“…The lower spike density of this study may have limited competition between spikes and maintained relatively higher spike fertility. All in all, this sustains the hypothesis that yield and harvest index decline with stand age such as observed in other studies (Jungers et al, 2017;Pugliese, 2017;Frahm et al, 2018;Tautges et al, 2018;Bajgain et al, 2020;Locatelli et al, 2023) might partly be caused by the increasing competition in stands with excessive tiller density (Law et al, 2020;Cassman & Connor, 2022). While tillering is necessary for aboveground biomass production, selection for grain yield improvement should focus on the reduction of excessive tillering (incl.…”