Agronomic biofortification increases concentrations of zinc and storage proteins in common bean (Phaseolus vulgaris L.) grains

Kachinski, Wagner Deckij; Ávila, Fabrício William; Reis, André Rodrigues dos; Müller, Marcelo Marques Lopes; Mendes, Marcelo Cruz; Petranski, Poliana Horst

doi:10.1016/j.foodres.2022.111105

Cited by 14 publications

(11 citation statements)

References 35 publications

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“…In contrast, previous studies reported a beneficial influence of foliar-applied zinc sulfate on the concentration of total sugars in seeds of pea [ 43 ] and black gram [ 44 ]. Another study found both non-significant and beneficial effects of foliar-applied zinc sulfate on the concentration of total sugars in seeds of common bean [ 45 ]. In the present study, non-beneficial effects of Se treatments on soluble solids concentration in pea seed ( Figure 1 A) may be associated with reduced seed chlorophyll concentration ( Table 3 ).…”

Section: Discussionmentioning

confidence: 99%

Separate Effects of Foliar Applied Selenate and Zinc Oxide on the Accumulation of Macrominerals, Macronutrients and Bioactive Compounds in Two Pea (Pisum sativum L.) Seed Varieties

Malka

Laing

Bohn

2022

Plants

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Selenium (Se) and zinc (Zn) are important cofactors for antioxidant enzymes. Foliar Se/Zn application is a highly efficient strategy of plant biofortification. However, its effect on the accumulation of macrominerals, macronutrients and bioactive compounds in the pea plant (Pisum sativum L.) have been poorly investigated. A two-year pot experiment was performed to study responses of two pea varieties (Ambassador, Premium) to foliar-applied sodium selenate (0/50/100 g Se/ha) and zinc oxide (0/375/750 g Zn/ha) at the flowering stage. Concentrations of Ca, Mg, K, Na, soluble solids (SSC), protein, chlorophyll a and b, total chlorophyll, total carotenoids and total condensed tannins (TCT) were determined in seeds. Mg concentration in Ambassador and chlorophyll a concentration in Premium were positively affected, in part, by selenate and zinc oxide, respectively. Selenate and zinc oxide increased, in part, protein concentration in Premium. Highest protein concentration was found in Premium treated with 375 g Zn/ha (27.6% DW) vs. the control (26.6% DW). Significant (all p < 0.001) positive correlations were found, among others, between concentrations of Zn and Mg (r2 = 0.735) and between Zn and protein (r2 = 0.437) for Ambassador, and between Mg and protein (r2 = 0.682), between Zn and Mg (r2 = 0.807), as well as between Zn and protein (r2 = 0.884) for Premium. TCT significantly (all < 0.05) and positively correlated with SSC (r2 = 0.131), chlorophyll b (r2 = 0.128) and total chlorophyll (r2 = 0.109) for Ambassador. This study provides new nutritional data on Se/Zn biofortified peas, important for improving agronomic biofortification of pea plants.

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Section: Discussionmentioning

confidence: 99%

Separate Effects of Foliar Applied Selenate and Zinc Oxide on the Accumulation of Macrominerals, Macronutrients and Bioactive Compounds in Two Pea (Pisum sativum L.) Seed Varieties

Malka

Laing

Bohn

2022

Plants

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“…Selenate and zinc oxide did not produce beneficial changes on the levels of soluble solids in pea seeds ( Table 2 , Figure 1 F), which is consistent with our pot study [ 34 ]. However, previous studies did report increased concentrations of total sugars in seeds of pea [ 49 ], black gram [ 50 ], and common bean [ 51 ] following zinc sulphate application. Soluble solids are mostly simple carbohydrates that are needed for seed development [ 49 ].…”

Section: Discussionmentioning

confidence: 99%

Foliar Selenate and Zinc Oxide Separately Applied to Two Pea Varieties: Effects on Growth Parameters and Accumulation of Minerals and Macronutrients in Seeds under Field Conditions

