The 16S ribosomal DNA sequence, used for comparative gene sequence analysis, indicated that strain 2A belongs to the genus Methylobacterium. Nucleotide identity between Methylobacterium sp. 2A sequenced genome and the rest of the species that belong to the genus suggested that this species has not been described so far. In vitro, potato plants inoculated with Methylobacterium sp. 2A had a better performance when grown under 50 mM NaCl or when infected with Phytophthora infestans. We inoculated Methylobacterium sp. 2A in Arabidopsis thaliana roots and exposed these plants to salt stress (75 mM NaCl). Methylobacterium sp. 2A-inoculated plants, grown in control or salt stress conditions, displayed a higher density of lateral roots (p < 0.05) compared to noninoculated plants. Moreover, under salt stress, they presented a higher number of leaves and larger rosette diameter. In dual confrontation assays, Methylobacterium sp. 2A displayed biocontrol activity against P. infestans, Botrytis cinerea, and Fusarium graminearum, but not against Rhizoctonia solani, and Pythium dissotocum. In addition, we observed that Methylobacterium sp. 2A diminished the size of necrotic lesions and reduced chlorosis when greenhouse potato plants were infected with P. infestans. Methylobacterium sp. 2A produces indole acetic acid, solubilizes mineral phosphate and is able to grow in a N 2 free medium. Whole-genome sequencing revealed metabolic pathways associated with its plant growth promoter capacity. Our results suggest that Methylobacterium sp. 2A is a plant growth-promoting rhizobacteria (PGPR) that can

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Section: Assays For Detection Of Pgp Abilitiesmentioning

confidence: 99%

Methylobacterium sp. 2A Is a Plant Growth-Promoting Rhizobacteria That Has the Potential to Improve Potato Crop Yield Under Adverse Conditions

et al. 2020

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“…Not only were the isolated endophytic bacteria related to hydrocarbon degradation but additional plant growth promoting abilities are associated with other orders such as Actinomycetales, Rhizobiales, Bacilliales, Burkholderiales, and Enterobacteriales. All of these taxa are associated with either the production of 1-aminocyclopropane-1-carboxylate deaminase and indole acetic acid (Saif et al 2014;Yaish et al 2015), siderophore production (Jetiyanon 2015;Prasad et al 2015), phosphate solubilisation (Saif et al 2014;Jetiyanon 2015), or nitrogen fixation (Okon et al 2015).…”

Section: Identification Of Culturable Bacteriamentioning

confidence: 99%

Endophytic root bacteria associated with the natural vegetation growing at the hydrocarbon-contaminated Bitumount Provincial Historic site

2017

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Abstract:The Bitumount Provincial Historic site is the location of 2 of the world's first oil-extracting and -refining operations. Despite hydrocarbon levels ranging from 330 to 24 700 mg·(kg soil) −1 , plants have been able to recolonize the site through means of natural revegetation. This study was designed to achieve a better understanding of the plant-root-associated bacterial partnerships occurring within naturally revegetated hydrocarboncontaminated soils. Root endophytic bacterial communities were characterized from representative plant species throughout the site by both high-throughput sequencing and culturing techniques. Population abundance of rhizosphere and root endosphere bacteria was significantly influenced (p < 0.05) by plant species and sampling location. In general, members of the Actinomycetales, Rhizobiales, Pseudomonadales, Burkholderiales, and Sphingomonadales orders were the most commonly identified orders. Community structure of root-associated bacteria was influenced by both plant species and sampling location. Quantitative real-time polymerase chain reaction was used to determine the potential functional diversity of the root endophytic bacteria. The gene copy numbers of 16S rRNA and 2 hydrocarbon-degrading genes (CYP153 and alkB) were significantly affected (p < 0.05) by the interaction of plant species and sampling location. Our findings suggest that some of the bacterial communities detected are known to exhibit plant growth promotion characteristics.Key words: reclamation-remediation, endophytic root bacteria, petroleum hydrocarbons, microbial community.

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“…), however few have been explored for the production of commercial inoculants, with emphasis on some species of Azospirillum that are mainly, but not exclusively, used in cultures for cereals such as wheat, rice and maize (Okon et al . ; Cassán & Diaz‐Zorita ). Due to its many growth‐promoting abilities, an additive hypothesis was formulated to define the mode of action of Azospirillum in plant development (Bashan & de‐Bashan ).…”

Section: Introductionmentioning

confidence: 99%

“…These mechanisms include direct effects on plant metabolism, by providing nutrients (iron, phosphorus and N, for example) and/or synthesising molecules with phytohormone activity (auxins, cytokinins, nitric oxide), or indirect effects such as antagonism against phytopathogens and increased resistance to environmental stresses (Glick 2012). A large diversity of associative bacterial species has been described as potential PGPB (Vejan et al 2016), however few have been explored for the production of commercial inoculants, with emphasis on some species of Azospirillum that are mainly, but not exclusively, used in cultures for cereals such as wheat, rice and maize (Okon et al 2015;Cass an & Diaz-Zorita 2016). Due to its many growth-promoting abilities, an additive hypothesis was formulated to define the mode of action of Azospirillum in plant development (Bashan & de-Bashan 2010).…”

Section: Introductionmentioning

confidence: 99%

Associative bacteria influence maize (Zea mays L.) growth, physiology and root anatomy under different nitrogen levels

et al. 2018

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Despite the great diversity of plant growth-promoting bacteria (PGPB) with potential to partially replace the use of N fertilisers in agriculture, few PGPB have been explored for the production of commercial inoculants, reinforcing the importance of identifying positive plant-bacteria interactions. Aiming to better understand the influence of PGPB inoculation in plant development, two PGPB species with distant phylogenetic relationship were inoculated in maize. Maize seeds were inoculated with Bacillus sp. or Azospirillum brasilense. After germination, the plants were subjected to two N treatments: full (N+) and limiting (N-) N supply. Then, anatomical, biometric and physiological analyses were performed. Both PGPB species modified the anatomical pattern of roots, as verified by the higher metaxylem vessel element (MVE) number. Bacillus sp. also increased the MVE area in maize roots. Under N+ conditions, both PGPB decreased leaf protein content and led to development of shorter roots; however, Bacillus sp. increased root and shoot dry weight, whereas A. brasilense increased photosynthesis rate and leaf nitrate content. In plants subjected to N limitation (N-), photosynthesis rate and photosystem II efficiency increased in maize inoculated with Bacillus sp., whilst A. brasilense contained higher ammonium, amino acids and total soluble sugars in leaves, compared to the control. Plant developmental and metabolical patterns were switched by the inoculation, regardless of the inoculant bacterium used, producing similar as well as distinct modifications to the parameters studied. These results indicate that even non-diazotrophic inoculant strains can improve the plant N status as result of the morpho-anatomical and physiological modifications produced by the PGPB.

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Agronomic Applications of Azospirillum and Other PGPR

Cited by 24 publications

References 17 publications

Methylobacterium sp. 2A Is a Plant Growth-Promoting Rhizobacteria That Has the Potential to Improve Potato Crop Yield Under Adverse Conditions

Methylobacterium sp. 2A Is a Plant Growth-Promoting Rhizobacteria That Has the Potential to Improve Potato Crop Yield Under Adverse Conditions

Endophytic root bacteria associated with the natural vegetation growing at the hydrocarbon-contaminated Bitumount Provincial Historic site

Associative bacteria influence maize (Zea mays L.) growth, physiology and root anatomy under different nitrogen levels

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