“…Reduction in the need for N fertilizer inputs with application of animal manures has been oft observed due to significant N mineralization (Eghball, 2000; Jokela, 1992; Mitchell & Tu, 2005; Tewolde, Sistani, Feng, & Menkir, 2019). Determining the appropriate level of N fertilizer reduction with animal manure application is complicated by source of manure, variation in nutrient concentration of manures from the same source, handling of manure prior to application, and weather conditions following application that affect decomposition and release of nutrients (Eghball, Wienhold, Gilley, & Eigenberg, 2002; Larney & Hao, 2007; Zavattaro et al., 2017). Summarizing this comparison, application of organic amendment was manifested in greater soil organic C and N fractions (particularly net N mineralization and soil‐test biological activity) that contributed to effective nutrient cycling and reduced the need for synthetic N inputs.…”