Malka

Laing

Hegedüsová

et al. 2023

Foods

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Though selenium (Se) and zinc (Zn) constitute essential nutrients for human health, their deficiencies affect up to 15% and 17% of the global population, respectively. Agronomic biofortification of staple crops with Se/Zn may alleviate these challenges. Pea (Pisum sativum L.) is a nutritious legume crop that has great potential for Se/Zn biofortification. Herein, two varieties of pea (Ambassador, Premium) were biofortified via foliar application of sodium selenate (0/50/100 g of Se/ha) or zinc oxide (0/375/750 g of Zn/ha) during the flowering stage under field conditions. While no significant differences were found in Se accumulation between seed varieties upon Se treatments, selenate enhanced the accumulation of Se in the two seed varieties in a dose dependent manner. Selenium concentration was most elevated in seeds of Ambassador exposed to 100 g of Se/ha (3.93 mg/kg DW compared to the control (0.08 mg/kg DW), p < 0.001). 375 g of Zn/ha (35.7 mg/kg DW) and 750 g of Zn/ha (35.5 mg/kg DW) significantly and similarly enhanced Zn concentrations compared to the control (31.3 mg/kg DW) in Premium seeds, p < 0.001. Zinc oxide also improved accumulations of Fe, Cu, Mn, and Mg in Premium seeds. Se/Zn treatments did not significantly affect growth parameters and accumulations of soluble solids and protein in seeds. Positive and significant (p < 0.01) correlations were observed between Zn and Fe, Cu, Mn and Mg levels in Premium seeds, among others. Consuming 33 g/day of pea biofortified with Se at 50 g/ha and 266 g/day of pea biofortified with 375 g of Zn/ha could provide 100% of the RDA (55 μg) for Se and RDA (9.5 mg) for Zn in adults, respectively. These results are relevant for enhancing Se/Zn status in peas by foliar biofortification.

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“…Due to the different specific surface areas, surface electrical properties, and hydrophobic interaction with adsorbed substances, different resins have a significant impact on the adsorption capacity of antioxidant peptides in PSKB (21,22). The structural parameters of the three macroporous adsorption resins selected in the test are shown in Table 1.…”

Section: Screening Of Macroporous Adsorption Resinmentioning

confidence: 99%

Separation and purification of antioxidant peptides from purple speckled kidney bean by macroporous adsorption resin and analysis of amino acid composition

Yang

et al. 2022

Front. Nutr.

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The protein hydrolysate of purple speckled kidney bean (PSKB) was used as the raw material in this study, and the antioxidant peptide of the PSKB protein hydrolysate was purified using macroporous resin. The XAD-7HP macroporous resin was selected as the best purification material, and the static adsorption-desorption of the purified PSKB antioxidant peptide was optimized. The optimum static adsorption and desorption conditions were as follows: the adsorption capacity reached 11.93 ± 0.11 mg/ml at pH 7 for 24 h, and the desorption capacity was 5.24 ± 0.04 mg/ml with 60% ethanol for 30 min. Under this condition, the amount of antioxidant peptide obtained by adsorption-desorption was the highest. The optimum process conditions were as follows: the appropriate flow rate was 1 ml/min, and the optimal injection volume was 40 ml. The adsorption amount at this time can reach 12.19 ± 0.15 mg/ml. The components with an elution time of 10–30 min were separated using the reversed-phase high-performance liquid chromatography (RP-HPLC) technique to obtain three main components, namely, RP1, RP2, and RP3. The DPPH free radical scavenging ability reached 56.26 ± 0.56, 66.42 ± 0.56, and 78.57 ± 0.56%, respectively, which were 36.65, 46.34 ± 0.56, and 54.39 ± 0.56% higher than those before purification. The amino acid sequences of the three components were identified as Phe-Leu-Val-Asp-Arg-Ile, Phe-Leu-Val-Ala-Pro-Asp-Asp, and Lys-Asp-Arg-Val-Ile-Ser-Glu-Leu.

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Agronomic biofortification increases concentrations of zinc and storage proteins in common bean (Phaseolus vulgaris L.) grains

Cited by 14 publications

References 35 publications

Separate Effects of Foliar Applied Selenate and Zinc Oxide on the Accumulation of Macrominerals, Macronutrients and Bioactive Compounds in Two Pea (Pisum sativum L.) Seed Varieties

Separate Effects of Foliar Applied Selenate and Zinc Oxide on the Accumulation of Macrominerals, Macronutrients and Bioactive Compounds in Two Pea (Pisum sativum L.) Seed Varieties

Foliar Selenate and Zinc Oxide Separately Applied to Two Pea Varieties: Effects on Growth Parameters and Accumulation of Minerals and Macronutrients in Seeds under Field Conditions

Separation and purification of antioxidant peptides from purple speckled kidney bean by macroporous adsorption resin and analysis of amino acid composition

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